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Abstract:

Neonatal androgenization masculinizes the GH axis and thus may impact on liver gene regulation. Neonatal testosterone administration to female mice decreased (defeminized) female predominant GH-dependent liver gene expression (Hnf6, Adh1, Prlr, Cyp3a41) and did not modify male predominant genes (Cyp7b1, Cyp4a12, Slp). Female predominance of Cis mRNA, an inhibitor of episodic GH signaling pathway, was unaltered. At birth, Cyp7b1 promoter exhibited a higher methylation status in female livers, while the Hnf6 promoter was equally methylated in both sexes; no differences in gene expression were detected at this age. In adulthood, consistent with sex specific predominance, lower methylation status was determined for the Cyp7b1 promoter in males, and for the Hnf6 promoter in females, and this last difference was prevented by neonatal androgenization. Therefore, early steroid treatment or eventually endocrine disruptor exposure may alter methylation status and sexual dimorphic expression of liver genes, and consequently modify liver physiology in females. © 2013 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice
Autor:Ramirez, M.C.; Zubeldía-Brenner, L.; Wargon, V.; Ornstein, A.M.; Becu-Villalobos, D.
Filiación:Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, V. Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Cyps; DNA methylation; GH; Liver; Neonatal testosterone; Sexual differences; androgen; DNA; endocrine disruptor; growth hormone; messenger RNA; testosterone; animal experiment; animal tissue; article; Cyp7b1 gene; DNA methylation; female; gene expression; Hnf6 gene; liver; male; mouse; newborn; nonhuman; nucleotide sequence; priority journal; promoter region; sex difference; sex differentiation; sexual development; signal transduction; Cyps; DNA methylation; GH; Liver; Neonatal testosterone; Sexual differences; Androgens; Animals; Animals, Newborn; DNA Methylation; Female; Gene Expression Regulation, Developmental; Growth Hormone; Hepatocyte Nuclear Factor 6; Liver; Male; Mice; Promoter Regions, Genetic; Sex Characteristics; Signal Transduction; Steroid Hydroxylases; Testosterone; Virilism
Año:2014
Volumen:382
Número:2
Página de inicio:825
Página de fin:834
DOI: http://dx.doi.org/10.1016/j.mce.2013.11.003
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:DNA, 9007-49-2; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v382_n2_p825_Ramirez

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Citas:

---------- APA ----------
Ramirez, M.C., Zubeldía-Brenner, L., Wargon, V., Ornstein, A.M. & Becu-Villalobos, D. (2014) . Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice. Molecular and Cellular Endocrinology, 382(2), 825-834.
http://dx.doi.org/10.1016/j.mce.2013.11.003
---------- CHICAGO ----------
Ramirez, M.C., Zubeldía-Brenner, L., Wargon, V., Ornstein, A.M., Becu-Villalobos, D. "Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice" . Molecular and Cellular Endocrinology 382, no. 2 (2014) : 825-834.
http://dx.doi.org/10.1016/j.mce.2013.11.003
---------- MLA ----------
Ramirez, M.C., Zubeldía-Brenner, L., Wargon, V., Ornstein, A.M., Becu-Villalobos, D. "Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice" . Molecular and Cellular Endocrinology, vol. 382, no. 2, 2014, pp. 825-834.
http://dx.doi.org/10.1016/j.mce.2013.11.003
---------- VANCOUVER ----------
Ramirez, M.C., Zubeldía-Brenner, L., Wargon, V., Ornstein, A.M., Becu-Villalobos, D. Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice. Mol. Cell. Endocrinol. 2014;382(2):825-834.
http://dx.doi.org/10.1016/j.mce.2013.11.003