Artículo

Sánchez-Jiménez, F.; Pérez-Pérez, A.; González-Yanes, C.; Najib, S.; Varone, C.L.; Sánchez-Margalet, V. "Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells" (2011) Molecular and Cellular Endocrinology. 332(1-2):221-227
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Abstract:

Leptin is produced in placenta where it has been found to be an important autocrine signal for trophoblastic growth during pregnancy, promoting antiapoptotic and trophic effects. Leptin receptor is present in trophoblastic cells and leptin may fully activate signaling. We have previously implicated the RNA-binding protein Sam68 in leptin signal transduction in immune cells. In the present work, we have studied the possible role of Sam68 in leptin receptor signaling in trophoblastic cells (JEG-3 cells). Leptin dose-dependently stimulated Sam68 phosphorylation in JEG-3 cells, as assessed by immunoprecipitation and immunoblot with anti-phosphotyrosine antibodies. As previously observed in other systems, tyrosine phosphorylation of Sam68 in response to leptin inhibits its RNA binding capacity. Besides, leptin stimulation dose-dependently increases Sam68 expression in JEG-3 cells, as assessed by quantitative PCR. Consistently, the amount of Sam68 protein is increased after 24. h of leptin stimulation of trophoblastic cells. In order to study the possible role of Sam68 on leptin receptor synthesis, we employed antisense strategy to knockdown the expression of Sam68. We have found that a decrease in Sam68 expression leads to a decrease in leptin receptor amount in JEG-3 cells, as assessed both by quantitative PCR and immunoblot.These results strongly suggest the participation of Sam68 in leptin receptor signaling in human trophoblastic cells, and therefore, Sam68 may mediate some of the leptin effects in placenta. © 2010 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells
Autor:Sánchez-Jiménez, F.; Pérez-Pérez, A.; González-Yanes, C.; Najib, S.; Varone, C.L.; Sánchez-Margalet, V.
Filiación:Department of Clinical Biochemistry, Virgen Macarena University Hospital, University of Seville, Av. Dr. Fedriani 3, Seville 41071, Spain
INSERM U858, Institut de Médecine Moléculaire de Rangueil, CHU Rangueil, Dpt Cancer/E16, Toulouse Cedex 4, France
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Leptin receptor; Placenta; Sam68; Signal transduction; leptin; leptin receptor; protein Sam68; RNA; article; cell activation; cell level; controlled study; dose response; down regulation; hormonal regulation; human; human cell; priority journal; protein expression; protein function; protein phosphorylation; protein RNA binding; protein synthesis; signal transduction; trophoblast; Adaptor Proteins, Signal Transducing; Cell Line; Choriocarcinoma; DNA-Binding Proteins; Female; Humans; Leptin; Phosphorylation; Placenta; Pregnancy; Receptors, Leptin; RNA-Binding Proteins; Signal Transduction; Trophoblasts; Tyrosine
Año:2011
Volumen:332
Número:1-2
Página de inicio:221
Página de fin:227
DOI: http://dx.doi.org/10.1016/j.mce.2010.10.014
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:RNA, 63231-63-0; Adaptor Proteins, Signal Transducing; DNA-Binding Proteins; KHDRBS1 protein, human; Leptin; RNA-Binding Proteins; Receptors, Leptin; Tyrosine, 55520-40-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v332_n1-2_p221_SanchezJimenez

Referencias:

  • Ahima, R.S., Osei, S.Y., Leptin signaling (2004) Physiol. Behav., 81, pp. 223-241
  • Barlat, I., Maurier, F., Duchesne, M., Guitard, E., Tocque, B., Schweighoffer, F., A role for Sam68 in cell cycle progression antagonized by a spliced variant within the KH domain (1997) J. Biol. Chem., 272, pp. 3129-3132
  • Bifulco, G., Di Carlo, C., Caruso, M., Oriente, F., Di Spiezio Sardo, A., Formisano, P., Beguinot, F., Nappi, C., Glucose regulates insulin mitogenic effect by modulating SHP-2 activation and localization in JAr cells (2002) J. Biol. Chem., 277, pp. 24306-24314
