Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The protein substrates affected by staurosporine (SP), the most potent inhibitor of protein kinases yet described, are unknown. In order to approach this problem we incubated cerebral cortex tissue with 0, 20, 50 and 100 nM of SP using [32P]orthophosphate as radioactive precursor. The analysis of the phosphoproteins were made with a modified high resolution two dimensional gel electrophoresis, followed by autoradiography. We detected several proteins affected by SP. Specially noticeable was an approximately 55 kDa protein which strikingly diminished the intensity of phosphorylation. However, the reverse phenomenon was also observed. To the best of our knowledge this is the first examination of protein substrates affected by SP in intact tissue. © 1994.

Registro:

Documento: Artículo
Título:Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex
Autor:Sebastian Yakisich, J.; Radrizzani, M.; Vargas, V.I.
Filiación:Instituto de Biologia Celular, Facultad de Medicina U.B.A., Avda Patricias Argentinas 435, C.P. 1405 Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Avda Patricias Argentinas 435, C.P. 1405 Buenos Aires, Argentina
Palabras clave:Cerebral cortex; Development; Phosphoprotein; Phosphorylation; Protein kinase; Staurosporine; staurosporine; adolescent; animal tissue; article; brain cortex; brain development; controlled study; gel electrophoresis; newborn; nonhuman; priority journal; protein phosphorylation; rat; Alkaloids; Animal; Autoradiography; Cerebral Cortex; Electrophoresis, Polyacrylamide Gel; Indicators and Reagents; Nerve Tissue Proteins; Phosphorylation; Protein Kinase C; Rats; Rats, Wistar; Rosaniline Dyes; Staurosporine
Año:1994
Volumen:180
Número:1
Página de inicio:17
Página de fin:20
DOI: http://dx.doi.org/10.1016/0304-3940(94)90903-2
Título revista:Neuroscience Letters
Título revista abreviado:Neurosci. Lett.
ISSN:03043940
CODEN:NELED
CAS:Alkaloids; Coomassie blue, 78642-64-5; Indicators and Reagents; Nerve Tissue Proteins; Protein Kinase C, EC 2.7.1.37; Rosaniline Dyes; Staurosporine, 62996-74-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v180_n1_p17_SebastianYakisich

Referencias:

