Artículo

Grinman, D.Y.; Romorini, L.; Presman, D.M.; Rocha-Viegas, L.; Coso, O.A.; Davio, C.; Pecci, A. "Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells" (2016) Molecular and Cellular Endocrinology. 419:259-267
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Abstract:

Epidermal growth factor (EGF) has been suggested to play a key role in the maintenance of epithelial cell survival during lactation. Previously, we demonstrated that EGF dependent activation of PI3K pathway prevents apoptosis in confluent murine HC11 cells cultured under low nutrient conditions. The EGF protective effect is associated with increased levels of the antiapoptotic protein Bcl-XL. Here, we identify the EGF-dependent mechanism involved in cell survival that converges in the regulation of bcl-X expression by activated CREB. EGF induces Bcl-XL expression through activation of a unique bcl-X promoter, the P1; being not only the PI3K/AKT signaling pathway but also the increase in cAMP levels and the concomitant PKA/CREB activation necessary for both bcl-XL upregulation and apoptosis avoidance. Results presented in this work suggest the existence of a novel connection between the EGF receptor and the adenylate cyclase that would have an impact in preventing apoptosis under low nutrient conditions. © 2015 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
Autor:Grinman, D.Y.; Romorini, L.; Presman, D.M.; Rocha-Viegas, L.; Coso, O.A.; Davio, C.; Pecci, A.
Filiación:Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Güiraldes 2160, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Güiraldes 2160, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
IFIBYNE (UBA-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Güiraldes 2160, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
LIAN-CONICET, Fundación para la Lucha contra las Enfermedades Neurodegenerativas de la Infancia, Ruta 9, Km. 52, 5, Escobar, Provincia de Buenos Aires, B1625XAF, Argentina
Cátedra de Química Medicinal, Departamento de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
Instituto de Investigaciones Farmacológicas, ININFA-UBA-CONICET, C1113AAD, Junin 954, Ciudad Autónoma de Buenos Aires, Argentina
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Building 41, B602, 41 Library Drive, Bethesda, MD 20892, United States
Palabras clave:Apoptosis; Bcl-X; CAMP; CREB; EGF; Mammary epithelial cells; 3' 5' cyclic adenosine monophosphate; adenosine phosphate; adenylate cyclase; cyclic AMP responsive element binding protein; epidermal growth factor; phosphatidylinositol 3 kinase; protein bcl x; protein bcl xl; protein kinase B; unclassified drug; Bcl2l1 protein, mouse; Creb1 protein, mouse; culture medium; cyclic AMP; cyclic AMP responsive element binding protein; epidermal growth factor; protein bcl x; animal cell; apoptosis; Article; breast epithelium cell; cell death; cell survival; cell viability; controlled study; correlational study; enzyme activation; mouse; nonhuman; priority journal; promoter region; protein expression; protein function; protein protein interaction; signal transduction; upregulation; animal; cell culture technique; cell line; cell survival; chemistry; culture medium; cytology; drug effects; female; genetics; metabolism; pharmacology; udder; Animals; bcl-X Protein; Cell Culture Techniques; Cell Line; Cell Survival; Culture Media; Cyclic AMP; Cyclic AMP Response Element-Binding Protein; Epidermal Growth Factor; Female; Mammary Glands, Animal; Mice; Promoter Regions, Genetic; Signal Transduction
Año:2016
Volumen:419
Página de inicio:259
Página de fin:267
DOI: http://dx.doi.org/10.1016/j.mce.2015.10.026
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:adenosine phosphate, 61-19-8, 8063-98-7; adenylate cyclase, 9012-42-4; cyclic AMP responsive element binding protein, 130428-87-4, 130939-96-7; epidermal growth factor, 59459-45-9, 62229-50-9; phosphatidylinositol 3 kinase, 115926-52-8; protein bcl xl, 151033-38-4; protein kinase B, 148640-14-6; cyclic AMP, 60-92-4; bcl-X Protein; Bcl2l1 protein, mouse; Creb1 protein, mouse; Culture Media; Cyclic AMP; Cyclic AMP Response Element-Binding Protein; Epidermal Growth Factor
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v419_n_p259_Grinman

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Citas:

---------- APA ----------
Grinman, D.Y., Romorini, L., Presman, D.M., Rocha-Viegas, L., Coso, O.A., Davio, C. & Pecci, A. (2016) . Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells. Molecular and Cellular Endocrinology, 419, 259-267.
http://dx.doi.org/10.1016/j.mce.2015.10.026
---------- CHICAGO ----------
Grinman, D.Y., Romorini, L., Presman, D.M., Rocha-Viegas, L., Coso, O.A., Davio, C., et al. "Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells" . Molecular and Cellular Endocrinology 419 (2016) : 259-267.
http://dx.doi.org/10.1016/j.mce.2015.10.026
---------- MLA ----------
Grinman, D.Y., Romorini, L., Presman, D.M., Rocha-Viegas, L., Coso, O.A., Davio, C., et al. "Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells" . Molecular and Cellular Endocrinology, vol. 419, 2016, pp. 259-267.
http://dx.doi.org/10.1016/j.mce.2015.10.026
---------- VANCOUVER ----------
Grinman, D.Y., Romorini, L., Presman, D.M., Rocha-Viegas, L., Coso, O.A., Davio, C., et al. Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells. Mol. Cell. Endocrinol. 2016;419:259-267.
http://dx.doi.org/10.1016/j.mce.2015.10.026