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Abstract:

Human epididymal tubules from 9 patients undergoing orchidectomy were cultured for periods of up to 8 days with preservation of the histological structure of the tissue. Addition of androgens (testosterone, 5α-dihydrotestosterone and 5α-androstane-3α,17β-diol) significantly increased the epithelial height, the incorporation of [3H]amino acids and [3H]thymidine. The effect on protein synthesis was significant 48 h after the onset of treatment and was maximal after 3 days. The effect on DNA replication was maximal during the 3rd day of treatment. In both instances maximal activity was achieved at a concentration of 10-7 M of testosterone. Cyproterone acetate (10-5 M) was able to negate all effects of androgens. © 1981.

Registro:

Documento: Artículo
Título:The organ culture of human epididymal tubules and their response to androgens
Autor:Tezón, J.G.; Blaquier, J.A.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:androgen stimulation; human epididymis; protein synthesis; amino acid; androgen; androstane 3,17 diol; androstanediol; androstanolone; cyproterone; cyproterone acetate; drug derivative; male contraceptive agent; testosterone; thymidine; androgen; androgen receptor; androstanediol; androstanolone; cyproterone acetate; radioisotope; testosterone; adult; aged; article; drug effect; epididymis; human; male; metabolism; organ culture; amino acid h 3; dna replication; endocrine system; epididymis; human cell; in vitro study; kidney tubule; male genital system; normal human; protein synthesis; thymidine h 3; Adult; Aged; Amino Acids; Androgens; Androstane-3,17-diol; Contraceptive Agents, Male; Cyproterone; Cyproterone Acetate; Epididymis; Human; Male; Middle Age; Organ Culture; Stanolone; Support, Non-U.S. Gov't; Testosterone; Thymidine
Año:1981
Volumen:21
Número:3
Página de inicio:233
Página de fin:242
DOI: http://dx.doi.org/10.1016/0303-7207(81)90017-4
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:amino acid, 65072-01-7; androstanediol, 1852-53-5, 25126-76-5, 67332-38-1; androstanolone, 521-18-6; cyproterone acetate, 427-51-0; cyproterone, 2098-66-0; testosterone, 58-22-0; thymidine, 50-89-5; Amino Acids; Androgens; Androstane-3,17-diol, 25126-76-5; Contraceptive Agents, Male; Cyproterone Acetate, 427-51-0; Cyproterone, 2098-66-0; Stanolone, 521-18-6; Testosterone, 57-85-2; Thymidine, 50-89-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v21_n3_p233_Tezon

Referencias:

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Citas:

---------- APA ----------
Tezón, J.G. & Blaquier, J.A. (1981) . The organ culture of human epididymal tubules and their response to androgens. Molecular and Cellular Endocrinology, 21(3), 233-242.
http://dx.doi.org/10.1016/0303-7207(81)90017-4
---------- CHICAGO ----------
Tezón, J.G., Blaquier, J.A. "The organ culture of human epididymal tubules and their response to androgens" . Molecular and Cellular Endocrinology 21, no. 3 (1981) : 233-242.
http://dx.doi.org/10.1016/0303-7207(81)90017-4
---------- MLA ----------
Tezón, J.G., Blaquier, J.A. "The organ culture of human epididymal tubules and their response to androgens" . Molecular and Cellular Endocrinology, vol. 21, no. 3, 1981, pp. 233-242.
http://dx.doi.org/10.1016/0303-7207(81)90017-4
---------- VANCOUVER ----------
Tezón, J.G., Blaquier, J.A. The organ culture of human epididymal tubules and their response to androgens. Mol. Cell. Endocrinol. 1981;21(3):233-242.
http://dx.doi.org/10.1016/0303-7207(81)90017-4