  • Bjorbaek, C., Uotani, S., da Silva, B., Flier, J.S., Divergent signaling capacities of the long and short isoforms of the leptin receptor (1997) J. Biol. Chem., 272, pp. 32686-32695
  • Boileau, P., Cauzac, M., Pereira, M.A., Girard, J., Hauguel-De Mouzon, S., Dissociation between insulin-mediated signaling pathways and biological effects in placental cells: role of protein kinase B and MAPK phosphorylation (2001) Endocrinology, 142, pp. 3974-3979
  • Busa, R., Paronetto, M.P., Farini, D., Pierantozzi, E., Botti, F., Angelini, D.F., Attisani, F., Sette, C., The RNA-binding protein Sam68 contributes to proliferation and survival of human prostate cancer cells (2007) Oncogene, 26, pp. 4372-4382
  • Chomczynski, P., A reagent for the single-step simultaneous isolation of RNA DNA and proteins from cell and tissue samples (1993) BioTechniques, 15. , 532-4, 536-7
  • Coyle, J.H., Guzik, B.W., Bor, Y.C., Jin, L., Eisner-Smerage, L., Taylor, S.J., Rekosh, D., Hammarskjold, M.L., Sam68 enhances the cytoplasmic utilization of intron-containing RNA and is functionally regulated by the nuclear kinase Sik/BRK (2003) Mol. Cell. Biol., 23, pp. 92-103
  • Derry, J.J., Richard, S., Valderrama Carvajal, H., Ye, X., Vasioukhin, V., Cochrane, A.W., Chen, T., Tyner, A.L., Sik (BRK) phosphorylates Sam68 in the nucleus and negatively regulates its RNA binding ability (2000) Mol. Cell. Biol., 20, pp. 6114-6126
  • Edvinsson, L., Cerebrovascular endothelin receptor upregulation in cerebral ischemia (2009) Curr. Vasc. Pharmacol., 7, pp. 26-33
  • Flier, J.S., The adipocyte: storage depot or node on the energy information superhighway? (1995) Cell, 80, pp. 15-18
  • Fumagalli, S., Totty, N.F., Hsuan, J.J., Courtneidge, S.A., A target for Src in mitosis (1994) Nature, 368, pp. 871-874
  • Gambino, Y.P., Maymo, J.L., Perez-Perez, A., Duenas, J.L., Sanchez-Margalet, V., Calvo, J.C., Varone, C.L., 17Beta-estradiol enhances leptin expression in human placental cells through genomic and nongenomic actions (2010) Biol. Reprod., 83, pp. 42-51
  • Ghilardi, N., Skoda, R.C., The leptin receptor activates janus kinase 2 and signals for proliferation in a factor-dependent cell line (1997) Mol. Endocrinol., 11, pp. 393-399
  • Gorla, L., Cantu, M., Micciche, F., Patelli, C., Mondellini, P., Pierotti, M.A., Bongarzone, I., RET oncoproteins induce tyrosine phosphorylation changes of proteins involved in RNA metabolism (2006) Cell. Signal., 18, pp. 2272-2282
  • Haegebarth, A., Heap, D., Bie, W., Derry, J.J., Richard, S., Tyner, A.L., The nuclear tyrosine kinase BRK/Sik phosphorylates and inhibits the RNA-binding activities of the Sam68-like mammalian proteins SLM-1 and SLM-2 (2004) J. Biol. Chem., 279, pp. 54398-54404
  • Hauguel-de Mouzon, S., Lepercq, J., Catalano, P., The known and unknown of leptin in pregnancy (2006) Am. J. Obstet. Gynecol., 194, pp. 1537-1545
  • Lee, G.H., Proenca, R., Montez, J.M., Carroll, K.M., Darvishzadeh, J.G., Lee, J.I., Friedman, J.M., Abnormal splicing of the leptin receptor in diabetic mice (1996) Nature, 379, pp. 632-635
  • Li, Q.H., Haga, I., Shimizu, T., Itoh, M., Kurosaki, T., Fujisawa, J., Retardation of the G2-M phase progression on gene disruption of RNA binding protein Sam68 in the DT40 cell line (2002) FEBS Lett., 525, pp. 145-150
  • Li, R.H., Yu, M.M., Cheung, A.N., Wong, Y.F., Expression of leptin and leptin receptors in gestational trophoblastic diseases (2004) Gynecol. Oncol., 95, pp. 299-306
  • Lollmann, B., Gruninger, S., Stricker-Krongrad, A., Chiesi, M., Detection and quantification of the leptin receptor splice variants Ob-Ra, b, and, e in different mouse tissues (1997) Biochem. Biophys. Res. Commun., 238, pp. 648-652
  • Lukong, K.E., Richard, S., Sam68, the KH domain-containing superSTAR (2003) Biochim. Biophys. Acta, 1653, pp. 73-86
  • Magarinos, M.P., Sanchez-Margalet, V., Kotler, M., Calvo, J.C., Varone, C.L., Leptin promotes cell proliferation and survival of trophoblastic cells (2007) Biol. Reprod., 76, pp. 203-210
