Artículo

Ibañez, I.L.; Policastro, L.L.; Tropper, I.; Bracalente, C.; Palmieri, M.A.; Rojas, P.A.; Molinari, B.L.; Durán, H. "H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1" (2011) Cancer Letters. 305(1):58-68
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The aim of the present study was to evaluate cell cycle regulation by scavenging H2O2 in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory protein p27KIP1. Moreover, we found a differential intracellular distribution of p27KIP1, which remained in the nucleus after catalase treatment. In vivo experiments showed an increase in nuclear levels of p27KIP1 associated with the inhibition of tumor growth by H2O2 scavenging, confirming in vitro results. To conclude, H2O2 scavenging may induce cell cycle arrest through the modulation of cyclin D1 and p27KIP1 levels and nuclear localization of p27KIP1. To our knowledge, this is the first report that demonstrates that the modulation of ROS alters the intracellular localization of a key regulatory protein of G1/S transition. © 2011 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
Autor:Ibañez, I.L.; Policastro, L.L.; Tropper, I.; Bracalente, C.; Palmieri, M.A.; Rojas, P.A.; Molinari, B.L.; Durán, H.
Filiación:Comisión Nacional de Energía Atómica, Av. Gral. Paz 1499, (B1650KNA) San Martín, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Cancer cells; Cell cycle; Cyclin D1; Hydrogen peroxide; P27kip1; Proliferation; catalase; cyclin D1; cyclin dependent kinase inhibitor 1B; hydrogen peroxide; catalase; cyclin D1; cyclin dependent kinase inhibitor 1B; hydrogen peroxide; reactive oxygen metabolite; scavenger; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; article; cancer inhibition; carcinoma cell; cell cycle arrest; cell cycle G1 phase; cell cycle regulation; cell cycle S phase; cell level; cell nucleus; cell proliferation; cellular distribution; controlled study; cytoplasm; female; in vitro study; in vivo study; intracellular space; mouse; nonhuman; priority journal; protein analysis; protein localization; protein modification; squamous cell carcinoma; active transport; animal; drug effect; fluorescent antibody technique; metabolism; nude mouse; sensitive to carcinogenesis mouse; tumor cell line; Western blotting; Active Transport, Cell Nucleus; Animals; Blotting, Western; Catalase; Cell Line, Tumor; Cell Nucleus; Cyclin D1; Cyclin-Dependent Kinase Inhibitor p27; Female; Fluorescent Antibody Technique; Free Radical Scavengers; G1 Phase; Hydrogen Peroxide; Mice; Mice, Inbred SENCAR; Mice, Nude; Reactive Oxygen Species; S Phase
Año:2011
Volumen:305
Número:1
Página de inicio:58
Página de fin:68
DOI: http://dx.doi.org/10.1016/j.canlet.2011.02.026
Título revista:Cancer Letters
Título revista abreviado:Cancer Lett.
ISSN:03043835
CODEN:CALED
CAS:catalase, 9001-05-2; hydrogen peroxide, 7722-84-1; Catalase, 1.11.1.6; Cyclin D1, 136601-57-5; Cyclin-Dependent Kinase Inhibitor p27, 147604-94-2; Free Radical Scavengers; Hydrogen Peroxide, 7722-84-1; Reactive Oxygen Species
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043835_v305_n1_p58_Ibanez

Referencias:

  • Davies, K.J., Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems (2000) IUBMB Life, 50, pp. 279-289
  • Arnold, R.S., Shi, J., Murad, E., Whalen, A.M., Sun, C.Q., Polavarapu, R., Parthasarathy, S., Lambeth, J.D., Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1 (2001) Proc. Natl. Acad. Sci. USA, 98, pp. 5550-5555
