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Abstract:

An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose: glycogen glucosyltransferase as compared with those of the already known UDP glucose: glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-[14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after α or β amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent on the presence of a 'primer' of the α 1 → 4 glucan type. © 1978.

Registro:

Documento: Artículo
Título:Glycogen synthesis in the fungus Neurospora crassa
Autor:Fontana, J.D.; Krisman, C.R.
Filiación:Instituto de Investigaciones Bioquimicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:glycogen; radioisotope; adenosine diphosphate glucose c 14; animal experiment; fungus; glucose c 14; in vitro study; neurospora crassa; Adenosine Diphosphate Glucose; Comparative Study; Enzyme Activation; Glycogen Synthase; Neurospora; Neurospora crassa; Uridine Diphosphate Glucose
Año:1978
Volumen:540
Número:2
Página de inicio:183
Página de fin:189
DOI: http://dx.doi.org/10.1016/0304-4165(78)90130-7
Título revista:BBA - General Subjects
Título revista abreviado:Biochim. Biophys. Acta Gen. Subj.
ISSN:03044165
CODEN:BBGSB
CAS:glycogen, 9005-79-2; Adenosine Diphosphate Glucose, 2140-58-1; Glycogen Synthase, EC 2.4.1.11; Uridine Diphosphate Glucose, 133-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v540_n2_p183_Fontana

Referencias:

  • Téllez-Inón, Terenzi, Torres, Interconvertible forms of glycogen synthetase in Neurospora crassa (1969) Biochimica et Biophysica Acta (BBA) - Enzymology, 191, pp. 765-768
  • Mishra, Tatum, (1972) Proc. Natl. Acad. Sci. U.S., 69, pp. 313-317. , 5th edn
  • Stalmans, Hers, (1973) The Enzymes, 9, pp. 309-361. , 5th edn., P.D. Boyer, Academic Press, New York
  • Wright, Robbins, (1965) Biochim. Biophys. Acta, 104, pp. 594-596
  • Recondo, Dankert, Leloir, (1963) Biochem. Biophys. Res. Commun., 12, pp. 204-207
  • Bourne, Donnison, Haworth, Peat, 345. Thymol and cyclohexanol as fractionating agents for starch (1948) Journal of the Chemical Society (Resumed), pp. 1687-1693
  • Lowry, Rosebrough, Farr, Randall, (1961) J. Biol. Chem., 193, pp. 265-275
  • Krisman, (1962) Anal. Biochem., 4, pp. 17-23
  • Barrat, Ogata, (1972) Neurospora Newsl., 19, pp. 34-105
  • Flawiá, Terenzi, Torres, (1977) Arch. Biochem. Biophys., 180, pp. 334-342
  • Barrat, (1966) Neurospora Newsl., 10, pp. 33-35
  • Barengo, Flawiá, Krisman, (1975) FEBS Lett., 53, pp. 274-278
  • Piras, Rothman, Cabib, (1968) Biochemistry, 7, pp. 55-66
  • Bray, (1960) Anal. Biochem., 1, pp. 279-285
  • Krebs, Preiss, (1975) MTP International Review of Sciences — Biochemistry of Carbohydrates — Biochemistry Series One, 5, pp. 337-389. , 5th edn., W.J. Whelan, Butterworths-University Park Press, London-Baltimore
  • Traut, Lipmann, (1963) J. Biol. Chem., 238, pp. 1213-1221
  • Greenberg, Preiss, (1964) J. Biol. Chem., 239, pp. PC 4314-4315
  • French, (1975) MTP International Review of Sciences — Biochemistry of Carbohydrates — Biochemistry Series One, 5, pp. 267-335. , 5th edn., W.J. Whelan, Butterworths-University Park Press, London-Baltimore
  • Fredrick, (1973) Ann. N.Y. Acad. Sci., 210, pp. 254-264

Citas:

---------- APA ----------
Fontana, J.D. & Krisman, C.R. (1978) . Glycogen synthesis in the fungus Neurospora crassa. BBA - General Subjects, 540(2), 183-189.
http://dx.doi.org/10.1016/0304-4165(78)90130-7
---------- CHICAGO ----------
Fontana, J.D., Krisman, C.R. "Glycogen synthesis in the fungus Neurospora crassa" . BBA - General Subjects 540, no. 2 (1978) : 183-189.
http://dx.doi.org/10.1016/0304-4165(78)90130-7
---------- MLA ----------
Fontana, J.D., Krisman, C.R. "Glycogen synthesis in the fungus Neurospora crassa" . BBA - General Subjects, vol. 540, no. 2, 1978, pp. 183-189.
http://dx.doi.org/10.1016/0304-4165(78)90130-7
---------- VANCOUVER ----------
Fontana, J.D., Krisman, C.R. Glycogen synthesis in the fungus Neurospora crassa. Biochim. Biophys. Acta Gen. Subj. 1978;540(2):183-189.
http://dx.doi.org/10.1016/0304-4165(78)90130-7