Artículo
Stella, A.M.; del C. Batlle, A.M. "Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis" (1978) Plant Science Letters. 11(2):87-92
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Abstract:
Highly stable selective adsorbents for δ-aminolaevulinate dehydratase (ALA-D) were prepared by attaching the substrate to agarose beads, either directly or through an extension arm. Columns containing these biospecific adsorbents can completely bind the enzyme present in extracts of Euglena gracilis Z strain. Elution is obtained by changing the ionic strength of the buffer. Due to their ease of preparation and high stability these adsorbents may be of general value for purification of ALA-D from different sources. © 1978.
Registro:
| Documento: | Artículo |
| Título: | Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis |
| Autor: | Stella, A.M.; del C. Batlle, A.M. |
| Filiación: | Laboratorio de Porfirinas, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, 1428 Nuńez Buenos Aires, Argentina |
| Año: | 1978 |
| Volumen: | 11 |
| Número: | 2 |
| Página de inicio: | 87 |
| Página de fin: | 92 |
| DOI: | http://dx.doi.org/10.1016/0304-4211(78)90110-4 |
| Título revista: | Plant Science Letters |
| ISSN: | 03044211 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p87_Stella |
Referencias:
- Müller, Lissner, Ippen, (1974) Pyrrole Information, 2, p. 17
- Stella, del C. Batlle, (1977) Int. J. Biochem., 8, p. 353
- Mutthukrishnan, Padmanaban, Sarma, (1969) J. Biol. Chem., 244, p. 4241
- Ebbon, Tait, (1969) Biochem. J., 111, p. 573
- Shemin, 5-Aminolaevulinic Acid Dehydratase: Structure, Function, and Mechanism [and Discussion] (1976) Philosophical Transactions of the Royal Society B: Biological Sciences, 273, p. 75
- Cuatrecasas, (1971) Biochemical Aspects of Reactions on Solid Supports, p. 79. , G.R. Stark, Academic Press, New York
- Rossetti, del C. Batlle, (1977) Int. J. Biochem., 8, p. 268
- Tigier, del C. Batlle, Locascio, (1970) Enzymology, 38, p. 43
- Nandi, Shemin, (1968) J. Biol. Chem., 243, p. 1236
Citas:
---------- APA ----------
Stella, A.M. & del C. Batlle, A.M. (1978) . Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis. Plant Science Letters, 11(2), 87-92.http://dx.doi.org/10.1016/0304-4211(78)90110-4
---------- CHICAGO ----------
Stella, A.M., del C. Batlle, A.M. "Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis" . Plant Science Letters 11, no. 2 (1978) : 87-92.http://dx.doi.org/10.1016/0304-4211(78)90110-4
---------- MLA ----------
Stella, A.M., del C. Batlle, A.M. "Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis" . Plant Science Letters, vol. 11, no. 2, 1978, pp. 87-92.http://dx.doi.org/10.1016/0304-4211(78)90110-4
---------- VANCOUVER ----------
Stella, A.M., del C. Batlle, A.M. Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis. 1978;11(2):87-92.http://dx.doi.org/10.1016/0304-4211(78)90110-4
