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Abstract:

Changes in morphology and DNA synthesis in cultured myoblasts in response to 1,25-dihydroxy-vitamin D3 [1,25(OH)2D3] have previously suggested that the vitamin D hormone may affect muscle cell proliferation and differentiation. However, this interpretation was not substantiated by measurement of specific biochemical markers of myogenesis. To study the effect of 1,25(OH)2D3 on muscle development, chicken embryo myoblasts were cultured for 1-6 days in the presence or absence of 1,25(OH)2D3 (10-9 M). The hormone increased DNA synthesis and decreased creatine kinase activity, indicating stimulation of cell proliferation and inhibition of myogenesis, in undifferentiated myoblasts (1 day of culture). At longer culture intervals, when myoblasts elongate and fuse to form differentiated myotubes, 1,25(OH)2D3 promoted myogenesis, as indicated by an inhibition of DNA synthesis and an increase in specific muscle differentiation markers as creatine kinase activity and myosin expression. The role of protein kinase C (PKC) in mediating the effects of hormone and the likely PKC isoform involved were also investigated. Increased PKC activity was observed during 1,25(OH)2D3 stimulation of myoblast proliferation whereas inhibition of PKC activity accompanied the effects of the hormone on myoblast differentiation. The specific PKC inhibitor calphostin suppressed hormone potentiation of DNA synthesis in proliferating myoblasts. 1,25(OH)2D3-dependent changes in the expression of PKC isoforms α, β, δ, ε and ζ during myogenesis were investigated by Western blot analysis. The early stimulation of myoblast proliferation by the hormone mainly correlated to increased PKC α expression whereas decreased PKC α levels were observed during the subsequent activation of myoblast differentiation. These results support that 1,25(OH)2D3 has a function in embryonic muscle growth and maturation, and PKC α may participate in the signal transduction pathway which mediates the response to the hormone.

Registro:

Documento: Artículo
Título:Participation of protein kinase C α in 1,25-dihydroxy-vitamin D3 regulation of chick myoblast proliferation and differentiation
Autor:Capiati, D.A.; Téllez-Iñón, M.T.; Boland, R.L.
Filiación:Departamento de Biología, Bioquim. Y Farmacia, Univ. Nac. S., Bahia Blanca, Argentina
Inst. Invest. Ing. Genet. Y Biol. M., CONICET. Vuelta Obligado 2490, 1428, Buenos Aires, Argentina
Palabras clave:1,25(OH)2-Vitamin D3; Differentiation; Myoblasts; PKC isoenzymes; Protein kinase C; Skeletal muscle; calcitriol; protein kinase c; animal cell; article; cell differentiation; cell proliferation; DNA synthesis; enzyme activity; muscle development; nonhuman; priority journal; signal transduction; skeletal muscle; Animals; Calcitriol; Cell Differentiation; Cell Division; Cells, Cultured; Chick Embryo; Creatine Kinase; DNA; Enzyme Inhibitors; Isoenzymes; Muscle, Skeletal; Naphthalenes; Protein Kinase C; Time Factors
Año:1999
Volumen:153
Número:1-2
Página de inicio:39
Página de fin:45
DOI: http://dx.doi.org/10.1016/S0303-7207(99)00093-3
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:Calcitriol, 32222-06-3; calphostin complex; Creatine Kinase, EC 2.7.3.2; DNA, 9007-49-2; Enzyme Inhibitors; Isoenzymes; Naphthalenes; Protein Kinase C, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v153_n1-2_p39_Capiati

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Citas:

---------- APA ----------
Capiati, D.A., Téllez-Iñón, M.T. & Boland, R.L. (1999) . Participation of protein kinase C α in 1,25-dihydroxy-vitamin D3 regulation of chick myoblast proliferation and differentiation. Molecular and Cellular Endocrinology, 153(1-2), 39-45.
http://dx.doi.org/10.1016/S0303-7207(99)00093-3
---------- CHICAGO ----------
Capiati, D.A., Téllez-Iñón, M.T., Boland, R.L. "Participation of protein kinase C α in 1,25-dihydroxy-vitamin D3 regulation of chick myoblast proliferation and differentiation" . Molecular and Cellular Endocrinology 153, no. 1-2 (1999) : 39-45.
http://dx.doi.org/10.1016/S0303-7207(99)00093-3
---------- MLA ----------
Capiati, D.A., Téllez-Iñón, M.T., Boland, R.L. "Participation of protein kinase C α in 1,25-dihydroxy-vitamin D3 regulation of chick myoblast proliferation and differentiation" . Molecular and Cellular Endocrinology, vol. 153, no. 1-2, 1999, pp. 39-45.
http://dx.doi.org/10.1016/S0303-7207(99)00093-3
---------- VANCOUVER ----------
Capiati, D.A., Téllez-Iñón, M.T., Boland, R.L. Participation of protein kinase C α in 1,25-dihydroxy-vitamin D3 regulation of chick myoblast proliferation and differentiation. Mol. Cell. Endocrinol. 1999;153(1-2):39-45.
http://dx.doi.org/10.1016/S0303-7207(99)00093-3