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Abstract:

Fasciolosis, caused by the trematode Fasciola hepatica, is a zoonosis of economic importance in livestock that is emerging as a chronic disease in humans. The intermediate hosts are lymnaeid snails, in which diagnosis of infection is traditionally based on cercarial shedding, tissue sectioning and crushing. We developed a PCR assay for the sensitive and specific detection of F. hepatica in field-collected Lymnaea sp. snails. A primer pair was designed to amplify a 405 bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica. The PCR assay showed a limit of detection of 10 pg of genomic F. hepatica DNA. No cross-reactions were observed with samples from other related trematode species or from the snail hosts Lymnaea columella and Lymnaea viatrix. DNA sequencing of the amplicon showed 100% homology with F. hepatica, and 75-89% homology with other trematodes on regions that did not include the entire set of primers. Two samples from Argentina were analysed. For snails in sample 1 (n = 240), identified as L. columella, the infection rate was 17.5 and 51.3% by direct examination and PCR, respectively. For snails in sample 2 (n = 34), identified as L. viatrix, the infection rate was 2.9 and 61.8% by direct examination and PCR, respectively. Differences in infection rates between these diagnosis methods were significant for both samples. Our PCR technique showed to be effective for detecting specific F. hepatica infections of low intensity in the intermediate host, and hence it could be used to study the epidemiological situation in a given area, as well as to assess host suitability for the parasite. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails
Autor:Cucher, M.A.; Carnevale, S.; Prepelitchi, L.; Labbé, J.H.; Wisnivesky-Colli, C.
Filiación:Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr. Carlos G. Malbrán, Av. Velez Sarsfield 563, 1281 Buenos Aires, Argentina
Unidad de Ecología de Reservorios Y Vectores de Parásitos, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Fasciola hepatica; Lymnaea sp.; PCR; cytochrome c oxidase; amplicon; article; controlled study; cross reaction; DNA sequence; Fasciola hepatica; infection rate; Lymnaea; lymnaea columella; Lymnaea viatrix; nonhuman; nucleotide sequence; polymerase chain reaction; sensitivity and specificity; snail; Animals; Argentina; Base Sequence; Cross Reactions; Disease Reservoirs; Disease Vectors; DNA Primers; Electron Transport Complex IV; Fasciola hepatica; Fascioliasis; Lymnaea; Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Alignment; Sequence Homology, Nucleic Acid; Species Specificity; Columella; Fasciola hepatica; Gastropoda; Lymnaea; Lymnaea columella; Lymnaea viatrix; Lymnaeidae; Trematoda; Viatrix
Año:2006
Volumen:137
Número:1-2
Página de inicio:74
Página de fin:82
DOI: http://dx.doi.org/10.1016/j.vetpar.2005.12.013
Título revista:Veterinary Parasitology
Título revista abreviado:Vet. Parasitol.
ISSN:03044017
CODEN:VPARD
CAS:cytochrome c oxidase, 72841-18-0, 9001-16-5; DNA Primers; Electron Transport Complex IV, EC 1.9.3.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044017_v137_n1-2_p74_Cucher

Referencias:

