Bustamante, J.P.; Bonamore, A.; Nadra, A.D.; Sciamanna, N.; Boffi, A.; Estrin, D.A.; Boechi, L. "Molecular basis of thermal stability in truncated (2/2) hemoglobins" (2014) Biochimica et Biophysica Acta - General Subjects. 1840(7):2281-2288
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Background Understanding the molecular mechanism through which proteins are functional at extreme high and low temperatures is one of the key issues in structural biology. To investigate this phenomenon, we have focused on two instructive truncated hemoglobins from Thermobifida fusca (Tf-trHbO) and Mycobacterium tuberculosis (Mt-trHbO); although the two proteins are structurally nearly identical, only the former is stable at high temperatures. Methods We used molecular dynamics simulations at different temperatures as well as thermal melting profile measurements of both wild type proteins and two mutants designed to interchange the amino acid residue, either Pro or Gly, at E3 position. Results The results show that the presence of a Pro at the E3 position is able to increase (by 8°) or decrease (by 4°) the melting temperature of Mt-trHbO and Tf-trHbO, respectively. We observed that the ProE3 alters the structure of the CD loop, making it more flexible. Conclusions This gain in flexibility allows the protein to concentrate its fluctuations in this single loop and avoid unfolding. The alternate conformations of the CD loop also favor the formation of more salt-bridge interactions, together augmenting the protein's thermostability. General significance These results indicate a clear structural and dynamical role of a key residue for thermal stability in truncated hemoglobins. © 2014 Elsevier B.V.


Documento: Artículo
Título:Molecular basis of thermal stability in truncated (2/2) hemoglobins
Autor:Bustamante, J.P.; Bonamore, A.; Nadra, A.D.; Sciamanna, N.; Boffi, A.; Estrin, D.A.; Boechi, L.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE-CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Biochemical Sciences, Instituto Pasteur, University of Rome la Sapienza, Italy
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Cálculo, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Folding; Molecular dynamics; Mycobacterium tuberculosis; Thermobifida fusca; Thermostability; Truncated hemoglobin; glycine; proline; truncated hemoglobin; amino acid substitution; article; computer simulation; controlled study; hemoglobin analysis; melting point; molecular dynamics; molecular interaction; Mycobacterium tuberculosis; nonhuman; priority journal; protein conformation; protein expression; protein stability; protein structure; protein unfolding; Thermobifida fusca; thermostability; wild type; Folding; Molecular dynamics; Mycobacterium tuberculosis; Thermobifida fusca; Thermostability; Truncated hemoglobin; Actinomycetales; Hot Temperature; Humans; Models, Molecular; Molecular Dynamics Simulation; Mycobacterium tuberculosis; Protein Stability; Truncated Hemoglobins
Página de inicio:2281
Página de fin:2288
Título revista:Biochimica et Biophysica Acta - General Subjects
Título revista abreviado:Biochim. Biophys. Acta Gen. Subj.
CAS:glycine, 56-40-6, 6000-43-7, 6000-44-8; proline, 147-85-3, 7005-20-1


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---------- APA ----------
Bustamante, J.P., Bonamore, A., Nadra, A.D., Sciamanna, N., Boffi, A., Estrin, D.A. & Boechi, L. (2014) . Molecular basis of thermal stability in truncated (2/2) hemoglobins. Biochimica et Biophysica Acta - General Subjects, 1840(7), 2281-2288.
---------- CHICAGO ----------
Bustamante, J.P., Bonamore, A., Nadra, A.D., Sciamanna, N., Boffi, A., Estrin, D.A., et al. "Molecular basis of thermal stability in truncated (2/2) hemoglobins" . Biochimica et Biophysica Acta - General Subjects 1840, no. 7 (2014) : 2281-2288.
---------- MLA ----------
Bustamante, J.P., Bonamore, A., Nadra, A.D., Sciamanna, N., Boffi, A., Estrin, D.A., et al. "Molecular basis of thermal stability in truncated (2/2) hemoglobins" . Biochimica et Biophysica Acta - General Subjects, vol. 1840, no. 7, 2014, pp. 2281-2288.
---------- VANCOUVER ----------
Bustamante, J.P., Bonamore, A., Nadra, A.D., Sciamanna, N., Boffi, A., Estrin, D.A., et al. Molecular basis of thermal stability in truncated (2/2) hemoglobins. Biochim. Biophys. Acta Gen. Subj. 2014;1840(7):2281-2288.