Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Binding of heparin to primary cultured cells of two murine mammary adenocarcinomas with low (M3) and high (MM3) lung, metastatic capacity was determined. Heparin binding was rapid, specific and saturable. MM3 cells grown for 24 h in fetal calf serum (FCS)-free medium exhibited a higher number of binding sites for 3H-heparin [(11 ± 1) × 105 sites per cell] than M3 cells [(6.9 ± 0.6) × 105 sites per cell]. However, when M3 cells were grown in the presence of 2% FCS, they showed less heparin binding sites [(3.5 ± 0.4) × 105 sites per cell]. In contrast, dissociation constants were very similar for MM3 and M3 cells grown with or without FCS (Kd = 2-4 × 10-9M). Furthermore, heparin inhibited MM3 and M3 cell growth both in the absence or presence of FCS. Competition studies showed that chemically modified heparins lacking antiproliferative effect (O-desulfated; O/N-desulfated N-acetylated and N-desulfated heparins) were not able to inhibit 3H-heparin binding. N-desulfated N-acetylated heparin, which had partial antiproliferative effect, partially inhibited 3H-heparin binding, while heparin with a high antiproliferative activity inhibited more than 90% 3H-heparin binding. The antiproliferative effect of heparin and chemically modified heparins seems to be related to their binding ability to the cell membrane. © 1995.

Registro:

Documento: Artículo
Título:Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition
Autor:Bertolesix, G.E.; de Cidre, L.L.; de Lustig, E.S.; Eiján, A.M.
Filiación:Area Investigación, Institute de Oncologia A.H. Roffo, Universidad de Buenos Aires, Av. San Martín 5481, Buenos Aires, 1417, Argentina
Laboratorio de Histologia Animal, Departamento de Biologia, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, Buenos Aires 1428, Argentina, Argentina
Palabras clave:Growth inhibition; Heparin; Metastasis; Receptor; Tumor; heparin; membrane receptor; tritium; acetylation; animal cell; animal experiment; animal model; animal tissue; article; breast adenocarcinoma; cell proliferation; controlled study; desulfurization; dissociation constant; female; fetal calf serum; growth inhibition; lung metastasis; mouse; nonhuman; priority journal; Adenocarcinoma; Analysis of Variance; Animal; Antineoplastic Agents; Binding, Competitive; Cell Division; Female; Heparin; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Neoplasm Metastasis; Receptors, Cell Surface; Support, Non-U.S. Gov't; Tumor Cells, Cultured; Animalia; Murinae; Tritium
Año:1995
Volumen:90
Número:2
Página de inicio:123
Página de fin:131
DOI: http://dx.doi.org/10.1016/0304-3835(95)03693-Q
Título revista:Cancer Letters
Título revista abreviado:Cancer Lett.
ISSN:03043835
CODEN:CALED
CAS:Antineoplastic Agents; heparin receptor; Heparin, 9005-49-6; Receptors, Cell Surface
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v90_n2_p123_Bertolesix

Referencias:

