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Abstract:

Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by nitric oxide (NO), we studied if deoxycorticosterone acetate (DOCA) treatment changed the number of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) active neurons and neuronal NO synthase (nNOS)-immunoreactive (IR) cells in the PVN and SON. After four injections of DOCA (10 mg/rat per day), rats developed a salt appetite and increased NADPH-d active and nNOS-IR neurons in both nuclei. A single DOCA injection did not change salt consumption or nNOS-IR cells, but increased the number of NADPH-d positive neurons in the PVN only. Therefore, while acute MC treatment stimulated the activity of pre-existing enzyme, chronic steroid treatment recruited additional neurons showing nNOS immunoreactivity/NADPH-d activity. These data suggest a role for NO produced in the PVN and SON in DOCA stimulatory effects on AVP mRNA and salt appetite. © 2002 Published by Elsevier Science Ireland Ltd.

Registro:

Documento: Artículo
Título:Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
Autor:Saravia, F.E.; Pietranera, L.; Lima, A.; Roig, P.; Revsin, Y.; De Nicola, A.F.
Filiación:Laboratory of Neuroendocrine Biochemistry, Instituto de Biología y Medicina Experimental, 1428 Buenos Aires, Argentina
Department of Human Biochemistry, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
Palabras clave:Mineralocorticoids; Nicotinamide dinucleotide diphoshate-diaphorase; Nitric oxide synthase; Salt appetite; argipressin; cytochrome b5 reductase; deoxycorticosterone acetate; messenger RNA; nicotinamide adenine dinucleotide phosphate; nitric oxide; nitric oxide synthase; sodium chloride; animal cell; animal experiment; animal tissue; anterior hypothalamus; appetite; brain nerve cell; cell count; controlled study; drug effect; enzyme activation; enzyme activity; food intake; hypothalamus nucleus; immunocompetent cell; immunoreactivity; male; nonhuman; priority journal; rat; review; supraoptic nucleus
Año:2002
Volumen:329
Número:3
Página de inicio:344
Página de fin:348
DOI: http://dx.doi.org/10.1016/S0304-3940(02)00666-3
Título revista:Neuroscience Letters
Título revista abreviado:Neurosci. Lett.
ISSN:03043940
CODEN:NELED
CAS:argipressin, 113-79-1; cytochrome b5 reductase, 9032-25-1, 9047-21-6; deoxycorticosterone acetate, 56-47-3; nicotinamide adenine dinucleotide phosphate, 53-59-8; nitric oxide, 10102-43-9; nitric oxide synthase, 125978-95-2; sodium chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v329_n3_p344_Saravia

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Citas:

---------- APA ----------
Saravia, F.E., Pietranera, L., Lima, A., Roig, P., Revsin, Y. & De Nicola, A.F. (2002) . Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats. Neuroscience Letters, 329(3), 344-348.
http://dx.doi.org/10.1016/S0304-3940(02)00666-3
---------- CHICAGO ----------
Saravia, F.E., Pietranera, L., Lima, A., Roig, P., Revsin, Y., De Nicola, A.F. "Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats" . Neuroscience Letters 329, no. 3 (2002) : 344-348.
http://dx.doi.org/10.1016/S0304-3940(02)00666-3
---------- MLA ----------
Saravia, F.E., Pietranera, L., Lima, A., Roig, P., Revsin, Y., De Nicola, A.F. "Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats" . Neuroscience Letters, vol. 329, no. 3, 2002, pp. 344-348.
http://dx.doi.org/10.1016/S0304-3940(02)00666-3
---------- VANCOUVER ----------
Saravia, F.E., Pietranera, L., Lima, A., Roig, P., Revsin, Y., De Nicola, A.F. Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats. Neurosci. Lett. 2002;329(3):344-348.
http://dx.doi.org/10.1016/S0304-3940(02)00666-3