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Pignataro, O.P.; Radicella, J.P.; Calvo, J.C.; Charreau, E.H. "Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis" (1983) Molecular and Cellular Endocrinology. 33(1):53-67
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Abstract:

The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983.

Registro:

Documento: Artículo
Título:Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
Autor:Pignataro, O.P.; Radicella, J.P.; Calvo, J.C.; Charreau, E.H.
Filiación:Instituto de Biologia y Medicina Experimental and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos AiresArgentina
Palabras clave:cholesterol biosynthesis; HMG-CoA reductase; Leydig cell; hydroxymethylglutaryl coenzyme a reductase; radioisotope; acetyl coenzyme a c 14; animal experiment; biological model; cholesterol synthesis; endocrine system; leydig cell; male genital system; mitochondrion; nonhuman; rat; Acetates; Acetyl Coenzyme A; Animal; Cell Compartmentation; Cholesterol; Hydroxymethylglutaryl CoA Reductases; Leydig Cells; Male; Mitochondria; Rats; Support, Non-U.S. Gov't
Año:1983
Volumen:33
Número:1
Página de inicio:53
Página de fin:67
DOI: http://dx.doi.org/10.1016/0303-7207(83)90056-4
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:hydroxymethylglutaryl coenzyme A reductase, 37250-24-1; Acetates; Acetyl Coenzyme A, 72-89-9; Cholesterol, 57-88-5; Hydroxymethylglutaryl CoA Reductases, EC 1.1.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro

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Citas:

---------- APA ----------
Pignataro, O.P., Radicella, J.P., Calvo, J.C. & Charreau, E.H. (1983) . Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis. Molecular and Cellular Endocrinology, 33(1), 53-67.
http://dx.doi.org/10.1016/0303-7207(83)90056-4
---------- CHICAGO ----------
Pignataro, O.P., Radicella, J.P., Calvo, J.C., Charreau, E.H. "Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis" . Molecular and Cellular Endocrinology 33, no. 1 (1983) : 53-67.
http://dx.doi.org/10.1016/0303-7207(83)90056-4
---------- MLA ----------
Pignataro, O.P., Radicella, J.P., Calvo, J.C., Charreau, E.H. "Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis" . Molecular and Cellular Endocrinology, vol. 33, no. 1, 1983, pp. 53-67.
http://dx.doi.org/10.1016/0303-7207(83)90056-4
---------- VANCOUVER ----------
Pignataro, O.P., Radicella, J.P., Calvo, J.C., Charreau, E.H. Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis. Mol. Cell. Endocrinol. 1983;33(1):53-67.
http://dx.doi.org/10.1016/0303-7207(83)90056-4