Ibañez, I.L.; Policastro, L.L.; Tropper, I.; Bracalente, C.; Palmieri, M.A.; Rojas, P.A.; Molinari, B.L.; Durán, H. "H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1" (2011) Cancer Letters. 305(1):58-68
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The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory protein p27KIP1. Moreover, we found a differential intracellular distribution of p27KIP1, which remained in the nucleus after catalase treatment. In vivo experiments showed an increase in nuclear levels of p27KIP1 associated with the inhibition of tumor growth by H2O2 scavenging, confirming in vitro results. To conclude, H2O2 scavenging may induce cell cycle arrest through the modulation of cyclin D1 and p27KIP1 levels and nuclear localization of p27KIP1. To our knowledge, this is the first report that demonstrates that the modulation of ROS alters the intracellular localization of a key regulatory protein of G1/S transition. © 2011 Elsevier Ireland Ltd.


Documento: Artículo
Título:H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
Autor:Ibañez, I.L.; Policastro, L.L.; Tropper, I.; Bracalente, C.; Palmieri, M.A.; Rojas, P.A.; Molinari, B.L.; Durán, H.
Filiación:Comisión Nacional de Energía Atómica, Av. Gral. Paz 1499, (B1650KNA) San Martín, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Cancer cells; Cell cycle; Cyclin D1; Hydrogen peroxide; P27kip1; Proliferation; catalase; cyclin D1; cyclin dependent kinase inhibitor 1B; hydrogen peroxide; catalase; cyclin D1; cyclin dependent kinase inhibitor 1B; hydrogen peroxide; reactive oxygen metabolite; scavenger; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; article; cancer inhibition; carcinoma cell; cell cycle arrest; cell cycle G1 phase; cell cycle regulation; cell cycle S phase; cell level; cell nucleus; cell proliferation; cellular distribution; controlled study; cytoplasm; female; in vitro study; in vivo study; intracellular space; mouse; nonhuman; priority journal; protein analysis; protein localization; protein modification; squamous cell carcinoma; active transport; animal; drug effect; fluorescent antibody technique; metabolism; nude mouse; sensitive to carcinogenesis mouse; tumor cell line; Western blotting; Active Transport, Cell Nucleus; Animals; Blotting, Western; Catalase; Cell Line, Tumor; Cell Nucleus; Cyclin D1; Cyclin-Dependent Kinase Inhibitor p27; Female; Fluorescent Antibody Technique; Free Radical Scavengers; G1 Phase; Hydrogen Peroxide; Mice; Mice, Inbred SENCAR; Mice, Nude; Reactive Oxygen Species; S Phase
Página de inicio:58
Página de fin:68
Título revista:Cancer Letters
Título revista abreviado:Cancer Lett.
CAS:catalase, 9001-05-2; hydrogen peroxide, 7722-84-1; Catalase,; Cyclin D1, 136601-57-5; Cyclin-Dependent Kinase Inhibitor p27, 147604-94-2; Free Radical Scavengers; Hydrogen Peroxide, 7722-84-1; Reactive Oxygen Species


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---------- APA ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., Molinari, B.L.,..., Durán, H. (2011) . H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1. Cancer Letters, 305(1), 58-68.
---------- CHICAGO ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., et al. "H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1" . Cancer Letters 305, no. 1 (2011) : 58-68.
---------- MLA ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., et al. "H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1" . Cancer Letters, vol. 305, no. 1, 2011, pp. 58-68.
---------- VANCOUVER ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., et al. H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1. Cancer Lett. 2011;305(1):58-68.