  • Maroni, P., Citterio, L., Piccoletti, R., Bendinelli, P., Sam68 and ERKs regulate leptin-induced expression of OB-Rb mRNA in C2C12 myotubes (2009) Mol. Cell. Endocrinol., 309, pp. 26-31
  • Martin-Romero, C., Sanchez-Margalet, V., Human leptin activates PI3K and MAPK pathways in human peripheral blood mononuclear cells: possible role of Sam68 (2001) Cell. Immunol., 212, pp. 83-91
  • Martin-Romero, C., Santos-Alvarez, J., Goberna, R., Sanchez-Margalet, V., Human leptin enhances activation and proliferation of human circulating T lymphocytes (2000) Cell. Immunol., 199, pp. 15-24
  • Maymo, J.L., Perez Perez, A., Sanchez-Margalet, V., Duenas, J.L., Calvo, J.C., Varone, C.L., Up-regulation of placental leptin by human chorionic gonadotropin (2009) Endocrinology, 150, pp. 304-313
  • Montezano, A.C., Burger, D., Paravicini, T.M., Chignalia, A.Z., Yusuf, H., Almasri, M., He, Y., Touyz, R.M., Nicotinamide adenine dinucleotide phosphate reduced oxidase 5 (Nox5) regulation by angiotensin II and endothelin-1 is mediated via calcium/calmodulin-dependent, rac-1-independent pathways in human endothelial cells (2010) Circ. Res., 106, pp. 1363-1373
  • Myers, M.G., Leptin receptor signaling and the regulation of mammalian physiology (2004) Recent Prog. Horm. Res., 59, pp. 287-304
  • Najib, S., Martin-Romero, C., Gonzalez-Yanes, C., Sanchez-Margalet, V., Role of Sam68 as an adaptor protein in signal transduction (2005) Cell Mol. Life Sci., 62, pp. 36-43
  • Najib, S., Sanchez-Margalet, V., Human leptin promotes survival of human circulating blood monocytes prone to apoptosis by activation of p42/44 MAPK pathway (2002) Cell. Immunol., 220, pp. 143-149
  • Najib, S., Sanchez-Margalet, V., Sam68 associates with the SH3 domains of Grb2 recruiting GAP to the Grb2-SOS complex in insulin receptor signaling (2002) J. Cell. Biochem., 86, pp. 99-106
  • Perez-Perez, A., Gambino, Y., Maymo, J., Goberna, R., Fabiani, F., Varone, C., Sanchez-Margalet, V., MAPK and PI3K activities are required for leptin stimulation of protein synthesis in human trophoblastic cells (2010) Biochem. Biophys. Res. Commun., 396, pp. 956-960
  • Perez-Perez, A., Maymo, J., Duenas, J.L., Goberna, R., Calvo, J.C., Varone, C., Sanchez-Margalet, V., Leptin prevents apoptosis of trophoblastic cells by activation of MAPK pathway (2008) Arch. Biochem. Biophys., 477, pp. 390-395
  • Perez-Perez, A., Maymo, J., Gambino, Y., Duenas, J.L., Goberna, R., Varone, C., Sanchez-Margalet, V., Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells (2009) Biol. Reprod., 81, pp. 826-832
  • Rajan, P., Gaughan, L., Dalgliesh, C., El-Sherif, A., Robson, C.N., Leung, H.Y., Elliott, D.J., The RNA-binding and adaptor protein Sam68 modulates signal-dependent splicing and transcriptional activity of the androgen receptor (2008) J. Pathol., 215, pp. 67-77
  • Rice, K.M., Garner, C.W., IGF-I regulates IRS-1 expression in 3T3-L1 adipocytes (1999) Biochem. Biophys. Res. Commun., 255, pp. 614-617
  • Sagawa, N., Yura, S., Itoh, H., Mise, H., Kakui, K., Korita, D., Takemura, M., Fujii, S., Role of leptin in pregnancy - a review (2002) Placenta, 23 (SUPPL. 1), pp. S80-S86
  • Sahu, A., Leptin signaling in the hypothalamus: emphasis on energy homeostasis and leptin resistance (2003) Front. Neuroendocrinol., 24, pp. 225-253
  • Sanchez-Margalet, V., Gonzalez-Yanes, C., Najib, S., Fernandez-Santos, J.M., Martin-Lacave, I., The expression of Sam68, a protein involved in insulin signal transduction, is enhanced by insulin stimulation (2003) Cell Mol. Life Sci., 60, pp. 751-758
  • Sanchez-Margalet, V., Martin-Romero, C., Human leptin signaling in human peripheral blood mononuclear cells: activation of the JAK-STAT pathway (2001) Cell. Immunol., 211, pp. 30-36
  • Sanchez-Margalet, V., Martin-Romero, C., Santos-Alvarez, J., Goberna, R., Najib, S., Gonzalez-Yanes, C., Role of leptin as an immunomodulator of blood mononuclear cells: mechanisms of action (2003) Clin. Exp. Immunol., 133, pp. 11-19
