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Kuchenbauer, F.; Hopfner, U.; Stalla, J.; Arzt, E.; Stalla, G.K.; Páez-Pereda, M. "Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells" (2001) Molecular and Cellular Endocrinology. 175(1-2):141-148
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Abstract:

The extracellular matrix (ECM) conveys signals through membrane receptors called integrins producing changes in cell morphology, proliferation, differentiation and apoptosis. Previous studies suggest that the ECM plays an important role in pituitary physiology and tumorigenesis. In the present work we studied for the first time the effects of fibronectin, laminin, collagen I and collagen IV on hormone secretion and cell proliferation in the corticotroph tumor cell line AtT-20 and in normal pituitary cells, examining the signal transduction mechanisms that mediate these effects. ACTH production in AtT-20 cells was inhibited by fibronectin, laminin and collagen I. A reporter construct with the POMC promoter showed similar results, indicating that the effects of the ECM take place at the level of POMC gene transcription. In contrast, ACTH production was not significantly altered in normal pituitary cells. AtT-20 cell proliferation was stimulated by collagen IV and fibronectin, but inhibited by collagen I and laminin. In parallel, the cell morphology was modified by the ECM. We found that the production of reactive oxygen species mediate the effects of laminin and collagen IV. On the other hand, the effect of fibronectin was mimicked by β1-integrin and Rho activation. These results show for the first time that the ECM controls ACTH biosynthesis and proliferation in corticotroph tumor cells and suggest a role for the ECM in corticotroph adenoma development. Copyright © 2001 Elsevier Science Ireland Ltd.

Registro:

Documento: Artículo
Título:Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells
Autor:Kuchenbauer, F.; Hopfner, U.; Stalla, J.; Arzt, E.; Stalla, G.K.; Páez-Pereda, M.
Filiación:Max Planck Institute of Psychiatry, Department of Endocrinology, Kraepelinstrasse 10, 80804 Munich, Germany
Laboratory Fisiología y Biología Molecular, FCEN, Universidad de Buenos Aires and CONICET, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:ACTH; Corticotroph cells; Extracellular matrix; Integrins; POMC; Reactive oxygen species; collagen type 1; collagen type 4; corticotropin; fibronectin; integrin; laminin; reactive oxygen metabolite; article; cell proliferation; cell structure; controlled study; corticotropin release; extracellular matrix; genetic transcription; hormone release; human; human cell; hypophysis cell; priority journal; signal transduction; tumor cell; Adrenocorticotropic Hormone; Animals; Cell Division; Collagen; Extracellular Matrix; Fibronectins; Laminin; Mice; Pituitary Neoplasms; Pro-Opiomelanocortin; Promoter Regions (Genetics); Transcription, Genetic; Tumor Cells, Cultured
Año:2001
Volumen:175
Número:1-2
Página de inicio:141
Página de fin:148
DOI: http://dx.doi.org/10.1016/S0303-7207(01)00390-2
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:Adrenocorticotropic Hormone, 9002-60-2; Collagen, 9007-34-5; Fibronectins; Laminin; Pro-Opiomelanocortin, 66796-54-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v175_n1-2_p141_Kuchenbauer

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Citas:

---------- APA ----------
Kuchenbauer, F., Hopfner, U., Stalla, J., Arzt, E., Stalla, G.K. & Páez-Pereda, M. (2001) . Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells. Molecular and Cellular Endocrinology, 175(1-2), 141-148.
http://dx.doi.org/10.1016/S0303-7207(01)00390-2
---------- CHICAGO ----------
Kuchenbauer, F., Hopfner, U., Stalla, J., Arzt, E., Stalla, G.K., Páez-Pereda, M. "Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells" . Molecular and Cellular Endocrinology 175, no. 1-2 (2001) : 141-148.
http://dx.doi.org/10.1016/S0303-7207(01)00390-2
---------- MLA ----------
Kuchenbauer, F., Hopfner, U., Stalla, J., Arzt, E., Stalla, G.K., Páez-Pereda, M. "Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells" . Molecular and Cellular Endocrinology, vol. 175, no. 1-2, 2001, pp. 141-148.
http://dx.doi.org/10.1016/S0303-7207(01)00390-2
---------- VANCOUVER ----------
Kuchenbauer, F., Hopfner, U., Stalla, J., Arzt, E., Stalla, G.K., Páez-Pereda, M. Extracellular matrix components regulate ACTH production and proliferation in corticotroph tumor cells. Mol. Cell. Endocrinol. 2001;175(1-2):141-148.
http://dx.doi.org/10.1016/S0303-7207(01)00390-2