Bussmann, U.A.; Lanuza, G.M.; Bussmann, L.E. "Activin and follistatin in rat mammary gland" (2004) Molecular and Cellular Endocrinology. 221(1-2):9-19
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Mammary gland morphogenesis and differentiation are mediated through the combined activities of systemic hormones and locally synthesized growth factors. Activin, a member of the transforming growth factor (TGF)-β superfamily, is known to regulate the growth and differentiation of several cell types. In the present study, we investigated the role of activin in rat mammary gland on different stages of development. We found that activin A in vitro inhibits the proliferation of isolated acini, and this effect increases with the development of the gland. This factor also produces in vitro an inhibition of the final differentiation of acini obtained from 19th day pregnant rats. We also report the expression of activin receptors IIA and IIB mRNA in whole rat mammary gland and acini, with decreased levels of expression of type IIA (in both compartments) and IIB (in acini) during pregnancy and lactogenesis. In addition, we show that activin βB-subunit mRNA decreases throughout pregnancy, and that the mRNA levels of follistatin (Fst) (its ligand protein) are high in cycling rats and at the beginning of pregnancy and diminish thereafter, having the acini higher levels of expression. Our data show that activin βB-subunit, follistatin and ActRIIA and IIB transcripts are expressed in rat mammary gland at appropriate times and locations during development, allowing an interplay that might regulate activin action on growth and differentiation of the gland. © 2004 Elsevier Ireland Ltd. All rights reserved.


Documento: Artículo
Título:Activin and follistatin in rat mammary gland
Autor:Bussmann, U.A.; Lanuza, G.M.; Bussmann, L.E.
Filiación:Inst. de Biol. Y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Depto. de Quím. Biol., Fac. de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Differentiation; Growth; Growth factors; Lactogenesis; Pregnancy; activin; activin A; activin receptor; activin receptor 2a; activin receptor 2b; follistatin; ligand; messenger RNA; myelopid; unclassified drug; acinar cell; animal experiment; article; cell differentiation; cell isolation; cell proliferation; controlled study; female; growth regulation; mammary gland; milk production; nonhuman; pregnancy; priority journal; protein expression; rat; Activin Receptors; Activins; Animals; Caseins; Cell Differentiation; Cell Proliferation; Female; Follistatin; Gene Expression Regulation; Inhibin-beta Subunits; Inhibins; Lactation; Mammary Glands, Animal; Pregnancy; Rats; Rats, Sprague-Dawley; RNA, Messenger; Transforming Growth Factor beta
Página de inicio:9
Página de fin:19
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
CAS:activin A, 104625-48-1; activin receptor, 146990-50-3; activin, 114949-22-3; follistatin, 117628-82-7; myelopid, 126338-68-9; activin A; Activin Receptors, EC; Activins, 104625-48-1; Caseins; Follistatin; inhibin beta A subunit; inhibin-alpha subunit; Inhibin-beta Subunits, 93443-12-0; Inhibins, 57285-09-3; RNA, Messenger; Transforming Growth Factor beta


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---------- APA ----------
Bussmann, U.A., Lanuza, G.M. & Bussmann, L.E. (2004) . Activin and follistatin in rat mammary gland. Molecular and Cellular Endocrinology, 221(1-2), 9-19.
---------- CHICAGO ----------
Bussmann, U.A., Lanuza, G.M., Bussmann, L.E. "Activin and follistatin in rat mammary gland" . Molecular and Cellular Endocrinology 221, no. 1-2 (2004) : 9-19.
---------- MLA ----------
Bussmann, U.A., Lanuza, G.M., Bussmann, L.E. "Activin and follistatin in rat mammary gland" . Molecular and Cellular Endocrinology, vol. 221, no. 1-2, 2004, pp. 9-19.
---------- VANCOUVER ----------
Bussmann, U.A., Lanuza, G.M., Bussmann, L.E. Activin and follistatin in rat mammary gland. Mol. Cell. Endocrinol. 2004;221(1-2):9-19.