  • Alperin, Idoyaga-Vargas, Carminatti, Rate of protein glycosylation in rat cerebral cortex (1986) J. Neurochem., 47, pp. 355-362
  • Armstrong, Montminy, Transsynaptic control of gene expression (1993) Annu. Rev. Neurosci., 16, pp. 17-29
  • Baltuch, Shenouda, Langleben, Villemure, High dose tamoxifen in the treatment of recurrent high grade glioma (1993) A report of clinical stabilization and tumor regression, 20, pp. 168-176. , Can. J. Neurol. Sci
  • Bedoy, Mobley, Astrocyte morphology altered by 1-(5-isoquinolinylsulfonyl) 2-methyl piperazine (H-7) and other protein kinase inhibitors (1989) Brain Res., 490, pp. 243-254
  • Berry, Rogers, Eayrs, Pattern of cell migration during cortical histogenesis (1964) Nature, 203, pp. 591-593
  • Cohen, Signal integration at the level of protein kinases, protein phosphatases and their substrates (1992) Trends Biol. Sci., 17, pp. 408-413
  • Couldwell, Uhm, Antel, Young, Enhanced Protein Kinase C activity correlates with the growth rate of malignant gliomas in vitro (1991) Neurosurgery, 29, pp. 880-887
  • Couldwell, Antel, Yong, Protein Kinase C activity correlates with the growth rate of malignant gliomas. Part II. Effects of glioma mitogens and modulators of Protein Kinase C (1992) Neurosurgery, 31, pp. 717-724
  • Couldwell, Weiss, Degiorgio, Weiner, Hinton, Ehresman, Conti, Detribolet, Clinical and radiographic response in a minority of patients with recurrent malignant gliomas treated with high-dose tamoxifen (1993) Neurosurgery, 32, pp. 485-490
  • Finkbeiner, Glial Calcium (1993) Glia, 9, pp. 83-104
  • Gamou, Shimizu, Calphostin-C stimulates epidermal growth factor receptor phosphorylation and internalization via light-dependent mechanism (1994) J. Cell Physiol., 158, pp. 151-159
  • Greengard, Protein phosphorylation and the regulation of neuronal function (1989) Basic Neurochemistry, Molecular, Cellular and Medical Aspects, , Siegel et al., 4th edn., Raven, New York
  • Hashimoto, Hagino, Staurosporine-induced neurite outgrowth in PC12h cells (1980) Exp. Cell Res., 184, pp. 351-359
  • Hennipman, van Oirschot, Smits, Rijksen, Staal, Tyrosine kinase activity in breast cancer, benign breast disease, and normal breast tissue (1989) Cancer Res., 49, pp. 516-521
  • Jessell, Kandel, Synaptic transmission: a bidirectional and self-modifiable form of cell-cell communication (1993) Cell, 72, pp. 1-30
  • Johnson, Villancourt, Sequential protein kinase reactions controling cell growth and differentiation (1994) Curr. Opinion Cell Biol., 6, pp. 230-238
  • Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Lowry, Rosebrough, Farr, Randall, Protein measurement with the folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Macfarlane, Two dimensional benzyldimethyl-n-hexadecyl-ammonium chloride → sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: a high capacity high resolution technique for the purification of proteins from complex mixtures (1989) Anal. Biochem., 176, pp. 457-463. , Erratum 180, pp 194
  • Patterson, Nawa, Neuronal differeniation factor/cytokines and synaptic plasticity (1993) Cell, 72, pp. 123-137
  • Raff, Barres, Burne, Coles, Ishizaki, Jacobson, Programmed cell death and the control of cell survival: lessons from the nervous system (1993) Science, 262, pp. 695-700
  • Rasouly, Rahamim, Lester, Matsuda, Lazarovici, Staurosporine-induced neurite outgrowth in PC12h cells is independent of Protein Kinase C inhibition (1992) Mol. Pharmacol., 42, pp. 35-43
  • Tamaoki, Use and specificity of Staurosporine, UCN-01, and Calphostin C as protein kinase inhibitors (1991) Methods Enzymol., 201, pp. 340-347
  • van Erp, Rijksen, van Veelen, van der Heijden, Maarschalkerweerd, Staal, Protein Tyrosine Kinase in human brain and gliomas (1992) J. Neurochem., 58, pp. 554-561
  • Vertosick, Selker, Pollack, Arena, The treatment of intracraneal malignant gliomas using orally administered Tamoxifen therapy: preliminary results in a series of ‘failed’ patients (1992) Neurosurgery, 30, pp. 897-903

Citas:

---------- APA ----------
Sebastian Yakisich, J., Radrizzani, M. & Vargas, V.I. (1994) . Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex. Neuroscience Letters, 180(1), 17-20.
http://dx.doi.org/10.1016/0304-3940(94)90903-2
---------- CHICAGO ----------
Sebastian Yakisich, J., Radrizzani, M., Vargas, V.I. "Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex" . Neuroscience Letters 180, no. 1 (1994) : 17-20.
http://dx.doi.org/10.1016/0304-3940(94)90903-2
---------- MLA ----------
Sebastian Yakisich, J., Radrizzani, M., Vargas, V.I. "Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex" . Neuroscience Letters, vol. 180, no. 1, 1994, pp. 17-20.
http://dx.doi.org/10.1016/0304-3940(94)90903-2
---------- VANCOUVER ----------
Sebastian Yakisich, J., Radrizzani, M., Vargas, V.I. Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex. Neurosci. Lett. 1994;180(1):17-20.
http://dx.doi.org/10.1016/0304-3940(94)90903-2