1. 1. The role of 5- and 6-COOH porphyrinogens III in haem biosynthesis was studied in vitro. 2. 2. When haemolysates of normal chicken erythrocytes were incubated in the presence of 5- or 6-COOH porphyrinogens III, a marked isotopic dilution was observed served on the [2-12C]glycine incorporation into haem. This effect was similar and even greater than that produced by uroporphyrinogen III and coproporphyrinogen III. To confirm these results 14C-labelled porphyrinogens of 5- and 6-COOH were incubated with chicken erythrocyte haemolysates. Labelling of the haem took place in a way similar to that when [14C]uroporphyrinogen III was used. 3. 3. These results are consistent with the idea that 5-COOH porphyrinogen III and 6-COOH porphyrinogen III are normal intermediates in haem biosynthesis with a stepwise decarboxylation process of uroporphyrinogen III to coproporphyrinogen III according to the following scheme: Uroporphyrinogen III → 7-COOH porphyrinogen III → 6-COOH porphyrinogen III → 5-COOH porphyrinogen III → coproporphyrinogen III. © 1968.
Documento: | Artículo |
Título: | Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis |
Autor: | San Martín De Viale, L.C.; Grinstein, M.; Grinstein, M. |
Filiación: | Departamento de Química Biológica, Orientación Química Biológica I. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina |
Palabras clave: | carbon; glycine; heme; porphyrin; animal; article; biosynthesis; chicken; enzymology; erythrocyte; female; hemolysis; male; metabolism; rat; urine; Animal; Carbon Isotopes; Chickens; Erythrocytes; Female; Glycine; Heme; Hemolysis; Male; Porphyrins; Rats |
Año: | 1968 |
Volumen: | 158 |
Número: | 1 |
Página de inicio: | 79 |
Página de fin: | 91 |
Título revista: | BBA - General Subjects |
Título revista abreviado: | Biochim. Biophys. Acta Gen. Subj. |
ISSN: | 03044165 |
CODEN: | BBGSB |
CAS: | carbon, 7440-44-0; glycine, 56-40-6, 6000-43-7, 6000-44-8; heme, 14875-96-8; porphyrin, 24869-67-8; Carbon Isotopes; Glycine, 56-40-6; Heme, 14875-96-8; Porphyrins |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v158_n1_p79_SanMartinDeViale |