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Leal Denis, M.F.; Incicco, J.J.; Espelt, M.V.; Verstraeten, S.V.; Pignataro, O.P.; Lazarowski, E.R.; Schwarzbaum, P.J. "Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes" (2013) Biochimica et Biophysica Acta - General Subjects. 1830(10):4692-4707
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Abstract:

Background The peptide mastoparan 7 (MST7) stimulated ATP release in human erythrocytes. We explored intra- and extracellular processes governing the time-dependent accumulation of extracellular ATP (i.e., ATPe kinetics). Methods Human erythrocytes were treated with MST7 in the presence or absence of two blockers of pannexin 1. ATPe concentration was monitored by luciferin-luciferase based real-time luminometry. Results Exposure of human erythrocytes to MST7 led to an acute increase in [ATPe], followed by a slower increase phase. ATPe kinetics reflected a strong activation of ATP efflux and a low rate of ATPe hydrolysis by ectoATPase activity. Enhancement of [ATPe] by MST7 required adhesion of erythrocytes to poly-D-lysin-coated coverslips, and correlated with a 31% increase of cAMP and 10% cell swelling. However, when MST7 was dissolved in a hyperosmotic medium to block cell swelling, ATPe accumulation was inhibited by 49%. Erythrocytes pre-exposure to 10 μM of either carbenoxolone or probenecid, two blockers of pannexin 1, exhibited a partial reduction of ATP efflux. Erythrocytes from pannexin 1 knockout mice exhibited similar ATPe kinetics as those of wild type mice erythrocytes exposed to pannexin 1 blockers. Conclusions MST7 induced release of ATP required either cell adhesion or strong activation of cAMP synthesis. Part of this release required cell swelling. Kinetic analysis and a data driven model suggested that ATP efflux is mediated by two ATP conduits displaying different kinetics, with one conduit being fully blocked by pannexin 1 blockers. General significance Kinetic analysis of extracellular ATP accumulation from human erythrocytes and potential effects on microcirculation. © 2013 Elsevier B.V.

Registro:

Documento: Artículo
Título:Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes
Autor:Leal Denis, M.F.; Incicco, J.J.; Espelt, M.V.; Verstraeten, S.V.; Pignataro, O.P.; Lazarowski, E.R.; Schwarzbaum, P.J.
Filiación:Department of Biological Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
Laboratory of Molecular Endocrinology and Signal Transduction, Institute of Biology and Experimental Medicine-CONICET, Vuelta de Obligado 2490, CP 1428 Buenos Aires, Argentina
Department of Biological Chemistry, School of Sciences, University of Buenos Aires, Buenos Aires, Argentina
Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, 4029A Thurston Bowles Building, Chapel Hill, NC 27599-7248, United States
Palabras clave:ATP; ATPases; Erythrocyte; Extracellular; Pannexin 1; adenosine triphosphatase; adenosine triphosphate; carbenoxolone; cell protein; mastoparan; mastoparan 7; pannexin 1; poly dextro lysine; probenecid; unclassified drug; animal cell; article; cell activation; cell swelling; chemical reaction kinetics; concentration (parameters); controlled study; enzyme activity; erythrocyte; erythrocyte adhesiveness; erythrocyte volume; experimental dog; hematocrit; human; human cell; hydrolysis; inhibition kinetics; intracellular signaling; long term exposure; mouse; nonhuman; nucleotide transport; priority journal; reduction kinetics; wild type; 3V; a cAMP activating cocktail containing isoproterenol, forskolin and papaverine; ATPases; ATPe; ATPi; carbenoxolone; CBX; cilostazol; CTZ; Erythrocyte; extracellular ATP; Extracellular ATP; flux of ATP release; flux of ATPe hydrolysis; GEFs; guanine exchange factors; intracellular ATP; J(R); J(V); mastoparan; MST; pannexin 1; Pannexin 1; pannexin 1 heterozygous mice; pannexin 1 knockout mice, PTX, pertussis toxin; pannexin 1 wild type mice; PBC; pnx; pnx(+/+); pnx(+/-); pnx(-/-); probenecid; rbcs; red blood cells; relative cell volume; Vr; Adenosine Triphosphate; Animals; Dogs; Erythrocytes; Humans; Hydrolysis; Kinetics; Mice; Mice, Knockout; Peptides; Signal Transduction; Mus
Año:2013
Volumen:1830
Número:10
Página de inicio:4692
Página de fin:4707
DOI: http://dx.doi.org/10.1016/j.bbagen.2013.05.033
Título revista:Biochimica et Biophysica Acta - General Subjects
Título revista abreviado:Biochim. Biophys. Acta Gen. Subj.
ISSN:03044165
CODEN:BBGSB
CAS:adenosine triphosphatase, 37289-25-1, 9000-83-3; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; carbenoxolone, 5697-56-3, 7421-40-1; mastoparan, 72093-21-1; probenecid, 57-66-9; Adenosine Triphosphate, 56-65-5; Mas7 protein, synthetic; Peptides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1830_n10_p4692_LealDenis

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Citas:

---------- APA ----------
Leal Denis, M.F., Incicco, J.J., Espelt, M.V., Verstraeten, S.V., Pignataro, O.P., Lazarowski, E.R. & Schwarzbaum, P.J. (2013) . Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes. Biochimica et Biophysica Acta - General Subjects, 1830(10), 4692-4707.
http://dx.doi.org/10.1016/j.bbagen.2013.05.033
---------- CHICAGO ----------
Leal Denis, M.F., Incicco, J.J., Espelt, M.V., Verstraeten, S.V., Pignataro, O.P., Lazarowski, E.R., et al. "Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes" . Biochimica et Biophysica Acta - General Subjects 1830, no. 10 (2013) : 4692-4707.
http://dx.doi.org/10.1016/j.bbagen.2013.05.033
---------- MLA ----------
Leal Denis, M.F., Incicco, J.J., Espelt, M.V., Verstraeten, S.V., Pignataro, O.P., Lazarowski, E.R., et al. "Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes" . Biochimica et Biophysica Acta - General Subjects, vol. 1830, no. 10, 2013, pp. 4692-4707.
http://dx.doi.org/10.1016/j.bbagen.2013.05.033
---------- VANCOUVER ----------
Leal Denis, M.F., Incicco, J.J., Espelt, M.V., Verstraeten, S.V., Pignataro, O.P., Lazarowski, E.R., et al. Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes. Biochim. Biophys. Acta Gen. Subj. 2013;1830(10):4692-4707.
http://dx.doi.org/10.1016/j.bbagen.2013.05.033