  • Sanchez-Margalet, V., Najib, S., Sam68 is a docking protein linking GAP and PI3K in insulin receptor signaling (2001) Mol. Cell. Endocrinol., 183, pp. 113-121
  • Sanchez-Margalet, V., Najib, S., P68 Sam is a substrate of the insulin receptor and associates with the SH2 domains of p85 PI3K (1999) FEBS Lett., 455, pp. 307-310
  • Santos-Alvarez, J., Goberna, R., Sanchez-Margalet, V., Human leptin stimulates proliferation and activation of human circulating monocytes (1999) Cell. Immunol., 194, pp. 6-11
  • Sette, C., Messina, V., Paronetto, M.P., Sam68: a new STAR in the male fertility firmament (2010) J. Androl., 31, pp. 66-74
  • Smith, J.T., Waddell, B.J., Leptin receptor expression in the rat placenta: changes in ob-ra, ob-rb, and ob-re with gestational age and suppression by glucocorticoids (2002) Biol. Reprod., 67, pp. 1204-1210
  • Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., Measurement of protein using bicinchoninic acid (1985) Anal. Biochem., 150, pp. 76-85
  • Standley, P.R., Standley, C.A., Identification of a functional Na+/Mg2+ exchanger in human trophoblast cells (2002) Am. J. Hypertens., 15, pp. 565-570
  • Sweeney, G., Leptin signalling (2002) Cell. Signal., 14, pp. 655-663
  • Tartaglia, L.A., The leptin receptor (1997) J. Biol. Chem., 272, pp. 6093-6096
  • Tartaglia, L.A., Dembski, M., Weng, X., Deng, N., Culpepper, J., Devos, R., Richards, G.J., Tepper, R.I., Identification and expression cloning of a leptin receptor, OB-R (1995) Cell, 83, pp. 1263-1271
  • Taylor, S.J., Resnick, R.J., Shalloway, D., Sam68 exerts separable effects on cell cycle progression and apoptosis (2004) BMC Cell Biol., 5, p. 5
  • Taylor, S.J., Shalloway, D., An RNA-binding protein associated with Src through its SH2 and SH3 domains in mitosis (1994) Nature, 368, pp. 867-871
  • Wang, L.L., Richard, S., Shaw, A.S., P62 association with RNA is regulated by tyrosine phosphorylation (1995) J. Biol. Chem., 270, pp. 2010-2013
  • Yamashita, T., Murakami, T., Otani, S., Kuwajima, M., Shima, K., Leptin receptor signal transduction: OBRa and OBRb of fa type (1998) Biochem. Biophys. Res. Commun., 246, pp. 752-759
  • Zhang, M., Guller, S., Huang, Y., Method to enhance transfection efficiency of cell lines and placental fibroblasts (2007) Placenta, 28, pp. 779-782
  • Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., Friedman, J.M., Positional cloning of the mouse obese gene and its human homologue (1994) Nature, 372, pp. 425-432
  • Zygmunt, M., Hahn, D., Kiesenbauer, N., Munstedt, K., Lang, U., Invasion of cytotrophoblastic (JEG-3) cells is up-regulated by interleukin-15 in vitro (1998) Am. J. Reprod. Immunol., 40, pp. 326-331

Citas:

---------- APA ----------
Sánchez-Jiménez, F., Pérez-Pérez, A., González-Yanes, C., Najib, S., Varone, C.L. & Sánchez-Margalet, V. (2011) . Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells. Molecular and Cellular Endocrinology, 332(1-2), 221-227.
http://dx.doi.org/10.1016/j.mce.2010.10.014
---------- CHICAGO ----------
Sánchez-Jiménez, F., Pérez-Pérez, A., González-Yanes, C., Najib, S., Varone, C.L., Sánchez-Margalet, V. "Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells" . Molecular and Cellular Endocrinology 332, no. 1-2 (2011) : 221-227.
http://dx.doi.org/10.1016/j.mce.2010.10.014
---------- MLA ----------
Sánchez-Jiménez, F., Pérez-Pérez, A., González-Yanes, C., Najib, S., Varone, C.L., Sánchez-Margalet, V. "Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells" . Molecular and Cellular Endocrinology, vol. 332, no. 1-2, 2011, pp. 221-227.
http://dx.doi.org/10.1016/j.mce.2010.10.014
---------- VANCOUVER ----------
Sánchez-Jiménez, F., Pérez-Pérez, A., González-Yanes, C., Najib, S., Varone, C.L., Sánchez-Margalet, V. Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells. Mol. Cell. Endocrinol. 2011;332(1-2):221-227.
http://dx.doi.org/10.1016/j.mce.2010.10.014