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Abstract:

The quantitation of four polyamines in hypothalamus and pituitary is studied in male and female developing rats using an improved high-performance liquid chromatography method. In the hypothalamus, putrescine (PUT) reaches the highest concentration (nmol/mg protein) on day 6. It shows the lowest value in comparison with any other polyamine. Spermidine (SPD) is high during the first postnatal days. Spermine (SPM) fluctuates, and agmatine (AGM) is highest during the first week. SPD, SPM and AGM are lower in females. In the pituitary, PUT, SPD and AGM are high during the first week. SPM remains constant and it is higher in males. AGM is higher in males only on day 1. PUT shows the lowest concentration of all. Concentrations of PUT, SPD and SPM are higher in the pituitary; AGM is higher in the hypothalamus. α-Difluoromethylornithine (a specific and irreversible inhibitor of ornithine decarboxylase) decreases PUT and SPD, increased SPM and AGM remain unchanged in the hypothalamus and pituitary. Thus, each polyamine has its own pattern in hypothalamus and in pituitary during development in males and females; these changes could be related to the hypothalamic control of pituitary secretion of hormones related to reproduction in mammals. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
Autor:Thyssen, S.M.; Libertun, C.
Filiación:Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina
Department of Physiology, University of Buenos Aires Medical School, Buenos Aires, Argentina
Laboratorio de Neuroendocrinolog, Instituto de Biologa y Medicina Experimental-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Development; Hypothalamus; Pituitary; Polyamines; Rat; Sexes; α-Difluoromethylornithine; agmatine; eflornithine; ornithine decarboxylase inhibitor; polyamine derivative; putrescine; spermidine; animal experiment; article; controlled study; diagnostic value; dose response; female; high performance liquid chromatography; hypophysis; hypophysis function; hypothalamus; male; mammal; nonhuman; perinatal period; priority journal; quantitative analysis; rat; reproduction
Año:2002
Volumen:323
Número:1
Página de inicio:65
Página de fin:69
DOI: http://dx.doi.org/10.1016/S0304-3940(02)00097-6
Título revista:Neuroscience Letters
Título revista abreviado:Neurosci. Lett.
ISSN:03043940
CODEN:NELED
CAS:agmatine, 306-60-5; eflornithine, 67037-37-0, 70052-12-9; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v323_n1_p65_Thyssen

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Citas:

---------- APA ----------
Thyssen, S.M. & Libertun, C. (2002) . Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats. Neuroscience Letters, 323(1), 65-69.
http://dx.doi.org/10.1016/S0304-3940(02)00097-6
---------- CHICAGO ----------
Thyssen, S.M., Libertun, C. "Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats" . Neuroscience Letters 323, no. 1 (2002) : 65-69.
http://dx.doi.org/10.1016/S0304-3940(02)00097-6
---------- MLA ----------
Thyssen, S.M., Libertun, C. "Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats" . Neuroscience Letters, vol. 323, no. 1, 2002, pp. 65-69.
http://dx.doi.org/10.1016/S0304-3940(02)00097-6
---------- VANCOUVER ----------
Thyssen, S.M., Libertun, C. Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats. Neurosci. Lett. 2002;323(1):65-69.
http://dx.doi.org/10.1016/S0304-3940(02)00097-6