Abstract:
Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic β-(1,2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobility on SDS-polyacrylamide gels (apparent mol. mass of 320 kDa) than the β-(1,2)-glucan intermediate protein of Rhizobium meliloti. In vivo pulse-label experiments with growing cells of R. leguminosarum biovar trifolii RS800 using radioactive glucose showed that few species of cyclic β-(1,2)-glucans were synthesized and up to 30% were substituted with charged non-glycosidic residues, probably sn-1-phosphoglycerol. © 1995 Springer-Verlag.
Registro:
Documento: |
Artículo
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Título: | Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
Autor: | Castro, O.A.; Zorreguieta, A.; Semino, C.; Ielpi, L. |
Filiación: | Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and CONICET, Avenida Patricias Argentinas 435, Buenos Aires, 1405, Argentina
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Palabras clave: | Biovar phaseoli; Biovar trifolii; Biovar viciae; Cyclic β-(1,2)-glucan; Glucoprotein intermediate; Polysaccharide synthesis; Rhizobium leguminosarum biovars; beta glucan; glycerophosphate; glycoprotein; oligosaccharide; article; bacterial membrane; bacterial metabolism; carbohydrate synthesis; priority journal; Rhizobium leguminosarum; Rhizobium radiobacter; Rhizobium trifolii; Sinorhizobium meliloti; Agrobacterium tumefaciens; Bacteria (microorganisms); Rhizobium leguminosarum; Sinorhizobium meliloti |
Año: | 1995
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Volumen: | 163
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Número: | 6
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Página de inicio: | 454
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Página de fin: | 462
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DOI: |
http://dx.doi.org/10.1007/BF00272135 |
Título revista: | Archives of Microbiology
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Título revista abreviado: | Arch. Microbiol.
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ISSN: | 03028933
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CODEN: | AMICC
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03028933_v163_n6_p454_Castro |
Referencias:
- Altabe, S., Iñon de Ianino, N., de Mendoza, D., Ugalde, R., Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp (1990) J Bacteriol, 172, pp. 2563-2567
- Amemura, A., Synthesis of (1–2)-β-D-glucan by cell-free extracts of Agrobacterium radiobacter IFO 12665b1 and Rhizobium phaseoli AHU 1133 (1984) Agricultural and Biological Chemistry, 48, pp. 1809-1817
- Amemura, A., Properties and in vitro synthesis of cyclic (1–2)-β- d-glucan (1985) Mem Inst Sci Ind Res, Osaka Univ, 42, pp. 69-79
- Amemura, A., Hisamatsu, M., Mitani, H., Harada, T., Cyclic (1–2)-β- d-glucan and the oligosaccharide repeating-units of extracellular acidic polysaccharides produced by Rhizobium (1983) Carbohydr Res, 114, pp. 227-285
- Batley, M., Redmond, J.W., Djordjevic, S.P., Rolfe, B.G., Characterization of glycerophosphorylated cyclic β-1,2-glucans from a fast-growing Rhizobium species (1987) Biochim Biophys Acta, 901, pp. 119-126
- Bhagwat, A.A., Keister, D.L., Synthesis of β-glucans by Bradyrhizobium japonicum and Rhizobium fredii (1992) Can J Microbiol, 38, pp. 510-514
- Bhagwat, A.A., Tully, R.E., Keister, D.L., Isolation and characterization of and ndvB locus from Rhizobium fredii (1992) Mol Microbiol, 6, pp. 2159-2165
- Bossio, J.C., Iñon de Iannino, N., Dankert, M.A., In vitro synthesis of a lipid-linked acetylated and pyruvilated oligosaccharide in Rhizobium trifolii (1986) Biochem Biophys Res Commun, 134, pp. 205-211
- Bray, G.A., A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter (1960) Anal Biolchem, 1, pp. 279-285
- Breedveld, M.W, Zevenhuizen, L.P.T.M., Zehnder, A.J.B., Excessive excretion of cyclic β-(1,2)-glucan by Rhizobium trifolii TA-1 (1990) Appl Environ Microbiol, 56, pp. 2080-2086
- Breedveld, M.W., Zevenhuizen, L.P.T.M., Zehnder, A.J.B., Osmotically regulated trehalose accumulation and cyclic β-(1,2)-glucan excretion by Rhizobium leguminosarum biovar trifolii TA-1 (1991) Arch Microbiol, 156, pp. 501-506
