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Abstract:

The antagonism exerted by melatonin on the glucocorticoid response has been well established, being strongly dependent on the cellular context. Previously, we found that melatonin inhibits glucocorticoid receptor (GR) dissociation from the chaperone hetero-complex and nuclear translocation on mouse thymocytes. Here, by performing confocal fluorescence microscopy and the Number and Brightness assay we show that in newborn hamster kidney cells (BHK21) melatonin neither affects GR nuclear translocation nor GR homodimerization. Instead, co-immunoprecipitation studies suggest that physiological concentrations of melatonin impair GR interaction with the transcriptional intermediary factor 2 (TIF2). This melatonin effect was not blocked by the MT 1/MT 2 receptor antagonist luzindole. Curiously, luzindole behaved as an antiglucocorticoid per se by impairing the glucocorticoid-dependent MMTV-driven gene expression affecting neither GR translocation nor GR-TIF2 interaction. © 2011 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells
Autor:Presman, D.M.; Levi, V.; Pignataro, O.P.; Pecci, A.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
IBYME-CONICET, C1428ADN, Buenos Aires, Argentina
Palabras clave:Antagonism; Glucocorticoids; Luzindole; Melatonin; TIF2; cyclic AMP; glucocorticoid receptor; luzindole; melatonin; nuclear receptor coactivator 2; cyclic AMP; dexamethasone; glucocorticoid; glucocorticoid receptor; melatonin; melatonin receptor; nuclear receptor coactivator 2; tryptamine derivative; animal cell; article; cellular distribution; concentration response; controlled study; dimerization; down regulation; enzyme inhibition; fluorescence microscopy; gene expression regulation; hamster; homodimerization; immunoprecipitation; kidney cell culture; nerve cell; newborn; nonhuman; nuclear import; oligomerization; priority journal; promoter region; protein DNA interaction; protein domain; protein expression; protein localization; protein protein interaction; thymocyte; transcription initiation; active transport; animal; cell line; drug antagonism; drug effect; gene expression; genetics; metabolism; Mouse mammary tumor oncovirus; signal transduction; Active Transport, Cell Nucleus; Animals; Cell Line; Cricetinae; Cyclic AMP; Dexamethasone; Dimerization; Gene Expression; Glucocorticoids; Immunoprecipitation; Mammary Tumor Virus, Mouse; Melatonin; Microscopy, Fluorescence; Nuclear Receptor Coactivator 2; Receptors, Glucocorticoid; Receptors, Melatonin; Signal Transduction; Tryptamines
Año:2012
Volumen:349
Número:2
Página de inicio:214
Página de fin:221
DOI: http://dx.doi.org/10.1016/j.mce.2011.10.026
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:cyclic AMP, 60-92-4; luzindole, 117946-91-5; melatonin, 73-31-4; dexamethasone, 50-02-2; tryptamine derivative, 1019-45-0; Cyclic AMP, 60-92-4; Dexamethasone, 50-02-2; Glucocorticoids; Melatonin, 73-31-4; Nuclear Receptor Coactivator 2; Receptors, Glucocorticoid; Receptors, Melatonin; Tryptamines; luzindole, 117946-91-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v349_n2_p214_Presman

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Citas:

---------- APA ----------
Presman, D.M., Levi, V., Pignataro, O.P. & Pecci, A. (2012) . Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells. Molecular and Cellular Endocrinology, 349(2), 214-221.
http://dx.doi.org/10.1016/j.mce.2011.10.026
---------- CHICAGO ----------
Presman, D.M., Levi, V., Pignataro, O.P., Pecci, A. "Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells" . Molecular and Cellular Endocrinology 349, no. 2 (2012) : 214-221.
http://dx.doi.org/10.1016/j.mce.2011.10.026
---------- MLA ----------
Presman, D.M., Levi, V., Pignataro, O.P., Pecci, A. "Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells" . Molecular and Cellular Endocrinology, vol. 349, no. 2, 2012, pp. 214-221.
http://dx.doi.org/10.1016/j.mce.2011.10.026
---------- VANCOUVER ----------
Presman, D.M., Levi, V., Pignataro, O.P., Pecci, A. Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells. Mol. Cell. Endocrinol. 2012;349(2):214-221.
http://dx.doi.org/10.1016/j.mce.2011.10.026