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Garberi, J.C.; Kohane, A.C.; Cameo, M.S.; Blaquier, J.A. "Isolation and characterization of specific rat epididymal proteins" (1979) Molecular and Cellular Endocrinology. 13(1):73-82
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Abstract:

The partial purification and characterization of specific rat epididymal proteins (SEP) is reported. Starting from the cytosol fraction obtained from epididymal homogenates, protein C was purified 15-fold and proteins D-E were purified 19-fold. The molecular weight, determined by molecular sieving, of protein C was 22 400 while that of D-E was 37 000. These proteins stained as glycoproteins with periodic acid-Schiff reagent. The isoelectric point of protein D was 5.13 while that of protein E was 4.95. Protein C separated into 3 bands during isoelectric focussing. The major component focussed at 5.56 and the two minor components at pH 5.38 and 5.79. Using a specific antiserum we could confirm the organ specificity of SEP and their androgen-dependence. © 1979.

Registro:

Documento: Artículo
Título:Isolation and characterization of specific rat epididymal proteins
Autor:Garberi, J.C.; Kohane, A.C.; Cameo, M.S.; Blaquier, J.A.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:sperm maturation; glycoprotein; protein; animal; article; epididymis; immunodiffusion; isoelectric focusing; male; molecular weight; rat; Animal; Epididymis; Glycoproteins; Immunodiffusion; Isoelectric Focusing; Male; Molecular Weight; Proteins; Rats
Año:1979
Volumen:13
Número:1
Página de inicio:73
Página de fin:82
DOI: http://dx.doi.org/10.1016/0303-7207(79)90077-7
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:protein, 67254-75-5; Glycoproteins; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v13_n1_p73_Garberi

Referencias:

  • Alumot, Lensky, Schindler, (1971) J. Reprod. Fertil., 25, p. 349
  • Amman, Killian, Burton, (1973) J. Reprod. Fertil., 35, p. 321
  • Bedford, (1963) Nature (London), 200, p. 1178
  • Bedford, Development of the fertilizing ability of spermatozoa in the epididymis of the rabbit (1966) Journal of Experimental Zoology, 163, p. 319
  • Bedord, Effects of duct ligation on the fertilzing ability of spermatozoa from different regions of the rabbit epididymis (1967) Journal of Experimental Zoology, 166, p. 271
  • Blaquier, Cameo, Burgos, (1972) Endocrinology, 90, p. 839
  • Bonavida, (1971) Immunochemistry, 8, p. 829
  • Brandt, Acott, Johnson, Hoskins, (1977) Ann. Meeting Soc. Reprod. Abstract
  • Cameo, Blaquier, (1976) J. Endocrinol., 69, p. 47
  • Catsimpoolas, (1968) Anal. Biochem., 26, p. 480
  • Cummins, Effects of epididymal occlusion on sperm maturation in the hamster (1976) Journal of Experimental Zoology, 197, p. 187
  • Dyson, Orgebin-Crist, (1973) Endocrinology, 93, p. 391
  • Fournier, (1968) C.R. Soc. Biol., 160, p. 1087
  • Fournier-Delpech, Bayard, Boulard, (1973) C.R. Soc. Biol., 167, p. 1989
  • Grabar, Williams, (1953) Biochim. Biophys. Acta, 10, p. 193
  • Hamilton, (1975) Handbook of Physiology, 5, p. 259. , D.W. Hamilton, R.O. Greep, American Physiological Society, Washington D.C, Section 7
  • Himmelhoch, [26] Chromatography of proteins on ion-exchange adsorbents (1971) Methods Enzymology, 22, p. 273
  • Horan, Bedford, (1972) J. Reprod. Fertil., 30, p. 417
  • Jones, Glover, (1973) J. Reprod. Fertil, 34, p. 405
  • Koskimies, Kormano, PROTEINS IN FLUIDS FROM DIFFERENT SEGMENTS OF THE RAT EPIDIDYMIS (1975) Reproduction, 34, p. 345
  • Lavon, Volcani, Danon, (1971) J. Reprod. Fertil., 24, p. 219
  • Lea, Petrusz, French, Purification and Localization of Acidic Epididymal Glycoprotein (AEG): A Sperm Coating Protein Secreted by the Rat Epididymis (1978) International Journal of Andrology, p. 592. , Part 2
  • Lowry, Rosebrough, Farr, Randall, (1951) J. Biol. Chem., 193, p. 265
  • Lubicz-Nawrocki, (1974) J. Reprod. Fertil, 37, p. 251
  • Lubicz-Nawrocki, (1976) J. Exp. Zool., 197, p. 89
  • Olson, Hamilton, (1978) Biol. Reprod., 19, p. 26
  • Orgebin-Crist, MATURATION OF SPERMATOZOA IN THE RABBIT EPIDIDYMIS : FERTILIZING ABILITY AND EMBRYONIC MORTALITY IN DOES INSEMINATED WITH EPIDIDYMAL SPERMATOZOA (1967) Annales de Biologie Animale Biochimie Biophysique, 7, p. 373
  • Orgebin-Crist, Studies on the Function of the Epididymis (1969) Biology of Reproduction, p. 155
  • Orgebin-Crist, (1973) J. Exp. Zool., 185, p. 301
  • Orgebin-Crist, Jahad, (1978) Endocrinology, 103, p. 46
  • Orgebin-Crist, Danzo, Davies, Endocrine control of the development and maintenance of sperm fertilizing ability in the epididymis (1975) Handbook of Physiology, 5, p. 319. , D.W. Hamilton, R.O. Greep, American Physiological Society, Washington D.C, section 7
  • Ouchterlony, (1967) Handbook of Experimental Immunology, p. 655. , D.M. Weir, Blackwell, Oxford
  • Wong, Yeung, (1977) Endocrinology, 101, p. 1391
  • Zacharius, Zell, Morrison, Woodlock, (1969) Anal. Biochem., 30, p. 148

Citas:

---------- APA ----------
Garberi, J.C., Kohane, A.C., Cameo, M.S. & Blaquier, J.A. (1979) . Isolation and characterization of specific rat epididymal proteins. Molecular and Cellular Endocrinology, 13(1), 73-82.
http://dx.doi.org/10.1016/0303-7207(79)90077-7
---------- CHICAGO ----------
Garberi, J.C., Kohane, A.C., Cameo, M.S., Blaquier, J.A. "Isolation and characterization of specific rat epididymal proteins" . Molecular and Cellular Endocrinology 13, no. 1 (1979) : 73-82.
http://dx.doi.org/10.1016/0303-7207(79)90077-7
---------- MLA ----------
Garberi, J.C., Kohane, A.C., Cameo, M.S., Blaquier, J.A. "Isolation and characterization of specific rat epididymal proteins" . Molecular and Cellular Endocrinology, vol. 13, no. 1, 1979, pp. 73-82.
http://dx.doi.org/10.1016/0303-7207(79)90077-7
---------- VANCOUVER ----------
Garberi, J.C., Kohane, A.C., Cameo, M.S., Blaquier, J.A. Isolation and characterization of specific rat epididymal proteins. Mol. Cell. Endocrinol. 1979;13(1):73-82.
http://dx.doi.org/10.1016/0303-7207(79)90077-7