  • Castellanos, Z., Landoni, N.A., Mollusca, Gasteropoda, Lymnaeidae (1981) Fauna de Agua Dulce de la República Argentina, Vol. XV, , FECIC Buenos Aires
  • Cohen, J., A coefficient of agreement for nominal scales (1960) Educ. Psychol. Meas., 20, pp. 37-46
  • Hamburger, J., Hoffman, O., Kariuki, H.C., Muchiri, E.M., Ouma, J.H., Koech, D.K., Sturrock, R.F., King, C.H., Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: A new tool for studying the dynamics of snail infection (2004) Am. J. Trop. Med. Hyg., 71, pp. 765-773
  • Hopkins, D.R., Homing in on helminths (1992) Am. J. Trop. Med. Hyg., 46, pp. 626-634
  • Kamenetzky, L., Canova, S.G., Guarnera, E., Rosenzvit, M., Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts (2000) Exp. Parasitol., 95, pp. 122-127
  • Kaplan, R.M., Dame, J.B., Reddy, G.R., Courtney, C.H., A repetitive DNA probe for the sensitive detection of Fasciola hepatica infected snails (1995) Int. J. Parasitol., 25, pp. 601-610
  • Kaplan, R.M., Dame, J.B., Reddy, G.R., Courtney, C.H., The prevalence of Fasciola hepatica in its snail intermediate host determined by DNA probe assay (1997) Int. J. Parasitol., 27, pp. 1585-1593
  • Khallaayoune, K.H., Stromberg, B.E., Dakkak, A., Malone, J.B., Seasonal dynamics of Fasciola hepatica burdens in grazing timahdit sheep in Morocco (1991) Int. J. Parasitol., 21, pp. 307-314
  • Kleiman, F., Pietrokovsky, S., Paraense, W.L., Wisnivesky-Colli, M.C., Southernmost finding of Lymnaea viatrix Orbingy, 1835 (Pulmonata: Lymnaeidae), intermediate host of Fasciola hepatica (Linnaeus, 1758) (Trematoda: Digenea), in urban and rural areas of Patagonia, Argentina (2004) Mem. Inst. Oswaldo Cruz, 99, pp. 23-24
  • Lombardero, O., Moriena, R., Racioppi, O., Quiroz, L., Bionomía de Lymnaea sp. en la provincia de Corrientes (1979) Veterinaria (Corrientes, Argentina), 2, pp. 5-20
  • Magalhaes, K.G., Konovaloff, J.P., Dos Santos Carvalho, O., Detection of Lymnaea columella infection by Fasciola hepatica through multiplex-PCR (2004) Mem. Inst. Oswaldo Cruz, 99, pp. 421-424
  • Maniatis, T., Fritsch, E.F., Sambrook, J., (1989) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
  • Mas-Coma, S., Funatsu, I.R., Bargues, M.D., Fasciola hepatica and lymnaeid snails occurring at very high altitude in South America (2001) Parasitology, 123, pp. 115-127
  • Olsen, O.W., Bionomics of the lymnaeid snail, Stagnicola bulimoides techella, the intermediate host of the liver fluke in Southern Texas (1944) J. Agric. Res., 69, pp. 389-403
  • Paraense, W.L., Lymnaea viatrix: A study of topotypic specimens (Mollusca: Lymnaeidae) (1976) Rev. Bras. Biol., 36, pp. 419-428
  • Paraense, W.L., Lymnaea viatrix and Lymnaea columella in the neotropical region: A distributional outline (1982) Mem. Inst. Oswaldo Cruz, 77, pp. 181-188
  • Paraense, W.L., Lymnaea diaphana: A study of topotypic specimens (Pulmonata: Lymnaeaidae) (1984) Mem. Inst. Oswaldo Cruz, 79, pp. 75-81
  • Prepelitchi, L., Kleiman, F., Pietrokovsky, S., Moriena, R.A., Racioppi, O., Álvarez, J., Wisnivesky-Colli, M.C., First report of Lymnaea columella Say, 1817 (Pulmonata: Lymnaeidae) naturally infected with Fasciola hepatica (Linnaeus, 1758) (Trematoda: Digenea) in Argentina (2003) Mem. Inst. Oswaldo Cruz, 98, pp. 889-891
  • Rim, H.J., Farag, H.F., Sornmani, S., Cross, J.H., Food-borne trematodes: Ignored or emerging? (1994) Parasitol. Today, 10, pp. 207-209
  • Rognlie, M.C., Dimke, K.L., Knapp, S.E., Detection of Fasciola hepatica in infected intermediate hosts using RT-PCR (1994) J. Parasitol., 80, pp. 748-755
  • Rognlie, M.C., Dimke, K.L., Potts, R.S., Knapp, S.E., Seasonal transmission of Fasciola hepatica hosts using a DNA-based assay (1996) Vet. Parasitol., 65, pp. 297-305
  • Rossanigo, C.E., Avila, J.D., Vásquez, R., Sager, L., Incidencia, distribución e identificación del huésped intermediario de la distomatosis bovina en la provincia de San Luis (1983) Gaceta Veterinaria XLV, 382, pp. 739-746
  • Rubel, D., Prepelitchi, L., Kleiman, F., Carnevale, S., Wisnivesky-Colli, C., Estudio de foco a partir de un caso humano de fasciolosis en la localidad de Loncopué, Neuquén, Argentina (2005) Medicina (B Aires), 65, pp. 207-212
  • Shubkin, C.D., White, M.W., Abrahamsen, M.S., Rognlie, M.C., Knapp, S.E., A nucleic acid-based test for detection of Fasciola hepatica (1992) J. Parasitol., 78, pp. 817-821
  • Van Der Knaap, W.P.W., Loker, E.S., Immune mechanisms in trematode-snail interactions (1990) Parasitol. Today, 6, pp. 175-182
  • Venturini, L., Fonrouge, R., Lymnaea viator (D́Orbingy 1835): Aspectos de su biología en condiciones naturales (1985) Rev. Med. Vet. (Bs. As.), 66, pp. 340-345

Citas:

---------- APA ----------
Cucher, M.A., Carnevale, S., Prepelitchi, L., Labbé, J.H. & Wisnivesky-Colli, C. (2006) . PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails. Veterinary Parasitology, 137(1-2), 74-82.
http://dx.doi.org/10.1016/j.vetpar.2005.12.013
---------- CHICAGO ----------
Cucher, M.A., Carnevale, S., Prepelitchi, L., Labbé, J.H., Wisnivesky-Colli, C. "PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails" . Veterinary Parasitology 137, no. 1-2 (2006) : 74-82.
http://dx.doi.org/10.1016/j.vetpar.2005.12.013
---------- MLA ----------
Cucher, M.A., Carnevale, S., Prepelitchi, L., Labbé, J.H., Wisnivesky-Colli, C. "PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails" . Veterinary Parasitology, vol. 137, no. 1-2, 2006, pp. 74-82.
http://dx.doi.org/10.1016/j.vetpar.2005.12.013
---------- VANCOUVER ----------
Cucher, M.A., Carnevale, S., Prepelitchi, L., Labbé, J.H., Wisnivesky-Colli, C. PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails. Vet. Parasitol. 2006;137(1-2):74-82.
http://dx.doi.org/10.1016/j.vetpar.2005.12.013