Artículo
Abstract:
Experimental evidence has demonstrated that multiple doses of LH will increase the steroidogenic capacity of Leydig cells. This work was undertaken with the aim of defining the effect of a second hCG administration on the desensitized state, measuring binding of the gonadotropin and the steroidogenic capacity of rat testes. A single injection of 200 IU hCG induced a sharp increase of plasma testosterone which was still evident 24 h later. A second peak was observed at 72 h. The in vivo refractoriness of Leydig cells between 24 and 72 h after the single injection was proved by the fact that a second administration of hCG, 2 h before sacrifice, did not induce any increase in plasma testosterone. A second administration of 200 IU hCG, 48 h after the first injection, showed a similar pattern but on the 5th day there was an increased stimulation of testosterone production with respect to that obtained after a single dose of hCG. The in vitro studies on testicular binding capacity and steroidogenic responsiveness showed that the second administration of hCG, 48 h after the first injection, maintained the testicular binding capacity at the lowest level and the 'adenylate cyclase desensitization' but restored the steroidogenic capacity to even supramaximal values, compared to normal rats, 3 days after this second hCG administration. These results would support a dissociation between receptor loss and maximal testosterone synthesis as well as possibly indicating an alternative pathway different from the classical. © 1984.
Registro:
| Documento: | Artículo |
| Título: | Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells |
| Autor: | Calvo, J.C.; Radicella, J.P.; Pignataro, O.P.; Charreau, E.H. |
| Filiación: | Instituto de Biologia y Medicina Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
| Palabras clave: | desensitization; hCG; testis; bucladesine; chorionic gonadotropin; cycloheximide; radioisotope; testosterone; animal cell; animal experiment; chorionic gonadotropin i 125; desensitization; drug efficacy; drug receptor binding; endocrine system; hydroxyprogesterone h 3; intramuscular drug administration; leydig cell; male genital system; nonhuman; pharmacokinetics; progesterone h 3; rat; steroidogenesis; testis; testosterone h 3; testosterone release; Animal; Bucladesine; Chorionic Gonadotropin; Cyclic AMP; Drug Resistance; Hydroxyprogesterones; Leydig Cells; Male; Progesterone; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; Testosterone |
| Año: | 1984 |
| Volumen: | 34 |
| Número: | 1 |
| Página de inicio: | 31 |
| Página de fin: | 38 |
| DOI: | http://dx.doi.org/10.1016/0303-7207(84)90156-4 |
| Título revista: | Molecular and Cellular Endocrinology |
| Título revista abreviado: | Mol. Cell. Endocrinol. |
| ISSN: | 03037207 |
| CODEN: | MCEND |
| CAS: | bucladesine, 16980-89-5, 362-74-3; chorionic gonadotropin, 9002-61-3; cycloheximide, 642-81-9, 66-81-9; testosterone, 58-22-0; Bucladesine, 362-74-3; Chorionic Gonadotropin; Cyclic AMP, 60-92-4; Hydroxyprogesterones; Progesterone, 57-83-0; Testosterone, 57-85-2 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v34_n1_p31_Calvo |
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Citas:
---------- APA ----------
Calvo, J.C., Radicella, J.P., Pignataro, O.P. & Charreau, E.H. (1984) . Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells. Molecular and Cellular Endocrinology, 34(1), 31-38.http://dx.doi.org/10.1016/0303-7207(84)90156-4
---------- CHICAGO ----------
Calvo, J.C., Radicella, J.P., Pignataro, O.P., Charreau, E.H. "Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells" . Molecular and Cellular Endocrinology 34, no. 1 (1984) : 31-38.http://dx.doi.org/10.1016/0303-7207(84)90156-4
---------- MLA ----------
Calvo, J.C., Radicella, J.P., Pignataro, O.P., Charreau, E.H. "Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells" . Molecular and Cellular Endocrinology, vol. 34, no. 1, 1984, pp. 31-38.http://dx.doi.org/10.1016/0303-7207(84)90156-4
---------- VANCOUVER ----------
Calvo, J.C., Radicella, J.P., Pignataro, O.P., Charreau, E.H. Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells. Mol. Cell. Endocrinol. 1984;34(1):31-38.http://dx.doi.org/10.1016/0303-7207(84)90156-4
