Artículo

Dasso, M.; Pagotto, R.; Pignataro, O.P.; Diez, R.A.; Sales, M.E. "Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms" (2011) Biochimica et Biophysica Acta - General Subjects. 1810(12):1212-1219
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Abstract:

Background: Amylase is synthesized in submandibular glands (SMG) and released into the oral cavity to degrade carbohydrates in the mouth. Bitter taste receptors (T2R) belong to the G-protein coupled receptor (GPCR) family and are expressed in the taste cells and also in the digestive tract. Methods: The activity of amylase secreted by murine SMG was measured, detecting maltose by Bernfeld's method. Amylase and T2R6 were detected by imunohistochemistry and Western blot. The expression of Ggustducin, Gi, and phospholipase Cβ2 was also studied by Western blot. cAMP levels were measured by radioimmunoassay and inositol monophosphate production was quantified by ELISA. Results: Theophylline, denatonium and cycloheximide exerted a dose-dependent inhibition on amylase secretion. This effect was reverted by preincubating SMG with an anti-Gαi antibody. cAMP production was increased by the same compounds, an effect that was also abrogated by an anti-Gαi antibody. Bitter compounds reduced inositol monophosphate formation in SMG and H-89, a protein kinase A inhibitor, reverted this action, revealing that this protein kinase down regulates phospholipase C activity. General significance: We demonstrated that theophylline, denatonium and cycloheximide inhibit salivary amylase secretion, activating an intracellular signaling pathway that involves cAMP and phospholipase C, that cross talks via protein kinase A. © 2011 Elsevier B.V.

Registro:

Documento: Artículo
Título:Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
Autor:Dasso, M.; Pagotto, R.; Pignataro, O.P.; Diez, R.A.; Sales, M.E.
Filiación:Centro de Estudios Farmacológicos y Botánicos (CEFYBO)-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 piso 16 sector der. CP 1121, Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental (IBYME)-CONICET, Buenos Aires, Argentina
Dpto. de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Cátedra de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Amylase; Bitter agonist; cAMP; Gi protein; Inositol monophosphate; Protein kinase A; amylase; cyclic AMP; cyclic AMP dependent protein kinase; cycloheximide; denatonium benzoate; guanine nucleotide binding protein; gustducin; inhibitory guanine nucleotide binding protein; inositol phosphate; phospholipase C beta2; theophylline; unclassified drug; animal tissue; article; bitter taste; controlled study; enzyme activity; enzyme inhibition; enzyme linked immunosorbent assay; enzyme localization; enzyme release; immunohistochemistry; mouse; nonhuman; priority journal; protein expression; protein function; quantitative analysis; radioimmunoassay; signal transduction; submandibular gland; taste bud; Western blotting; Amylases; Animals; Blotting, Western; Cyclic AMP; Immunohistochemistry; Mice; Mice, Inbred BALB C; Signal Transduction; Submandibular Gland; Murinae
Año:2011
Volumen:1810
Número:12
Página de inicio:1212
Página de fin:1219
DOI: http://dx.doi.org/10.1016/j.bbagen.2011.08.009
Título revista:Biochimica et Biophysica Acta - General Subjects
Título revista abreviado:Biochim. Biophys. Acta Gen. Subj.
ISSN:03044165
CODEN:BBGSB
CAS:amylase, 9000-90-2, 9000-92-4, 9001-19-8; cyclic AMP, 60-92-4; cycloheximide, 642-81-9, 66-81-9; denatonium benzoate, 3734-33-6; inositol phosphate, 15421-51-9; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; Amylases, 3.2.1.-; Cyclic AMP, 60-92-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1810_n12_p1212_Dasso

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Citas:

---------- APA ----------
Dasso, M., Pagotto, R., Pignataro, O.P., Diez, R.A. & Sales, M.E. (2011) . Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms. Biochimica et Biophysica Acta - General Subjects, 1810(12), 1212-1219.
http://dx.doi.org/10.1016/j.bbagen.2011.08.009
---------- CHICAGO ----------
Dasso, M., Pagotto, R., Pignataro, O.P., Diez, R.A., Sales, M.E. "Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms" . Biochimica et Biophysica Acta - General Subjects 1810, no. 12 (2011) : 1212-1219.
http://dx.doi.org/10.1016/j.bbagen.2011.08.009
---------- MLA ----------
Dasso, M., Pagotto, R., Pignataro, O.P., Diez, R.A., Sales, M.E. "Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms" . Biochimica et Biophysica Acta - General Subjects, vol. 1810, no. 12, 2011, pp. 1212-1219.
http://dx.doi.org/10.1016/j.bbagen.2011.08.009
---------- VANCOUVER ----------
Dasso, M., Pagotto, R., Pignataro, O.P., Diez, R.A., Sales, M.E. Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms. Biochim. Biophys. Acta Gen. Subj. 2011;1810(12):1212-1219.
http://dx.doi.org/10.1016/j.bbagen.2011.08.009