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Juvenal, G.J.; Pisarev, M.A.; Kleiman de Pisarev, D.L.; Altschuler, N. "Uptake, metabolism and action of triiodothyronine in calf-thyroid slices" (1981) Molecular and Cellular Endocrinology. 22(1):31-40
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Abstract:

T3 and T4 at 10-6M caused a significant inhibition on [3H]uridine incorporation into RNA in calf-thyroid slices. This effect was not altered by addition of 10-3M PTU or MMI. The metabolism of 125I-T3 was studied under the same conditions. There was a very slight dehalogenation after 60 min (less than 5%) which was inhibited by PTU. The time course of the uptake of labeled T3 showed a temperature dependence, and this uptake was not altered by 10-6M KI, 1-T4, DIT or MIT, thus indicating specificity. The early phase of labeled T3 entrance into the cell was inhibited by the addition of cold T3 in a dose-dependent manner from 10-9 to 10-5M. Slices previously stimulated by cAMP or cGMP showed a significant increase in the uptake of labeled T3. ATPase activity or ATP, protein or RNA synthesis does not seem to play a role in this process. The relationship between substitutions in the thyronine molecule and its biological action on RNA synthesis was also studied, and some preliminary conclusions were drawn. These studies demonstrate that T3 has a direct action on the thyroid. © 1981.

Registro:

Documento: Artículo
Título:Uptake, metabolism and action of triiodothyronine in calf-thyroid slices
Autor:Juvenal, G.J.; Pisarev, M.A.; Kleiman de Pisarev, D.L.; Altschuler, N.
Filiación:División Bioquímica Nuclear, Depto. Applicaciones Biológicas, Comisión Nacional de Energía Atómica; and Depto. de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:autoregulation; RNA synthesis; thyroid; triiodothyronine; adenosine triphosphatase; cyclic amp; cyclic gmp; cycloheximide; dactinomycin; diiodotyrosine; iodotyrosine; liothyronine; liothyronine i 125; ouabain; propylthiouracil; radioisotope; thiamazole; thyroxine; triiodothyronine derivative; animal experiment; autoregulation; calf; cattle; dose response; drug absorption; drug comparison; drug inhibition; drug metabolism; drug response; endocrine system; furomycin; in vitro study; rna synthesis; short survey; thyroid gland; uridine h 3; Animal; Cattle; Methimazole; Propylthiouracil; RNA; Structure-Activity Relationship; Support, Non-U.S. Gov't; Thyroid Gland; Thyroid Hormones; Thyroxine; Triiodothyronine
Año:1981
Volumen:22
Número:1
Página de inicio:31
Página de fin:40
DOI: http://dx.doi.org/10.1016/0303-7207(81)90100-3
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:adenosine triphosphatase, 37289-25-1, 9000-83-3; cyclic AMP, 60-92-4; cyclic GMP, 7665-99-8; cycloheximide, 642-81-9, 66-81-9; dactinomycin, 1402-38-6, 1402-58-0, 50-76-0; diiodotyrosine, 66-02-4; iodotyrosine, 3078-39-5, 70-78-0; liothyronine i 125, 24359-14-6; liothyronine, 6138-47-2, 6893-02-3; ouabain, 11018-89-6, 630-60-4; propylthiouracil, 51-52-5; thiamazole, 60-56-0; thyroxine, 7488-70-2; Methimazole, 60-56-0; Propylthiouracil, 51-52-5; RNA, 63231-63-0; Thyroid Hormones; Thyroxine, 7488-70-2; Triiodothyronine, 6893-02-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v22_n1_p31_Juvenal

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Citas:

---------- APA ----------
Juvenal, G.J., Pisarev, M.A., Kleiman de Pisarev, D.L. & Altschuler, N. (1981) . Uptake, metabolism and action of triiodothyronine in calf-thyroid slices. Molecular and Cellular Endocrinology, 22(1), 31-40.
http://dx.doi.org/10.1016/0303-7207(81)90100-3
---------- CHICAGO ----------
Juvenal, G.J., Pisarev, M.A., Kleiman de Pisarev, D.L., Altschuler, N. "Uptake, metabolism and action of triiodothyronine in calf-thyroid slices" . Molecular and Cellular Endocrinology 22, no. 1 (1981) : 31-40.
http://dx.doi.org/10.1016/0303-7207(81)90100-3
---------- MLA ----------
Juvenal, G.J., Pisarev, M.A., Kleiman de Pisarev, D.L., Altschuler, N. "Uptake, metabolism and action of triiodothyronine in calf-thyroid slices" . Molecular and Cellular Endocrinology, vol. 22, no. 1, 1981, pp. 31-40.
http://dx.doi.org/10.1016/0303-7207(81)90100-3
---------- VANCOUVER ----------
Juvenal, G.J., Pisarev, M.A., Kleiman de Pisarev, D.L., Altschuler, N. Uptake, metabolism and action of triiodothyronine in calf-thyroid slices. Mol. Cell. Endocrinol. 1981;22(1):31-40.
http://dx.doi.org/10.1016/0303-7207(81)90100-3