Artículo
Lipovsek, M.; Plazas, P.; Savino, J.; Klaassen, A.; Boulter, J.; Elgoyhen, A.B.; Katz, E. "Properties of mutated murine α4β2 nicotinic receptors linked to partial epilepsy" (2008) Neuroscience Letters. 434(2):165-169
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Abstract:
We characterized, by electrophysiological methods, two biophysical properties of murine recombinant α4β2 nicotinic acetylcholine receptors (nAChR) bearing a mutation (α4:+L264α4:β2 or α4:S252Fα4:β2) linked to autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE). Sensitivity to acetylcholine (ACh) was increased by the S252F substitution expressed in heterozygosis (α4:S252Fα4:β2) but was markedly reduced when this mutation was expressed in homozygosis (S252Fα4:β2). ACh sensitivity was not altered by the +L264 insertion. Moreover, receptor desensitization was significantly increased by both mutations expressed in heterozygosis. These results are in general agreement to those of rat and human recombinant receptors bearing the same mutations, thus contributing to validate the use of knock-in mice harboring ADNFLE mutations as models to study this pathology. © 2008 Elsevier Ireland Ltd. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Properties of mutated murine α4β2 nicotinic receptors linked to partial epilepsy |
| Autor: | Lipovsek, M.; Plazas, P.; Savino, J.; Klaassen, A.; Boulter, J.; Elgoyhen, A.B.; Katz, E. |
| Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina Department of Psychiatry and Behavioral Sciences, Hatos Research Center for Neuropharmacology, Brain Research and Molecular Biology Institutes, University of California, Los Angeles, CA 9005, United States Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires, 1121, Argentina Dpto. de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina |
| Palabras clave: | ACh; Desensitization; Epilepsy; Ion channels; Nicotinic receptors; acetylcholine; alpha4beta2 nicotinic receptor; nicotinic receptor; unclassified drug; animal cell; article; autosomal dominant disorder; controlled study; electrophysiology; focal epilepsy; frontal lobe epilepsy; gene insertion; gene mutation; heterozygosity; homozygosity; nonhuman; nucleotide sequence; priority journal; receptor down regulation; Xenopus; Acetylcholine; Animals; Cholinergic Agents; Epilepsies, Partial; Genes, Dominant; Heterozygote; Homozygote; Ion Channel Gating; Mice; Models, Chemical; Mutagenesis; Oocytes; Patch-Clamp Techniques; Receptors, Nicotinic; Xenopus |
| Año: | 2008 |
| Volumen: | 434 |
| Número: | 2 |
| Página de inicio: | 165 |
| Página de fin: | 169 |
| DOI: | http://dx.doi.org/10.1016/j.neulet.2007.12.061 |
| Título revista: | Neuroscience Letters |
| Título revista abreviado: | Neurosci. Lett. |
| ISSN: | 03043940 |
| CODEN: | NELED |
| CAS: | acetylcholine, 51-84-3, 60-31-1, 66-23-9; Acetylcholine, 51-84-3; Cholinergic Agents; Receptors, Nicotinic; nicotinic receptor alpha4beta2 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v434_n2_p165_Lipovsek |
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Citas:
---------- APA ----------
Lipovsek, M., Plazas, P., Savino, J., Klaassen, A., Boulter, J., Elgoyhen, A.B. & Katz, E. (2008) . Properties of mutated murine α4β2 nicotinic receptors linked to partial epilepsy. Neuroscience Letters, 434(2), 165-169.http://dx.doi.org/10.1016/j.neulet.2007.12.061
---------- CHICAGO ----------
Lipovsek, M., Plazas, P., Savino, J., Klaassen, A., Boulter, J., Elgoyhen, A.B., et al. "Properties of mutated murine α4β2 nicotinic receptors linked to partial epilepsy" . Neuroscience Letters 434, no. 2 (2008) : 165-169.http://dx.doi.org/10.1016/j.neulet.2007.12.061
---------- MLA ----------
Lipovsek, M., Plazas, P., Savino, J., Klaassen, A., Boulter, J., Elgoyhen, A.B., et al. "Properties of mutated murine α4β2 nicotinic receptors linked to partial epilepsy" . Neuroscience Letters, vol. 434, no. 2, 2008, pp. 165-169.http://dx.doi.org/10.1016/j.neulet.2007.12.061
---------- VANCOUVER ----------
Lipovsek, M., Plazas, P., Savino, J., Klaassen, A., Boulter, J., Elgoyhen, A.B., et al. Properties of mutated murine α4β2 nicotinic receptors linked to partial epilepsy. Neurosci. Lett. 2008;434(2):165-169.http://dx.doi.org/10.1016/j.neulet.2007.12.061