  • Policastro, L., Molinari, B., Larcher, F., Blanco, P., Podhajcer, O.L., Costa, C.S., Rojas, P., Durán, H., Imbalance of antioxidant enzymes in tumor cells and inhibition of proliferation and malignant features by scavenging hydrogen peroxide (2004) Mol. Carcinog., 39, pp. 103-113
  • Laurent, A., Nicco, C., Chéreau, C., Goulvestre, C., Alexandre, J., Alves, A., Lévy, E., Batteux, F., Controlling tumor growth by modulating endogenous production of reactive oxygen species (2005) Cancer Res., 65, pp. 948-956
  • Antunes, F., Cadenas, E., Cellular titration of apoptosis with steady state concentrations of H2O2: submicromolar levels of H2O2 induce apoptosis through Fenton chemistry independent of the cellular thiol state (2001) Free Radical Biol. Med., 30, pp. 1008-1018
  • Carreras, M.C., Converso, D.P., Lorenti, A.S., Barbich, M., Levisman, D.M., Jaitovich, A., Antico Arciuch, V.G., Poderoso, J.J., Mitochondrial nitric oxide synthase drives redox signals for proliferation and quiescence in rat liver development (2004) Hepatology, 40, pp. 157-166
  • Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., Telser, J., Free radicals and antioxidants in normal physiological functions and human disease (2007) Int. J. Biochem. Cell Biol., 39, pp. 44-84
  • Halliwel, B., Oxidative stress and cancer: have we moved forward? (2007) Biochem. J., 401, pp. 1-11
  • Irani, K., Xia, Y., Zweier, J.L., Sollot, S.J., Der, C.J., Fearon, E.R., Sundaresan, M., Goldschmidt-Clermont, P.J., Mitogenic signaling mediated by oxidants in ras-transformed fibroblasts (1997) Science, 275, pp. 1649-1652
  • Ushio-Fukai, M., Redox signaling in angiogenesis: role of NADPH oxidase (2006) Cardiovasc. Res., 71, pp. 226-235
  • Nelson, K.K., Ranganathan, A.C., Mansouri, J., Rodriguez, A.M., Providence, K.M., Rutter, J.L., Pumiglia, K., Melendez, J.A., Elevated sod2 activity augments matrix metalloproteinase expression: evidence for the involvement of endogenous hydrogen peroxide in regulating metastasis (2003) Clin. Cancer Res., 9, pp. 424-432
  • McCord, J.M., Superoxide radical: controversies, contradictions, and paradoxes (1995) Proc. Soc. Exp. Biol. Med., 209, pp. 112-117
  • Szatrowski, T.P., Nathan, C.F., Production of large amounts of hydrogen peroxide by human tumor cells (1991) Cancer Res., 51, pp. 794-798
  • Policastro, L.L., Ibañez, I.L., Durán, H.A., Soria, G., Gottifredi, V., Podhajcer, O.L., Suppression of cancer growth by nonviral gene therapy based on a novel reactive oxygen species-responsive promoter (2009) Mol. Ther., 17, pp. 1355-1364
  • Toyokuni, S., Okamoto, K., Yodoi, J., Hiai, H., Persistent oxidative stress in cancer (1995) FEBS Lett., 358, pp. 1-3
  • Kondo, S., Toyokuni, S., Iwasa, Y., Tanaka, T., Onodera, H., Hiai, H., Imamura, M., Persistent oxidative stress in human colorectal carcinoma, but not in adenoma (1999) Free Radical Biol. Med., 27, pp. 401-410
  • Hempel, N., Ye, H., Abessi, B., Mian, B., Melendez, J.A., Altered redox status accompanies progression to metastatic human bladder cancer (2009) Free Radical Biol. Med., 46, pp. 42-50
  • Guyton, K.Z., Kensler, T.W., Oxidative mechanisms in carcinogenesis (1993) Br. Med. Bull., 49, pp. 523-544
  • Cerutti, P.A., Prooxidant states and tumor promotion (1985) Science, 227, pp. 375-381
  • Hyoudou, K., Nishikawa, M., Ikemura, M., Kobayashi, Y., Mendelsohn, A., Miyazaki, N., Tabata, Y., Hashida, M., Prevention of pulmonary metastasis from subcutaneous tumors by binary system-based sustained delivery of catalase (2009) J. Controlled Release, 137, pp. 110-115
