Artículo

Castilla, V.; Mersich, S.E.; Candurra, N.A.; Damonte, E.B. "The entry of Junin virus into Vero cells" (1994) Archives of Virology. 136(3-4):363-374
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Abstract:

The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion. © 1994 Springer-Verlag.

Registro:

Documento: Artículo
Título:The entry of Junin virus into Vero cells
Autor:Castilla, V.; Mersich, S.E.; Candurra, N.A.; Damonte, E.B.
Filiación:Laboratorio de Virología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:amantadine; ammonium chloride; chlorpheniramine; procaine; virus protein; animal; article; biosynthesis; drug effect; fluorescent antibody technique; Junin virus; membrane fusion; pH; physiology; Vero cell; virus replication; Amantadine; Ammonium Chloride; Animal; Chlorpheniramine; Fluorescent Antibody Technique; Hydrogen-Ion Concentration; Junin virus; Membrane Fusion; Procaine; Support, Non-U.S. Gov't; Vero Cells; Viral Proteins; Virus Replication
Año:1994
Volumen:136
Número:3-4
Página de inicio:363
Página de fin:374
DOI: http://dx.doi.org/10.1007/BF01321064
Título revista:Archives of Virology
Título revista abreviado:Arch. Virol.
ISSN:03048608
CODEN:ARVID
CAS:amantadine, 665-66-7, 768-94-5; ammonium chloride, 12125-02-9; chlorpheniramine, 132-22-9; procaine, 51-05-8, 59-46-1; Amantadine, 768-94-5; Ammonium Chloride, 12125-02-9; Chlorpheniramine, 132-22-9; Procaine, 59-46-1; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v136_n3-4_p363_Castilla

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Citas:

---------- APA ----------
Castilla, V., Mersich, S.E., Candurra, N.A. & Damonte, E.B. (1994) . The entry of Junin virus into Vero cells. Archives of Virology, 136(3-4), 363-374.
http://dx.doi.org/10.1007/BF01321064
---------- CHICAGO ----------
Castilla, V., Mersich, S.E., Candurra, N.A., Damonte, E.B. "The entry of Junin virus into Vero cells" . Archives of Virology 136, no. 3-4 (1994) : 363-374.
http://dx.doi.org/10.1007/BF01321064
---------- MLA ----------
Castilla, V., Mersich, S.E., Candurra, N.A., Damonte, E.B. "The entry of Junin virus into Vero cells" . Archives of Virology, vol. 136, no. 3-4, 1994, pp. 363-374.
http://dx.doi.org/10.1007/BF01321064
---------- VANCOUVER ----------
Castilla, V., Mersich, S.E., Candurra, N.A., Damonte, E.B. The entry of Junin virus into Vero cells. Arch. Virol. 1994;136(3-4):363-374.
http://dx.doi.org/10.1007/BF01321064