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Abstract:

Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). However, PKA-independent cAMP signaling has also been described. In this paper, it was found that H89, an inhibitor of PKA, was able to block MCE but not differentiation of 3T3-L1 fibroblast. Consistently, MCE did not occur in the absence of MIX in the differentiation mixture but was recovered by overexpression of a catalytic subunit of PKA. In addition, the transfection of 3T3-L1 fibroblast with a dominant-negative mutant of PKA inhibited MCE. On the other hand, differentiation of 3T3-L1 fibroblast to adipocytes did not occur when MIX was not present in the differentiation mixture and it could not be recovered by overexpression of a catalytic subunit of PKA. Differentiation was restored by addition of either dibutyryl-cAMP (db-cAMP) or 8 CPT-2 Me-cAMP. The latter activates cAMP-EPAC but not PKA signaling. These results indicate that cAMP-PKA-independent signaling, is required for 3T3-L1 fibroblasts differentiation to adipocytes and MIX signaling through cAMP-PKA is necessary for MCE, although MCE is not essential for adipogenesis. © 2008 Elsevier Ireland Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
Autor:Martini, C.N.; Plaza, M.V.; Vila, M.d.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:3T3-L1 fibroblast; Adipogenesis; cAMP; Insulin; Methylisobutylxanthine (MIX); Mitotic clonal expansion; PKA; 8 (4 chlorophenylthio) 2' O methyladenosine 3',5' cyclic monphosphate; bucladesine; cyclic AMP; cyclic AMP dependent protein kinase; n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide; unclassified drug; adipocyte; animal cell; article; catalysis; cell differentiation; fibroblast; genetic transfection; mouse; nonhuman; priority journal; protein expression; 1-Methyl-3-isobutylxanthine; 3T3-L1 Cells; Adipogenesis; Animals; Cell Differentiation; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Dexamethasone; Fibroblasts; Insulin; Isoquinolines; Mice; Mitosis; Protein Kinase Inhibitors; Signal Transduction; Sulfonamides; Theophylline
Año:2009
Volumen:298
Número:1-2
Página de inicio:42
Página de fin:47
DOI: http://dx.doi.org/10.1016/j.mce.2008.10.023
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:bucladesine, 16980-89-5, 362-74-3; cyclic AMP, 60-92-4; n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide, 127243-85-0; 1-Methyl-3-isobutylxanthine, 28822-58-4; 8-cyclopentyl-1,3-dimethylxanthine, 35873-49-5; Cyclic AMP, 60-92-4; Cyclic AMP-Dependent Protein Kinases, EC 2.7.11.11; Dexamethasone, 50-02-2; H 89, 127243-85-0; Insulin, 11061-68-0; Isoquinolines; Protein Kinase Inhibitors; Sulfonamides; Theophylline, 58-55-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v298_n1-2_p42_Martini

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Citas:

---------- APA ----------
Martini, C.N., Plaza, M.V. & Vila, M.d.C. (2009) . PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation. Molecular and Cellular Endocrinology, 298(1-2), 42-47.
http://dx.doi.org/10.1016/j.mce.2008.10.023
---------- CHICAGO ----------
Martini, C.N., Plaza, M.V., Vila, M.d.C. "PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation" . Molecular and Cellular Endocrinology 298, no. 1-2 (2009) : 42-47.
http://dx.doi.org/10.1016/j.mce.2008.10.023
---------- MLA ----------
Martini, C.N., Plaza, M.V., Vila, M.d.C. "PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation" . Molecular and Cellular Endocrinology, vol. 298, no. 1-2, 2009, pp. 42-47.
http://dx.doi.org/10.1016/j.mce.2008.10.023
---------- VANCOUVER ----------
Martini, C.N., Plaza, M.V., Vila, M.d.C. PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation. Mol. Cell. Endocrinol. 2009;298(1-2):42-47.
http://dx.doi.org/10.1016/j.mce.2008.10.023