- Breedveld, M.W., Zevenhuizen, L.P.T.M., Zehnder, A.J.B., Synthesis of cyclic β-(1,2)-glucans by Rhizobium leguminosarum biovar trifolii TA-1: factors influencing excretion (1992) J Bacteriol, 174, pp. 6336-6342
- Breedveld, M.W., Canter Cremers, H.C.J., Batley, M., Posthumus, M.A., Zevenhuizen, L.P.T.M., Wijffelman, C.A., Zehnder, A.J.B., Polysaccharide synthesis in relation to nodulation behavior of Rhizobium leguminosarum (1993) J Bacteriol, 175, pp. 750-757
- Brewin, N.J., Dejong, T.M., Phillips, D.A., Johnston, A.W.B., Cotransfer of determinants for hydrogenase activity and nodulation ability in Rhizobium leguminosarum (1980) Nature, 288, pp. 77-79
- Cangelosi, G.A., Martinetti, G., Leigh, J.A., Lee, C.C., Theines, C., Nester, E.W., Role of Agrobacteium tumefaciens ChvA protein in export of β-1,2-glucan (1989) J Bacteriol, 171, pp. 1609-1615
- Chamberlain, J.P., Fluorographic detection of radioactivity in polyacrilamide gels with the water-soluble fluor, sodium salicylate (1979) Anal Biochem, 98, pp. 132-135
- Ditta, G., Stanfield, S., Corbin, D., Helinski, D.R., Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti (1980) Proc Natl Acad Sci USA, 77, pp. 7347-7351
- Douglas, C.J., Staneloni, R.J., Rubin, R.A., Nester, E.W., Identification and genetic analysis of an Agrabacterium tumefaciens chromosomal virulence region (1985) J Bacteriol, 161, pp. 850-860
- Dylan, T., Ielpi, L., Stanfield, S., Kashyap, L., Douglas, C., Yanofsky, M., Nester, E.W., Ditta, G., Rhizobium meliloti genes required for nodule development are related to chromosomal virulence genes in Agrobacterium tumefaciens (1986) Proc Natl Acad Sci USA, 83, pp. 4403-4407
- Dylan, T., Helinski, D.R., Ditta, G.S., Hypoosmotic adaptation in Rhizobium meliloti requires β-(1–2)-glucan (1990) J Bacteriol, 172, pp. 1400-1408
- Geiger, O., Weissborn, A.C., Kennedy, E.P., Biosynthesis and excretion of cyclic glucans by Rhizobium meliloti 1021 (1991) J Bacteriol, 173, pp. 3021-3024
- Geremía, R.A., Cavaignac, S., Zorreguieta, A., Toro, N., Olivares, J., Ugalde, R.A., A Rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharides, but fails to form β-(1-2)glucan (1987) J Bacteriol, 169, pp. 880-884
- Ielpi, L., Dylan, T., Ditta, G.S., Helinski, D.R., Stanfield, S.W., The ndvB locus of Rhizobium meliloti encodes a 319-kDa protein involved in the production of β-(1–2)-glucan (1990) J Biol Chem, 265, pp. 2843-2851
- Iñón de Iannino, N., Ugalde, R.A., Biochemical characterization of avirulent Agrobacterium tumefaciens chvA mutants: synthesis and excretion of β-(1,2)-glucan (1989) J Bacteriol, 171, pp. 2842-2849
- Koepsell, H.J., Sharpe, E.S., Microdetermination of pyruvic and α-ketoglutaric acids (1952) Arch Biochem Biophys, 38, pp. 443-449
- Lamb, J.W., Hombrecher, G., Johnston, A.W.B., Plasmid-determined nodulation and nitrogen-fixation abilities in Rhizobium phaseoli (1982) Mol Gen Genet, 186, pp. 449-452
- Lepek, V., Navarro de Navarro, Y., Ugalde, R.A., Synthesis of β(1–2)glucan in Rhizobium loti. Expression of Agrobacterium tumefaciens chvB virulence region (1990) Arch Microbiol, 155, pp. 35-41
- Megías, M., Caviedes, M.A., Palomares, A.J., Perez-Silva, J., Use of plasmid R68.45 for constructing a circular linkage map of the Rhizobium trifolii chromosome (1982) J Bacteriol, 149, pp. 59-64
- Miller, K.J., Kennedy, E.P., Reinhold, V.N., Osmotic adaption by gram-negative bacteria: possible role of periplasmic oligosaccharides (1986) Science, 231, pp. 48-51
- Miller, K.J., Reinhold, V.N., Weissborn, A.C., Kennedy, E.P., Cyclic glucans produced by Agrobacterurium tumefaciens are substituted with sn-1-phosphoglycerol residues (1987) Biochim Biophys Acta, 901, pp. 112-118
- Miller, K.J., Gore, R.S., Benesi, A.J., Phosphoglycerol substituents present on the cyclic β-1,2-glucans of Rhizobium meliloti 1021 are derived from phosphatidylglycerol (1988) J Bacteriol, 170, pp. 4569-4575
- Osborn, M.J., Munson, R., Separaration of inner (cytoplasmic) and outer membranes of gram-negative bacteria (1984) Methods Enzymol, 31 A, pp. 642-653
- Puvanesarajah, V., Schell, F.M., Stacey, G., Douglas, C.J., Nester, E.W., (1985) Role for 2-linked-β-d-glucan in the virulence of Agrobacterium, 164, pp. 102-106