  • Onumah, O.E., Jules, G.E., Zhao, Y., Zhou, L., Yang, H., Guo, Z., Overexpression of catalase delays G0/G1- to S-phase transition during cell cycle progression in mouse aortic endothelial cells (2009) Free Radical Biol. Med., 46, pp. 1658-1667
  • Stone, J.R., Yang, S., Hydrogen peroxide: a signaling messenger (2006) Antioxid. Redox Signal., 8, pp. 243-270
  • Cakir, Y., Ballinger, S.W., Reactive species-mediated regulation of cell signaling and the cell cycle: the role of MAPK (2005) Antioxid. Redox Signal., 7, pp. 726-740
  • Benhar, M., Engelberg, D., Levitzki, A., ROS, stress-activated kinases and stress signaling in cancer (2002) EMBO Rep., 3, pp. 420-425
  • Elledge, S.J., Cell cycle checkpoints: preventing an identity crisis (1996) Science, 274, pp. 1664-1672
  • Sherr, C.J., Cancer cell cycles (1996) Science, 274, pp. 1672-1677
  • Borriello, A., Cucciola, V., Oliva, A., Zappia, V., Della Ragione, F., P27KIP1 metabolism: a fascinating labyrinth (2007) Cell Cycle, 6, pp. 1053-1061
  • Menon, S.G., Sarsour, E.H., Spitz, D.R., Higashikubo, R., Sturm, M., Zhang, H., Goswami, P.C., Redox regulation of the G1 to S phase transition in the mouse embryo fibroblast cell cycle (2003) Cancer Res., 63, pp. 2109-2117
  • Sarsour, E.H., Kumar, M.G., Chaudhuri, L., Kalenand, A.L., Goswami, P.C., Redox control of the cell cycle in health and disease (2009) Antioxid. Redox Signal., 11, pp. 2985-3011
  • Burch, P.M., Heintz, N.H., Redox regulation of cell-cycle re-entry: cyclin D1 as a primary target for the mitogenic effects of reactive oxygen and nitrogen species (2005) Antioxid. Redox Signal., 7, pp. 741-751
  • Felty, Q., Singh, K.P., Roy, D., Estrogen-induced G1/S transition of G0-arrested estrogen-dependent breast cancer cells is regulated by mitochondrial oxidant signaling (2005) Oncogene, 24, pp. 4883-4893
  • Conti, C.J., Fries, J.W., Viaje, A., Miller, D.R., Morris, R., Slaga, T.J., In vivo behavior of murine epidermal cell lines derived from initiated and noninitiated skin (1988) Cancer Res., 48, pp. 435-439
  • Liu, Y., Peterson, D.A., Kimura, H., Schubert, D., Mechanism of cellular 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) reduction (1997) J. Neurochem., 69, pp. 581-593
  • Deng, X., Gao, F., May, W.S., Bcl2 retards G1/S cell cycle transition by regulating intracellular ROS (2003) Blood, 102, pp. 3179-3185
  • Martín, V., Herrera, F., García-Santos, G., Antolín, I., Rodriguez-Blanco, J., Rodriguez, C., Signaling pathways involved in antioxidant control of glioma cell proliferation (2007) Free Radical Biol. Med., 42, pp. 1715-1722
  • Preston, T.J., Muller, W.J., Singh, G., Scavenging of extracellular H2O2 by catalase inhibits the proliferation of HER-2/Neu-transformed rat-1 fibroblasts through the induction of a stress response (2001) J. Biol. Chem., 276, pp. 9558-9564
  • Weber, J.D., Raben, D.M., Phillips, P.J., Baldassare, J.J., Sustained activation of extracellular-signal-regulated kinase 1 (ERK1) is required for the continued expression of cyclin D1 in G1 phase (1997) Biochem. J., 326, pp. 61-68
  • Menon, S.G., Sarsour, E.H., Kalen, A.L., Venkataraman, S., Hitchler, M.J., Domann, F.E., Oberley, L.W., Goswami, P.C., Superoxide signaling mediates N-acetyl-l-cysteine-induced G1 arrest: regulatory role of cyclin D1 and manganese superoxide dismutase (2007) Cancer Res., 67, pp. 6392-6399
  • Barnouin, K., Dubuisson, M.L., Child, E.S., Fernandez de Mattos, S., Glassford, J., Medema, R.H., Mann, D.J., Lam, E.W., H2O2 induces a transient multi-phase cell cycle arrest in mouse fibroblasts through modulating cyclin D and p21Cip1 expression (2002) J. Biol. Chem., 277, pp. 13761-13770