  • Bal de Kier Joffé, Puricelli, Vidal, Sacerdote de Lustig, Characterization of two murine mammary adenocarcinoma tumors with different metastatic ability (1983) J. Exp. Clin. Cancer Res., 2, pp. 151-160
  • Bal de Kier Joffé, Puricelli, Sacerdote de Lustig, Modified adhesion behaivour after in vitro passage of two-related murine mammary adenocarcinomas with different metastasizing ability (1986) Invasion Metastasis, 6, pp. 302-312
  • Bertolesi, Lauria de Cidre, Eiján, Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins (1994) Tumor Biol., 15, pp. 275-283
  • Biswas, Heparin and heparan sulfate binding sites on B16 melanoma cells (1988) J. Cell. Physiol., 136, pp. 147-153
  • Castellot, Beeker, Rosenberg, Karnovsky, Structural determinants of the capacity of heparin to inhibit the proliferation of vascular smooth muscle cells (1984) J. Cell. Physiol., 120, pp. 315-320
  • Castellot, Wong, Herman, Hoover, Albertini, Wrigth, Caleb, Karnovsky, Binding and internalization of heparin by vascular smooth muscle cells (1985) J. Cell. Physiol., 124, pp. 13-20
  • Castellot, Pukac, Caleb, Wrigth, Karnovsky, Heparin selectively inhibits a protein kinase C-dependent mechanism of cell cycle progression in calf aortic smooth muscle cells (1989) J. Cell Biol., 109, pp. 3147-3155
  • Clowes, Karnovsy, Suppression by heparin of smooth muscle cell proliferation in injured arteries (1977) Nature, 265, pp. 625-626
  • Evans, Marshall, Christey, Carrel, Heparin binding site, conformational change and activition of antithrombin (1992) Biochemistry, 31, pp. 12629-12642
  • Glimelius, Busch, Hook, Binding of heparin on the surface of cultured human endothelial cells (1978) Tromb. Res., 12, pp. 773-782
  • Halper, Carter, Modulation of growth of human carcinoma SW-13 cells by heparin and growth factors (1989) J. Cell. Physiol., 141, pp. 16-23
  • Iozzo, Proteoglycans and neoplasia (1988) Cancer Metastasis Rev., 7, pp. 39-50
  • Kjellen, Oldberg, Rubin, Hook, Binding of heparin and heparan sulfate to rat liver cells (1977) Biochem. Biophys. Res. Commun., 74, pp. 126-133
  • Lindahl, Bñckström, Thunberg, The antithrombin-binding sequence in heparin. Identification of an essential 6-O-sulfate group (1983) J. Biol. Chem., 16, pp. 9826-9830
  • Labarca, Paigen, A simple, rapid and sensitive DNA assay procedure (1980) Anal. Biochem., 102, pp. 344-352
  • Ladeda, Puricelli, Urtreger, Alonso, Kornblihtt, Ball de Kier Joffé, Acción del Suero y de Inhibidores Proteásicos Sobre la Expresión in vitro de Fibronectina (1994) VI Congreso de La Sociedad Argentina de Ciencias Morfológicas, p. 91a
  • Leung, Katsuyasu, Grant, Heparin binds to human monocytes and modulates their procoagulant activities and secretory phenotypes. Effect of histidine-rich glycoprotein (1989) Blood, 73, pp. 177-184
  • Muro, Puricelli, Kornblihtt, Bal de Kier Joffe, Inverse correlation between fibronectin mRNA levels and the metastatic potential of two murine mammary adenocarcinomas (1991) Invation Metastasis, 11, pp. 281-287
  • Ottlinger, Pukac, Karnovsky, Heparin inhibits mitogenic actived protein kinase activation in intact rat vascular smooth muscle cells (1993) J. Biol. Chem., 268, pp. 19173-19176
  • Oyama, Eagle, Measurament of cell growth in tissue culture with phenol reagent (folinciocalteau) (1956) Proc. Soc. Exp. Biol. Med., 91, pp. 305-307. , Ed. 2
  • Pereyra-Alfonso, Haedo, Bal de Kier Joffé, Correlation betwen urokinase-type plasminogen activator production and the metastasizing ability of two murine mammary adenocarcinomas (1988) Int. J. Cancer, 42, pp. 59-63
  • Pukac, Castellot, Wrigth, Caleb, Karnovsky, Heparin inhibits c-fos and c-myc mRNA expression in vascular smooth muscle cells (1990) Cell Regul, 1, pp. 435-443
  • Pukac, Ottlinger, Karnovsky, Heparin supresses specific second messenger pathways for protooncogene expression in rat vascular smooth muscle cells (1992) J. Biol. Chem., 267, pp. 3707-3711
  • Redini, Moczar, Antoine, Poupon, Binding and internalization of exogenous glycosaminoglicans in weakly and highly metastatic rhabdomyosarcoma cells (1989) Biochem. Biophys. Acta, 991, pp. 359-366
  • Reilly, Fritze, Rosenberg, Heparin-Like molecules regulate the number of epidermal growth factors on vascular smooth muscle cell (1988) Journal of Cellular Physiology, 136, pp. 26-32
  • Reilly, Kindy, Brown, Rosenberg, Sonenshein, Heparin prevents vascular smooth muscle cell progression through the G1 phase of the cell cycle (1989) J. Biol. Chem., 264, pp. 6990-6995
  • San Antonio, Slover, Lawler, Karnovsky, Lander, Specificity in the interactions of extracellular matrix proteins with subpopulations of the glycosaminoglycan heparin (1993) Biochem., 32, pp. 4746-4755
  • Wrigth, Jr., Pukac, Castellot, Karnovsky, Levine, Kim-Park, Campisi, Heparin suppresses the induction of c-fos and c-myc mRNA in murine fibroblasts by selective inhibition of a protein kinase C-dependent pathway (1989) Proceedings of the National Academy of Sciences, 86, pp. 3199-3203. , Ed. 2

Citas:

---------- APA ----------
Bertolesix, G.E., de Cidre, L.L., de Lustig, E.S. & Eiján, A.M. (1995) . Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition. Cancer Letters, 90(2), 123-131.
http://dx.doi.org/10.1016/0304-3835(95)03693-Q
---------- CHICAGO ----------
Bertolesix, G.E., de Cidre, L.L., de Lustig, E.S., Eiján, A.M. "Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition" . Cancer Letters 90, no. 2 (1995) : 123-131.
http://dx.doi.org/10.1016/0304-3835(95)03693-Q
---------- MLA ----------
Bertolesix, G.E., de Cidre, L.L., de Lustig, E.S., Eiján, A.M. "Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition" . Cancer Letters, vol. 90, no. 2, 1995, pp. 123-131.
http://dx.doi.org/10.1016/0304-3835(95)03693-Q
---------- VANCOUVER ----------
Bertolesix, G.E., de Cidre, L.L., de Lustig, E.S., Eiján, A.M. Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition. Cancer Lett. 1995;90(2):123-131.
http://dx.doi.org/10.1016/0304-3835(95)03693-Q