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Abstract:

The platelet-derived growth factor (PDGF) system is crucial for blood vessel stability. In the present study, we evaluated whether PDGFs play a critical intraovarian survival role in gonadotropin-dependent folliculogenesis. We examined the effect of intrabursal administration of a selective platelet-derived growth factor receptor (PDGFR) inhibitor (AG1295) on follicular development, proliferation, apoptosis and blood vessel formation and stability in ovaries from rats treated with equine chorionic gonadotropin (eCG). The percentages of preantral follicles (PAFs) and early antral follicles (EAFs) were lower in AG1295-treated ovaries than in control ovaries (p < 0.01-0.05). The percentage of atretic follicles (AtrFs) increased in AG1295-treated ovaries compared to control (p < 0.05). The ovarian weight and estradiol concentrations were lower in AG1295-treated ovaries than in the control group (p < 0.01 and p < 0.05, respectively), whereas progesterone concentrations did not change. AG1295 decreased the proliferation index in EAFs (p < 0.05) and increased the percentage of nuclei positive for cleaved caspase-3 and apoptotic DNA fragmentation (p < 0.01-0.05). AG1295 increased the expression of Bax (p < 0.05) without changes in the expression of Bcl-2 protein. AG1295-treated ovaries increased the cleavage of caspase-8 (p < 0.05) and decreased AKT and BAD phosphorylation compared with control ovaries (p < 0.05). AG1295 caused a decrease not only in the endothelial cell area but also in the area of pericytes and vascular smooth muscle cells (VSMCs) in the ovary (p < 0.05). Our findings suggest that the local inhibition of PDGFs causes an increase in ovarian apoptosis through an imbalance in the ratio of antiapoptotic to proapoptotic proteins, thus leading a larger number of follicles to atresia. PDGFs could exert their mechanism of action through an autocrine/paracrine effect on granulosa and theca cells mediated by PDGFRs. In conclusion, these data clearly indicate that the PDGF system is necessary for follicular development induced by gonadotropins. © 2015 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Inhibition of platelet-derived growth factor (PDGF) receptor affects follicular development and ovarian proliferation, apoptosis and angiogenesis in prepubertal eCG-treated rats
Autor:Pascuali, N.; Scotti, L.; Abramovich, D.; Irusta, G.; Di Pietro, M.; Bas, D.; Tesone, M.; Parborell, F.
Filiación:Instituto de Biología y Medicina Experimental (IByME), CONICET, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Angiogenesis; Apoptosis; Folliculogenesis; Ovary; Platelet-derived growth factor; 6,7 dimethyl 2 phenylquinoxaline; caspase 3; caspase 8; chorionic gonadotropin; estradiol; phosphatidylinositol 3 kinase; platelet derived growth factor receptor; progesterone; protein BAD; protein Bax; protein bcl 2; protein kinase B; 6,7-dimethoxy-2-phenylquinoxaline; chorionic gonadotropin; platelet derived growth factor; platelet derived growth factor receptor; tyrphostin; angiogenesis; animal cell; animal experiment; animal tissue; apoptosis; Article; cell proliferation; cell survival; controlled study; DNA extraction; DNA fragmentation; endothelium cell; female; immunohistochemistry; morphology; nonhuman; organ weight; ovary; ovary follicle cell; ovary follicle development; pericyte; prepuberty; priority journal; protein expression; protein phosphorylation; radioimmunoassay; rat; vascular smooth muscle cell; Western blotting; angiogenesis; animal; antagonists and inhibitors; cytology; horse; ovary; ovary follicle; physiology; sexual maturation; Sprague Dawley rat; vascularization; Animals; Apoptosis; Cell Proliferation; Chorionic Gonadotropin; Female; Horses; Neovascularization, Physiologic; Ovarian Follicle; Ovary; Platelet-Derived Growth Factor; Rats, Sprague-Dawley; Receptors, Platelet-Derived Growth Factor; Sexual Maturation; Tyrphostins
Año:2015
Volumen:412
Página de inicio:148
Página de fin:158
DOI: http://dx.doi.org/10.1016/j.mce.2015.04.021
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:6,7 dimethyl 2 phenylquinoxaline, 71897-07-9; caspase 3, 169592-56-7; caspase 8; chorionic gonadotropin, 9002-61-3; estradiol, 50-28-2; phosphatidylinositol 3 kinase, 115926-52-8; progesterone, 57-83-0; protein bcl 2, 219306-68-0; protein kinase B, 148640-14-6; 6,7-dimethoxy-2-phenylquinoxaline; Chorionic Gonadotropin; Platelet-Derived Growth Factor; Receptors, Platelet-Derived Growth Factor; Tyrphostins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v412_n_p148_Pascuali

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Citas:

---------- APA ----------
Pascuali, N., Scotti, L., Abramovich, D., Irusta, G., Di Pietro, M., Bas, D., Tesone, M.,..., Parborell, F. (2015) . Inhibition of platelet-derived growth factor (PDGF) receptor affects follicular development and ovarian proliferation, apoptosis and angiogenesis in prepubertal eCG-treated rats. Molecular and Cellular Endocrinology, 412, 148-158.
http://dx.doi.org/10.1016/j.mce.2015.04.021
---------- CHICAGO ----------
Pascuali, N., Scotti, L., Abramovich, D., Irusta, G., Di Pietro, M., Bas, D., et al. "Inhibition of platelet-derived growth factor (PDGF) receptor affects follicular development and ovarian proliferation, apoptosis and angiogenesis in prepubertal eCG-treated rats" . Molecular and Cellular Endocrinology 412 (2015) : 148-158.
http://dx.doi.org/10.1016/j.mce.2015.04.021
---------- MLA ----------
Pascuali, N., Scotti, L., Abramovich, D., Irusta, G., Di Pietro, M., Bas, D., et al. "Inhibition of platelet-derived growth factor (PDGF) receptor affects follicular development and ovarian proliferation, apoptosis and angiogenesis in prepubertal eCG-treated rats" . Molecular and Cellular Endocrinology, vol. 412, 2015, pp. 148-158.
http://dx.doi.org/10.1016/j.mce.2015.04.021
---------- VANCOUVER ----------
Pascuali, N., Scotti, L., Abramovich, D., Irusta, G., Di Pietro, M., Bas, D., et al. Inhibition of platelet-derived growth factor (PDGF) receptor affects follicular development and ovarian proliferation, apoptosis and angiogenesis in prepubertal eCG-treated rats. Mol. Cell. Endocrinol. 2015;412:148-158.
http://dx.doi.org/10.1016/j.mce.2015.04.021