Gonzalez, B.; Ratner, L.D.; Scerbo, M.J.; Di Giorgio, N.P.; Poutanen, M.; Huhtaniemi, I.T.; Calandra, R.S.; Lux-Lantos, V.A.R.; Cambiasso, M.J.; Rulli, S.B. "Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens" (2014) Molecular and Cellular Endocrinology. 390(1-2):102-111
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Transgenic female mice overexpressing the α- and β. - subunits of human chorionic gonadotropin (hCGαβ+) exhibited precocious puberty, as evidenced by early vaginal opening. Chronically elevated hCG in 21-day-old hCGαβ+ females stimulated gonadal androgen production, which exerted negative feedback over the endogenous gonadotropin synthesis, and activated the hypothalamic GnRH pulsatility and gene expression. Transgenic females also exhibited elevated hypothalamic aromatization in the preoptic area (POA), which is the sexually-differentiated area that controls the LH surge in adulthood. Ovariectomy at 14. days of age was unable to rescue this phenotype. However, the blockade of androgen action by flutamide from postnatal day 6 onwards reduced the aromatase levels in the POA of hCGαβ+ females. Our results suggest that early exposure of females to androgen action during a critical period between postnatal days 6-14 induces sex-specific organizational changes of the brain, which affect the aromatase expression in the POA at the onset of precocious puberty. © 2014 Elsevier Ireland Ltd.


Documento: Artículo
Título:Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens
Autor:Gonzalez, B.; Ratner, L.D.; Scerbo, M.J.; Di Giorgio, N.P.; Poutanen, M.; Huhtaniemi, I.T.; Calandra, R.S.; Lux-Lantos, V.A.R.; Cambiasso, M.J.; Rulli, S.B.
Filiación:Instituto de Biología y Medicina Experimental-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Department of Physiology, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland
Turku Center for Disease Modeling, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland
Department of Surgery and Cancer, Imperial College London, London W12 0NN, United Kingdom
Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET, Universidad Nacional de Córdoba, Casilla de Correo 389, 5000 Córdoba, Argentina
Palabras clave:Aromatase; Human chorionic gonadotropin; Hypothalamic-pituitary-gonadal axis; Precocious puberty; Transgenic mice; androgen; aromatase; chorionic gonadotropin alpha subunit; chorionic gonadotropin beta subunit; flutamide; follitropin; gonadorelin; luteinizing hormone; antiandrogen; aromatase; chorionic gonadotropin; estradiol; flutamide; follitropin; gonadorelin; testosterone; androgen synthesis; animal cell; animal experiment; animal model; animal tissue; aromatization; article; controlled study; ex vivo study; female; fetus; gene expression; gonadotropin release; hormone determination; hypothalamus hypophysis gonad system; in vitro study; mouse; negative feedback; nonhuman; organ weight; precocious puberty; preoptic area; priority journal; sex difference; transgenic mouse; wild type; animal; blood; cell culture; human; hypophysis; hypothalamus; metabolism; pathophysiology; physiology; Puberty, Precocious; secretion (process); vagina; Androgen Antagonists; Animals; Aromatase; Cells, Cultured; Chorionic Gonadotropin; Estradiol; Female; Flutamide; Follicle Stimulating Hormone; Gene Expression; Gonadotropin-Releasing Hormone; Humans; Hypothalamus; Mice, Transgenic; Pituitary Gland; Puberty, Precocious; Testosterone; Vagina
Página de inicio:102
Página de fin:111
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
CAS:aromatase, 9039-48-9; flutamide, 13311-84-7; follitropin, 9002-68-0; gonadorelin, 33515-09-2, 9034-40-6; luteinizing hormone, 39341-83-8, 9002-67-9; chorionic gonadotropin, 9002-61-3; estradiol, 50-28-2; testosterone, 58-22-0; Androgen Antagonists; Aromatase; Chorionic Gonadotropin; Estradiol; Flutamide; Follicle Stimulating Hormone; Gonadotropin-Releasing Hormone; Testosterone


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---------- APA ----------
Gonzalez, B., Ratner, L.D., Scerbo, M.J., Di Giorgio, N.P., Poutanen, M., Huhtaniemi, I.T., Calandra, R.S.,..., Rulli, S.B. (2014) . Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens. Molecular and Cellular Endocrinology, 390(1-2), 102-111.
---------- CHICAGO ----------
Gonzalez, B., Ratner, L.D., Scerbo, M.J., Di Giorgio, N.P., Poutanen, M., Huhtaniemi, I.T., et al. "Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens" . Molecular and Cellular Endocrinology 390, no. 1-2 (2014) : 102-111.
---------- MLA ----------
Gonzalez, B., Ratner, L.D., Scerbo, M.J., Di Giorgio, N.P., Poutanen, M., Huhtaniemi, I.T., et al. "Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens" . Molecular and Cellular Endocrinology, vol. 390, no. 1-2, 2014, pp. 102-111.
---------- VANCOUVER ----------
Gonzalez, B., Ratner, L.D., Scerbo, M.J., Di Giorgio, N.P., Poutanen, M., Huhtaniemi, I.T., et al. Elevated hypothalamic aromatization at the onset of precocious puberty in transgenic female mice hypersecreting human chorionic gonadotropin: Effect of androgens. Mol. Cell. Endocrinol. 2014;390(1-2):102-111.