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Abstract:

1. 1. Further evidence for the formation of pyrrolic intermediates resulting from action of soybean callus porphobilinogenase on porphobilinogen in the absence or presence of ammonia, under anaerobic conditions, is reported. 2. 2. It was found that the activity of porphobilinogenase was affected by the presence of air; the yield of uroporphyrinogens was very low, although consumption of substrate was not greatly modified. These results suggested that some intermediate in the reaction was still being formed, but that this compound must be in the reduced form to be further converted into uroporphyrinogens. It was observed that ammonia probably produced as accumulation of pyrryl intermediates, but that they synthesize uroporphyrinogen III at a slower rate. 3. 3. Attempts were made to detect the formation of an enzyme-substrate complex, but they all failed. © 1971.

Registro:

Documento: Artículo
Título:Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
Autor:Stella, A.M.; Parera, V.E.; Llambías, E.B.C.; Del C. Batlle, A.M.
Filiación:Cátedra de Química Biológica I, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires Argentina
Palabras clave:ammonia; ammonium sulfate; carbon; hydrolase; isomerase; lyase; oxygen; porphyrin; propionic acid derivative; pyrrole derivative; anaerobic growth; article; enzymology; gel chromatography; kinetics; plant; stereoisomerism; synthesis; thin layer chromatography; Aminohydrolases; Ammonia; Ammonium Sulfate; Anaerobiosis; Carbon Isotopes; Chromatography, Gel; Chromatography, Thin Layer; Isomerases; Kinetics; Lyases; Oxygen; Plants; Porphyrins; Propionates; Pyrroles; Stereoisomerism
Año:1971
Volumen:252
Número:3
Página de inicio:481
Página de fin:488
DOI: http://dx.doi.org/10.1016/0304-4165(71)90151-6
Título revista:BBA - General Subjects
Título revista abreviado:Biochim. Biophys. Acta Gen. Subj.
ISSN:03044165
CODEN:BBGSB
CAS:ammonia, 14798-03-9, 51847-23-5, 7664-41-7; ammonium sulfate, 7783-20-2; carbon, 7440-44-0; hydrolase, 9027-41-2; isomerase, 9013-19-8; lyase, 9055-04-3; oxygen, 7782-44-7; porphyrin, 24869-67-8; Aminohydrolases, EC 3.5.4.; Ammonia, 7664-41-7; Ammonium Sulfate, 7783-20-2; Carbon Isotopes; Isomerases, EC 5.; Lyases, EC 4.; Oxygen, 7782-44-7; Porphyrins; Propionates; Pyrroles
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v252_n3_p481_Stella

Referencias:

  • Lockwood, Rimington, (1957) Biochem. J., 67, p. 8
  • Llambias, del C. Batlle, Studies on the porphobilinogen deaminase-uroporphyrinogen cosynthetase system of cultured soya-bean cells. (1971) Biochem J, 121, p. 327
  • Bogorad, (1960) Comparative Biochemistry of Photoreactive Systems, p. 22. , M.B. Allen, Academic Press, New York
  • Waldenström, Vahlquist, Über ein neues bei der akuten Porphyrie regelmäßig vorkommendes Uroporphyrin. (1935) Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 233, p. 1
  • Llambias, del C. Battle, (1970) FEBS Lett., 6, p. 285
  • Pluscec, Bogorad, (1970) Biochemistry, 9, p. 4736
  • Bogarad, (1958) J. Biol. Chem., 233, p. 501
  • Bogarad, (1958) J. Biol. Chem., 233, p. 510
  • Sancovich, del C. Battle, Grinstein, (1969) Biochimm. Biophys. Acta, 191, p. 130
  • Lockwood, Benzon, (1960) Biochem. J., 75, p. 372
  • Llambias, del C. Battle, Porphyrin biosynthesis. VIII. Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase, its purification, properties and the separation of its components (1971) Biochimica et Biophysica Acta (BBA) - Enzymology, 227, p. 180
  • Bogorad, (1962) Methods in Enzymology, 5, p. 885. , 2nd ed., S.P. Colowick, N.O. Kaplan, Academic Press, New York
  • Bogorad, ENZYMATIC MECHANISMS IN PORPHYRIN SYNTHESIS: POSSIBLE ENZYMATIC BLOCKS IN PORPHYRIAS* (1963) Annals of the New York Academy of Sciences, 104, p. 676
  • Sancovich, del C. Battle, Grinstein, (1969) FEBS Lett., 3, p. 223
  • Sancovich, Ferramola, del C. Battle, Grinstein, (1970) Methods in Enzymology, 17, p. 220. , 2nd ed., S.P. Colowick, N.O. Kaplan, Academic Press, New York
  • Moore, Labbe, (1964) Clin. Chem., 10, p. 1105
  • Rimington, Spectral-absorption coefficients of some porphyrins in the Soret-band region. (1960) Biochem J, 75, p. 620
  • del C. Battle, Grinstein, Porphyrin biosynthesis I. Studies on erythrocyte preparations (1964) Biochimica et Biophysica Acta (BBA) - General Subjects, 82, p. 1
  • del C. Battle, Grinstein, (1962) Lat. Am. Meeting Chem. B.A., p. 107
  • Cornford, Benson, (1963) J. Chromatog., 10, p. 141
  • del C. Battle, Benson, A study of the separation of uroporphyrin octamethly esters I and III by thin-layer and paper chromatography (1966) Journal of Chromatography A, 25, p. 117
  • Llambias, del C. Battle, (1970) Biochim. Biophys. Acta, 220, p. 502
  • Llambias, del C. Battle, (1970) FEBS Lett., 9, p. 180

Citas:

---------- APA ----------
Stella, A.M., Parera, V.E., Llambías, E.B.C. & Del C. Batlle, A.M. (1971) . Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase. BBA - General Subjects, 252(3), 481-488.
http://dx.doi.org/10.1016/0304-4165(71)90151-6
---------- CHICAGO ----------
Stella, A.M., Parera, V.E., Llambías, E.B.C., Del C. Batlle, A.M. "Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase" . BBA - General Subjects 252, no. 3 (1971) : 481-488.
http://dx.doi.org/10.1016/0304-4165(71)90151-6
---------- MLA ----------
Stella, A.M., Parera, V.E., Llambías, E.B.C., Del C. Batlle, A.M. "Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase" . BBA - General Subjects, vol. 252, no. 3, 1971, pp. 481-488.
http://dx.doi.org/10.1016/0304-4165(71)90151-6
---------- VANCOUVER ----------
Stella, A.M., Parera, V.E., Llambías, E.B.C., Del C. Batlle, A.M. Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase. Biochim. Biophys. Acta Gen. Subj. 1971;252(3):481-488.
http://dx.doi.org/10.1016/0304-4165(71)90151-6