Artículo

Pagotto, M.A.; Roldán, M.L.; Pagotto, R.M.; Lugano, M.C.; Pisani, G.B.; Rogic, G.; Molinas, S.M.; Trumper, L.; Pignataro, O.P.; Monasterolo, L.A. "Localization and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1) in the adult rat kidney" (2011) Molecular and Cellular Endocrinology. 332(1-2):253-260
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Abstract:

Cumulative evidence demonstrated effective downstream metabolism of pregnenolone in renal tissue. The aim of this study was to evaluate the expression and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1), which converts cholesterol into pregnenolone, in adult rat kidney. Immunohistochemical labeling for CYP11A1 was observed in renal cortex and medulla, on structures identified as distal convoluted tubule and thick ascending limb of Henle's loop, respectively. Immunoblotting analysis corroborated the renal expression of the protein in inner mitochondrial membrane fractions. The incubation of isolated mitochondria with the membrane-permeant cholesterol analogue 22R-hydroxycholesterol resulted in efficient formation of pregnenolone, the immediate precursor for the synthesis of all the steroid hormones. The low progesterone production rate observed in these experiments suggested a poor activity of 3β-hydroxysteroid dehydrogenase enzyme in renal mitochondria. The steroidogenic acute regulatory protein (StAR), involved in the mitochondrial import of cholesterol, was detected in renal tissue at both mRNA and protein level. Immunostaining for StAR showed similar distribution to that observed for CYP11A1. The expression of StAR and CYP11A1 was found to be higher in medulla than in cortex. This enhanced expression of steroidogenesis-related proteins correlated with a greater pregnenolone synthesis rate and higher steroid hormones tissular content measured in medulla. In conclusion, we have established the expression and localization of StAR and CYP11A1 protein, the ability of synthesizing pregnenolone and a region-specific content of sex hormones in the adult rat kidney. These data clearly show that the kidney is a steroid hormones synthesizing organ. It is proposed that the existence in the kidney of complete steroidogenic machinery would respond to a physiological significance. © 2010 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Localization and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1) in the adult rat kidney
Autor:Pagotto, M.A.; Roldán, M.L.; Pagotto, R.M.; Lugano, M.C.; Pisani, G.B.; Rogic, G.; Molinas, S.M.; Trumper, L.; Pignataro, O.P.; Monasterolo, L.A.
Filiación:Instituto de Fisiología Experimental, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Área Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina
Laboratorio de Endocrinología Molecular y Transducción de Señales, Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina
Área Morfología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina
Área Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo de Investigaciones de la Universidad Nacional de Rosario (CIUNR), Argentina
Laboratorio de Endocrinología Molecular y Transducción de Señales, Instituto de Biología y Medicina Experimental-CONICET, FCEN-UBA, Buenos Aires, Argentina
Área Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Suipacha 531, 2002LRK Rosario, Santa Fe, Argentina
Palabras clave:CYP11A1; Kidney; Mitochondria; Pregnenolone; Steroidogenesis; cholesterol monooxygenase (side chain cleaving); messenger RNA; pregnenolone; steroidogenic acute regulatory protein; animal cell; animal tissue; article; cell isolation; cholesterol metabolism; cholesterol transport; controlled study; enzyme activity; enzyme localization; Henle loop; intracellular transport; kidney; kidney cortex; kidney distal tubule; kidney function; kidney medulla; kidney structure; male; mitochondrial membrane; nonhuman; priority journal; progesterone synthesis; protein expression; protein function; rat; steroidogenesis; tissue distribution; tissue level; Animals; Carrier Proteins; Cholesterol Side-Chain Cleavage Enzyme; Kidney; Male; Mitochondrial Membranes; Phosphoproteins; Pregnenolone; Progesterone; Rats; Rats, Wistar; Receptors, GABA-A; Testosterone
Año:2011
Volumen:332
Número:1-2
Página de inicio:253
Página de fin:260
DOI: http://dx.doi.org/10.1016/j.mce.2010.10.020
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:cholesterol monooxygenase (side chain cleaving), 37292-81-2; pregnenolone, 145-13-1; steroidogenic acute regulatory protein, 168183-61-7; Bzrp protein, rat, 141440-82-6; Carrier Proteins; Cholesterol Side-Chain Cleavage Enzyme, 1.14.15.6; Phosphoproteins; Pregnenolone, 145-13-1; Progesterone, 57-83-0; Receptors, GABA-A; Testosterone, 58-22-0; steroidogenic acute regulatory protein
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v332_n1-2_p253_Pagotto

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Citas:

---------- APA ----------
Pagotto, M.A., Roldán, M.L., Pagotto, R.M., Lugano, M.C., Pisani, G.B., Rogic, G., Molinas, S.M.,..., Monasterolo, L.A. (2011) . Localization and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1) in the adult rat kidney. Molecular and Cellular Endocrinology, 332(1-2), 253-260.
http://dx.doi.org/10.1016/j.mce.2010.10.020
---------- CHICAGO ----------
Pagotto, M.A., Roldán, M.L., Pagotto, R.M., Lugano, M.C., Pisani, G.B., Rogic, G., et al. "Localization and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1) in the adult rat kidney" . Molecular and Cellular Endocrinology 332, no. 1-2 (2011) : 253-260.
http://dx.doi.org/10.1016/j.mce.2010.10.020
---------- MLA ----------
Pagotto, M.A., Roldán, M.L., Pagotto, R.M., Lugano, M.C., Pisani, G.B., Rogic, G., et al. "Localization and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1) in the adult rat kidney" . Molecular and Cellular Endocrinology, vol. 332, no. 1-2, 2011, pp. 253-260.
http://dx.doi.org/10.1016/j.mce.2010.10.020
---------- VANCOUVER ----------
Pagotto, M.A., Roldán, M.L., Pagotto, R.M., Lugano, M.C., Pisani, G.B., Rogic, G., et al. Localization and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1) in the adult rat kidney. Mol. Cell. Endocrinol. 2011;332(1-2):253-260.
http://dx.doi.org/10.1016/j.mce.2010.10.020