Artículo

Carrillo, C.; Serra, M.P.; Pereira, C.A.; Huber, A.; González, N.S.; Algranati, I.D. "Heterologous expression of a plant arginine decarboxylase gene in Trypanosoma cruzi" (2004) Biochimica et Biophysica Acta - General Subjects. 1674(3):223-230
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Abstract:

Wild-type Trypanosoma cruzi epimastigotes lack arginine decarboxylase (ADC) enzymatic activity. However, the transformation of these parasites with a recombinant plasmid containing the oat ADC cDNA coding region gave rise to the transient heterologous expression of the enzyme, suggesting the absence of endogenous mechanisms that could inhibit the expression of a hypothetical own ADC gene or the assay used to measure its enzymatic activity. The foreign ADC enzyme expressed in the transgenic T. cruzi was characterized by identification of the products, the stoichiometry of the catalysed reaction, the specific inhibition by α-difluoromethylarginine (DFMA) and the study of its metabolic turnover. The half-life of the heterologous ADC activity in T. cruzi was about 150 min. Bioinformatics studies and polymerase chain reaction (PCR) analyses seem to indicate the absence of ADC-like DNA sequences in the wild-type T. cruzi genome. © 2004 Published by Elsevier B.V.

Registro:

Documento: Artículo
Título:Heterologous expression of a plant arginine decarboxylase gene in Trypanosoma cruzi
Autor:Carrillo, C.; Serra, M.P.; Pereira, C.A.; Huber, A.; González, N.S.; Algranati, I.D.
Filiación:Fundación Instituto Leloir, Fac. de Ciencias Exactas Y Naturales, Univ. Buenos Aires CONICET, A. M., Argentina
Inst. Invest. Medicas Alfredo Lanari, UBA and CONICET, Combatientes Malvinas 3150, 1427 B., Argentina
Palabras clave:Agmatine; Arginine decarboxylase; Polyamine biosynthesis; Transgenic parasite; Trypanosoma cruzi epimastigote; alpha difluoromethylarginine; arginine decarboxylase gene; article; bioinformatics; biolistic transformation; catalysis; catalyst; controlled study; DNA sequence; enzyme activity; enzyme assay; enzyme inhibition; gene expression; genetic code; genetic recombination; genome; half life time; heterologous expression; intermethod comparison; metabolic regulation; nonhuman; plant; polymerase chain reaction; priority journal; reaction analysis; sequence analysis; stoichiometry; transgene; Trypanosoma cruzi; wild type; Amino Acid Sequence; Animals; Animals, Genetically Modified; Avena sativa; Base Sequence; Carboxy-Lyases; DNA Primers; DNA, Complementary; Enzyme Inhibitors; Molecular Sequence Data; Open Reading Frames; Recombinant Proteins; Transfection; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi
Año:2004
Volumen:1674
Número:3
Página de inicio:223
Página de fin:230
DOI: http://dx.doi.org/10.1016/j.bbagen.2004.06.017
Título revista:Biochimica et Biophysica Acta - General Subjects
Título revista abreviado:Biochim. Biophys. Acta Gen. Subj.
ISSN:03044165
CODEN:BBGSB
CAS:alpha difluoromethylarginine, 69955-43-7; Carboxy-Lyases, 4.1.1.-; DNA Primers; DNA, Complementary; Enzyme Inhibitors; Recombinant Proteins; arginine decarboxylase, 4.1.1.19
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1674_n3_p223_Carrillo

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Citas:

---------- APA ----------
Carrillo, C., Serra, M.P., Pereira, C.A., Huber, A., González, N.S. & Algranati, I.D. (2004) . Heterologous expression of a plant arginine decarboxylase gene in Trypanosoma cruzi. Biochimica et Biophysica Acta - General Subjects, 1674(3), 223-230.
http://dx.doi.org/10.1016/j.bbagen.2004.06.017
---------- CHICAGO ----------
Carrillo, C., Serra, M.P., Pereira, C.A., Huber, A., González, N.S., Algranati, I.D. "Heterologous expression of a plant arginine decarboxylase gene in Trypanosoma cruzi" . Biochimica et Biophysica Acta - General Subjects 1674, no. 3 (2004) : 223-230.
http://dx.doi.org/10.1016/j.bbagen.2004.06.017
---------- MLA ----------
Carrillo, C., Serra, M.P., Pereira, C.A., Huber, A., González, N.S., Algranati, I.D. "Heterologous expression of a plant arginine decarboxylase gene in Trypanosoma cruzi" . Biochimica et Biophysica Acta - General Subjects, vol. 1674, no. 3, 2004, pp. 223-230.
http://dx.doi.org/10.1016/j.bbagen.2004.06.017
---------- VANCOUVER ----------
Carrillo, C., Serra, M.P., Pereira, C.A., Huber, A., González, N.S., Algranati, I.D. Heterologous expression of a plant arginine decarboxylase gene in Trypanosoma cruzi. Biochim. Biophys. Acta Gen. Subj. 2004;1674(3):223-230.
http://dx.doi.org/10.1016/j.bbagen.2004.06.017