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Abstract:

N-β-Alanyldopamine (NBAD) is the primary catechol tanning agent precursor in typical brown or yellow insect cuticle. The insect integument enzyme responsible for the synthesis of NBAD was reported to be expressed solely in the epidermis, and only at the time of cuticle sclerotization. However, in this study we demonstrate directly that the enzyme also is expressed in a constitutive manner in the neural system of insects. The requirements and kinetic parameters of the brain-associated enzyme appear similar to those of the epidermis-associated enzyme in Ceratitis capitata. The brain-associated enzyme also was able to catalyze the in vitro synthesis of N-β- alanylnorepinephrine (NBANE) and β-alanyl derivatives of other biogenic amines. A melanic mutant of C. capitata, niger, was unable to conjugate β-alanine with dopamine or other amines in either the epidermis or the brain. This result strongly supports the idea that these enzymes actually are expressed from a single gene and that differences in regulation must exist that account for the constitutive expression in the neural system. Similar results were obtained in Drosophila melanogaster and other insects. From these data, a number of questions arise about the role of β-alanyl derivatives of biogenic amines and other compounds in insect brain and similarly, in the mammalian CNS. © 2004 Elsevier Ireland Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain
Autor:Pérez, M.; Schachter, J.; Quesada-Allué, L.A.
Filiación:Inst. de Invest. Bioquímicas, FCEyN-Univ. Buenos Aires and CONICET, Patricias Argentinas 435, Buenos A., Argentina
Palabras clave:β-Alanine; Biogenic amines; Ceratitis capitata; Medfly; N-β-Alanyldopamine; N-β-Alanylnorepinephrine; brain amine; catecholamine; ligase; n beta alanylcatecholamine ligase; n beta alanylnorepinephrine; noradrenalin; unclassified drug; animal tissue; article; catalysis; central nervous system; controlled study; Drosophila melanogaster; enzyme activity; enzyme synthesis; epidermis; gene control; gene mutation; kinetics; Mediterranean fruit fly; nonhuman; parameter; priority journal; protein expression; protein function; Animals; Brain; Brain Chemistry; Catecholamines; Cell Extracts; Chromatography, High Pressure Liquid; Diptera; Dopamine; Insect Proteins; Ligases
Año:2004
Volumen:368
Número:2
Página de inicio:186
Página de fin:191
DOI: http://dx.doi.org/10.1016/j.neulet.2004.07.015
Título revista:Neuroscience Letters
Título revista abreviado:Neurosci. Lett.
ISSN:03043940
CODEN:NELED
CAS:ligase, 9080-13-1; noradrenalin, 1407-84-7, 51-41-2; Catecholamines; Cell Extracts; Dopamine, 51-61-6; Insect Proteins; Ligases, 6.-; N(beta)-alanyldopamine, 54653-62-2; catecholamine-beta-alanyl ligase, 6.3.2.; cuticle proteins, insects
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v368_n2_p186_Perez

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Citas:

---------- APA ----------
Pérez, M., Schachter, J. & Quesada-Allué, L.A. (2004) . Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain. Neuroscience Letters, 368(2), 186-191.
http://dx.doi.org/10.1016/j.neulet.2004.07.015
---------- CHICAGO ----------
Pérez, M., Schachter, J., Quesada-Allué, L.A. "Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain" . Neuroscience Letters 368, no. 2 (2004) : 186-191.
http://dx.doi.org/10.1016/j.neulet.2004.07.015
---------- MLA ----------
Pérez, M., Schachter, J., Quesada-Allué, L.A. "Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain" . Neuroscience Letters, vol. 368, no. 2, 2004, pp. 186-191.
http://dx.doi.org/10.1016/j.neulet.2004.07.015
---------- VANCOUVER ----------
Pérez, M., Schachter, J., Quesada-Allué, L.A. Constitutive activity of N-β-alanyl-catecholamine ligase in insect brain. Neurosci. Lett. 2004;368(2):186-191.
http://dx.doi.org/10.1016/j.neulet.2004.07.015