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Abstract:

The effects of prostaglandins (PGs) on rat pineal metabolism were examined in vitro. PGE2 (0.01-1 μM) increased the activity of serotonin-N-acetyltransferase (SNAT), the stimulation curve exhibiting a maximum at 0.1 μM. PGE1 increased SNAT activity only at the highest dose (1 μm) whereas PGF2α, 15-keto-PGF2α or PGI2 did not affect the enzymic activity. The stimulation of SNAT activity brought about by PGE2 in pineals from ganglionectomized rats was greater than in sham-operated controls at all the doses studied, suggesting that the observed effect is predominantly post-synaptic. Only PGE2 significantly increased pineal cAMP accumulation in vitro at doses between 0.01 and 1 μm, and depressed the unoccupied cAMP-binding sites in pineal 900 g supernatants. The total number of cAMP-binding sites remained unaltered after incubation of PGE2. The present observations together with the previously reported NE-induced release of PGs in incubated pineal glands, the occurrence of pineal PG-binding sites and the indomethacin blockade of the nocturnal rise of pineal SNAT and melatonin content, support a role for PGs in the control of melatonin synthesis. © 1981.

Registro:

Documento: Artículo
Título:Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro
Autor:Ritta, M.N.; Cardinali, D.P.
Filiación:Centro de Estudios Farmacológicos y de Principles Naturales (CEFAPRIN), Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:cAMP; cAMP-binding sites; melatonin synthesis; pineal; prostaglandins; acyltransferase; arylamine acetyltransferase; cyclic AMP; prostaglandin derivative; prostaglandin E; prostaglandin E2; aralkylamine acetyltransferase; arylamine acetyltransferase; cyclic amp; drug receptor; prostacyclin; prostaglandin e2; prostaglandin f2 alpha; radioisotope; animal; article; binding site; comparative study; drug effect; enzyme activation; in vitro study; male; metabolism; pineal body; rat; rat strain; 15 oxoprostaglandin f2 alpha; acetyl coenzyme a c 14; animal experiment; cyclic amp h 3; dose response; drug response; endocrine system; pineal body; Acetyltransferases; Animal; Arylamine N-Acetyltransferase; Binding Sites; Comparative Study; Cyclic AMP; Dinoprostone; Enzyme Activation; In Vitro; Male; Pineal Gland; Prostaglandins E; Prostaglandins, Synthetic; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't
Año:1981
Volumen:23
Número:2
Página de inicio:151
Página de fin:159
DOI: http://dx.doi.org/10.1016/0303-7207(81)90066-6
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:acyltransferase, 9012-30-0, 9054-54-0; arylamine acetyltransferase, 9027-33-2; cyclic AMP, 60-92-4; prostaglandin E, 11042-70-9; prostaglandin E2, 363-24-6; aralkylamine acetyltransferase, 92941-56-5; prostacyclin, 35121-78-9, 61849-14-7; prostaglandin F2 alpha, 551-11-1; Acetyltransferases, EC 2.3.1.; Arylamine N-Acetyltransferase, EC 2.3.1.5; Cyclic AMP, 60-92-4; Dinoprostone, 363-24-6; Prostaglandins E; Prostaglandins, Synthetic
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v23_n2_p151_Ritta

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Citas:

---------- APA ----------
Ritta, M.N. & Cardinali, D.P. (1981) . Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro. Molecular and Cellular Endocrinology, 23(2), 151-159.
http://dx.doi.org/10.1016/0303-7207(81)90066-6
---------- CHICAGO ----------
Ritta, M.N., Cardinali, D.P. "Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro" . Molecular and Cellular Endocrinology 23, no. 2 (1981) : 151-159.
http://dx.doi.org/10.1016/0303-7207(81)90066-6
---------- MLA ----------
Ritta, M.N., Cardinali, D.P. "Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro" . Molecular and Cellular Endocrinology, vol. 23, no. 2, 1981, pp. 151-159.
http://dx.doi.org/10.1016/0303-7207(81)90066-6
---------- VANCOUVER ----------
Ritta, M.N., Cardinali, D.P. Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro. Mol. Cell. Endocrinol. 1981;23(2):151-159.
http://dx.doi.org/10.1016/0303-7207(81)90066-6