Artículo

Vota, D.; Aguero, M.; Grasso, E.; Hauk, V.; Gallino, L.; Soczewski, E.; Pérez Leirós, C.; Ramhorst, R. "Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells" (2017) Molecular and Cellular Endocrinology. 443:146-154
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Abstract:

Trophoblast cells produce several inmmuneregulators like the Vasoactive Intestinal Peptide (VIP) and P4 targeting multiple circuits, and also display an intese phagocytic ability allowing embryo implantation in a tolerogenic context. Here, we explored whether P4 and VIP- crosstalk modulates trophoblast cell function, focus on the phagocytic ability and the immune homeostasis maintenance. P4 enhanced the phagocytosis in trophoblast-derived cells quantified by the engulfment of latex-beads or eryptotic erythrocytes. P4 and VIP modulated the balance of anti/pro-inflammatory mediators, increasing TGF-β expression, with no changes in IL-1, IL-6, or nitrites production. This modulation was accompained by transcription factor expression changes that could turn on tolerogenic programs represented by increased PPAR-γ and decreased IRF-5 expression. Finally, P4 stimulated VPAC2 expression in trophoblast cells and VPAC2 over-expression enhanced phagocytosis mimicking P4-effect. Therefore, P4 and VIP network enhances the phagocytic ability of trophoblast-derived cells, through a mechanism involving VPAC2 accompained with an anti-inflammatory context. © 2017 Elsevier B.V.

Registro:

Documento: Artículo
Título:Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells
Autor:Vota, D.; Aguero, M.; Grasso, E.; Hauk, V.; Gallino, L.; Soczewski, E.; Pérez Leirós, C.; Ramhorst, R.
Filiación:Immunopharmacology Laboratory, School of Sciences, University of Buenos Aires, IQUIBICEN-CONICET, Argentina
Palabras clave:Maternal–placental interface; Phagocytosis; Progesterone; Trophoblast-derived cells; Vasoactive intestinal polypeptide; glyceraldehyde 3 phosphate dehydrogenase; interferon regulatory factor 5; interleukin 1; interleukin 1beta; interleukin 6; kruppel like factor 4; nitrite; peroxisome proliferator activated receptor gamma; progesterone; transforming growth factor beta; transforming growth factor beta1; vasoactive intestinal polypeptide; vasoactive intestinal polypeptide receptor 1; vasoactive intestinal polypeptide receptor 2; antiinflammatory agent; progesterone; transcription factor; vasoactive intestinal polypeptide; vasoactive intestinal polypeptide receptor 2; antiinflammatory activity; Article; controlled study; erythrocyte; gene overexpression; human; human cell; phagocytosis; protein expression; protein protein interaction; trophoblast; cell line; cytology; drug effects; metabolism; Anti-Inflammatory Agents; Cell Line; Humans; Phagocytosis; Progesterone; Receptors, Vasoactive Intestinal Peptide, Type II; Transcription Factors; Trophoblasts; Vasoactive Intestinal Peptide
Año:2017
Volumen:443
Página de inicio:146
Página de fin:154
DOI: http://dx.doi.org/10.1016/j.mce.2017.01.022
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:glyceraldehyde 3 phosphate dehydrogenase, 9001-50-7; nitrite, 14797-65-0; progesterone, 57-83-0; vasoactive intestinal polypeptide, 37221-79-7; Anti-Inflammatory Agents; Progesterone; Receptors, Vasoactive Intestinal Peptide, Type II; Transcription Factors; Vasoactive Intestinal Peptide
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v443_n_p146_Vota

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Citas:

---------- APA ----------
Vota, D., Aguero, M., Grasso, E., Hauk, V., Gallino, L., Soczewski, E., Pérez Leirós, C.,..., Ramhorst, R. (2017) . Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells. Molecular and Cellular Endocrinology, 443, 146-154.
http://dx.doi.org/10.1016/j.mce.2017.01.022
---------- CHICAGO ----------
Vota, D., Aguero, M., Grasso, E., Hauk, V., Gallino, L., Soczewski, E., et al. "Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells" . Molecular and Cellular Endocrinology 443 (2017) : 146-154.
http://dx.doi.org/10.1016/j.mce.2017.01.022
---------- MLA ----------
Vota, D., Aguero, M., Grasso, E., Hauk, V., Gallino, L., Soczewski, E., et al. "Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells" . Molecular and Cellular Endocrinology, vol. 443, 2017, pp. 146-154.
http://dx.doi.org/10.1016/j.mce.2017.01.022
---------- VANCOUVER ----------
Vota, D., Aguero, M., Grasso, E., Hauk, V., Gallino, L., Soczewski, E., et al. Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells. Mol. Cell. Endocrinol. 2017;443:146-154.
http://dx.doi.org/10.1016/j.mce.2017.01.022