  • Tashiro, E., Tsuchiya, A., Imoto, M., Functions of cyclin D1 as an oncogene and regulation of cyclin D1 expression (2007) Cancer Sci., 98, pp. 629-635
  • Robles, A.I., Conti, C.J., Early overexpression of cyclin D1 protein in mouse skin carcinogenesis (1995) Carcinogenesis, 16, pp. 781-786
  • Rodríguez-Puebla, M.L., Robles, A.I., Conti, C.J., Ras activity and cyclin D1 expression: an essential mechanism of mouse skin tumor development (1999) Mol. Carcinog., 24, pp. 1-6
  • Albanese, C., Johnson, J., Watanabe, G., Eklund, N., Vu, D., Arnold, A., Pestell, R.G., Transforming p21ras mutants and c-Ets-2 activate the cyclin D1 promoter through distinguishable regions (1995) J. Biol. Chem., 270, pp. 23589-23597
  • Sa, G., Stacey, D.W., Stacey P27 expression is regulated by separate signaling pathways, downstream of Ras, in each cell cycle phase (2004) Exp. Cell. Res., 300, pp. 427-439
  • Rivard, N., Boucher, M.J., Asselin, C., L'Allemain, G., MAP kinase cascade is required for p27 downregulation and S phase entry in fibroblasts and epithelial cells (1999) Am. J. Physiol., 277, pp. C652-C664
  • Larrea, M.D., Wander, S.A., Slingerland, J.M., P27 as Jekyll and Hyde: regulation of cell cycle and cell motility (2009) Cell Cycle, 8, pp. 3455-3461
  • Grimmler, M., Wang, Y., Mund, T., Cilensek, Z., Keidel, E.M., Waddell, M.B., Jäkel, H., Hengst, L., Cdk-inhibitory activity and stability of p27KIP1 are directly regulated by oncogenic tyrosine kinases (2007) Cell, 128, pp. 269-280
  • Chu, I., Sun, J., Arnaout, A., Kahn, H., Hanna, W., Narod, S., Sun, P., Slingerland, J., P27 phosphorylation by src regulates inhibition of cyclin E-Cdk2 (2007) Cell, 128, pp. 281-294
  • Viglietto, G., Motti, M.L., Bruni, P., Melillo, R.M., D'Alessio, A., Califano, D., Vinci, F., Santoro, M., Cytoplasmic relocalization and inhibition of the cyclin-dependent kinase inhibitor p27KIP1 by PKB/Akt-mediated phosphorylation in breast cancer (2002) Nat. Med., 8, pp. 1136-1144
  • Liang, J., Zubovitz, J., Petrocelli, T., Kotchetkov, R., Connor, M.K., Han, K., Lee, J.H., Slingerland, J.M., PKB/Akt phosphorylates p27, impairs nuclear import of p27 and opposes p27-mediated G1 arrest (2002) Nat. Med., 8, pp. 1153-1160
  • James, M.K., Ray, A., Leznova, D., Blain, S.W., Differential modification of p27KIP1 controls its cyclin D-cdk4 inhibitory activity (2008) Mol. Cell. Biol., 28, pp. 498-510

Citas:

---------- APA ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., Molinari, B.L.,..., Durán, H. (2011) . H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1. Cancer Letters, 305(1), 58-68.
http://dx.doi.org/10.1016/j.canlet.2011.02.026
---------- CHICAGO ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., et al. "H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1" . Cancer Letters 305, no. 1 (2011) : 58-68.
http://dx.doi.org/10.1016/j.canlet.2011.02.026
---------- MLA ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., et al. "H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1" . Cancer Letters, vol. 305, no. 1, 2011, pp. 58-68.
http://dx.doi.org/10.1016/j.canlet.2011.02.026
---------- VANCOUVER ----------
Ibañez, I.L., Policastro, L.L., Tropper, I., Bracalente, C., Palmieri, M.A., Rojas, P.A., et al. H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1. Cancer Lett. 2011;305(1):58-68.
http://dx.doi.org/10.1016/j.canlet.2011.02.026