- Schneider, J.E., Reinhold, V., Rumley, M.K., Kennedy, E.P., Structural studies of the membrane-derived oligosaccharides of Escherichia coli (1979) J Biol Chem, 254, pp. 10135-10138
- Stanfield, S.W., Ielpi, L., O'Brochta, D., Helinski, D.R., Ditta, G.S., The ndvA gene product of Rhizobium meliloti is required for β-(1,2)-glucan production and has homology to the ATP-binding export protein HlyB (1988) J Bacteriol, 170, pp. 3523-3530
- Studier, F.W., Analysis of bacteriophage T7 early RNAs and proteins on slab gels (1973) J Mol Biol, 79, pp. 237-248
- Therisod, H., Kennedy, E.P., The function of acyl carrier protein in the synthesis of membrane-derived oligosaccharides does not require its phosphopantetheine prosthetic group (1987) Proc Natl Acad Sci USA, 84, pp. 8235-8238
- Therisod, H., Weissborn, A.C., Kennedy, E.P., An essential function for acyl carrier protein in the biosynthesis of membrane-derived oligosaccharides of Escherichia coli (1986) Proc Natl Acad Sci USA, 83, pp. 7236-7240
- Virji, M., Saunders, J.R., Si, G., Makepeace, K., Maskell, D., Ferguson, D.J.P., Pilus-facilitated adherence of Neisseria meningitidis to human epithelial and endothelial cells: modulation of adherence phenotype occurs concurrently with changes in primary amino acid sequence and the glycosylation status of pilin (1993) Mol Microbiol, 10, pp. 1013-1028
- Weissborn, A.C., Kennedy, E.P., Biosynthesis of membrane-derived oligosaccharides (1984) J Biol Chem, 259, pp. 12644-12651
- Weissborn, A.C., Rumley, M.K., Kennedy, E.P., Biosynthesis of membrane-derived oligosaccharides. Membrane-bound glucosyltransferase system from Escherichia coli requires polyprenyl phosphate (1991) J Biol Chem, 266, pp. 8062-8067
- Willia, A.M., Enns, C.A., A mutated transferrin receptor lacking asparagine-linked glycosylation sites shows reduced functionality and an association with binding immunoglobulin protein (1991) J Biol Chem, 266, pp. 17648-17654
- Williaon, G., Damani, K., Devenney, P., Faulds, C.B., Morris, V.J., Stevens, B.J.H., Mechanism of action of cyclic β-1,2-glucan synthetase from Agrobacterium tumefaciens: competition between cyclization and elongation reactions (1992) J Bacteriol, 174, pp. 7941-7947
- Zevenhuizen, L.P.T.M., Van Veldhuizen, A., Fokkens, R.H., Reexamination of cellular cyclic β-1,2-glucans of Rhizobiaceae: distribution of ring sizes and degrees of glycerol-1-phosphate substitution (1990) Antoine Van Leeuwenhoek, 57, pp. 173-178
- Zorreguieta, A., Ugalde, R.A., Formation in Rhizobium and Agrobacterium spp. of a 235-kilodalton protein intermediate in β-D(1–2)-glucan synthesis (1986) J Bacteriol, 167, pp. 947-951
- Zorreguieta, A., Tolmasky, M.E., Staneloni, R.J., The enzymatic synthesis of β-1–2-glucans (1985) Arch Biochem Biophys, 238, pp. 368-372
- Zorreguieta, A., Ugalde, R.A., Leloir, L.F., An intermediate in cyclic β-1–2-glucan biosynthesis (1985) Biochem Biophys Res Commun, 126, pp. 352-357
- Zorreguieta, A., Geremía, R., Cangelosi, G.A., Nester, E.W., Ugalde, R.A., Identification of the product of an Agrobacterium tumefaciens chromosomal virulence gene (1988) Mol Plant Microbe Interact, 1, pp. 121-127
- Zorreguieta, A., Cavaignac, S., Geremía, R.A., Ugalde, R.A., Osmotic regulation of β(1–2)glucan synthesis in members of the family Rhizobiaceae (1990) J Bacteriol, 172, pp. 4701-4704
Citas:
---------- APA ----------
Castro, O.A., Zorreguieta, A., Semino, C. & Ielpi, L.
(1995)
. Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii. Archives of Microbiology, 163(6), 454-462.
http://dx.doi.org/10.1007/BF00272135---------- CHICAGO ----------
Castro, O.A., Zorreguieta, A., Semino, C., Ielpi, L.
"Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii"
. Archives of Microbiology 163, no. 6
(1995) : 454-462.
http://dx.doi.org/10.1007/BF00272135---------- MLA ----------
Castro, O.A., Zorreguieta, A., Semino, C., Ielpi, L.
"Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii"
. Archives of Microbiology, vol. 163, no. 6, 1995, pp. 454-462.
http://dx.doi.org/10.1007/BF00272135---------- VANCOUVER ----------
Castro, O.A., Zorreguieta, A., Semino, C., Ielpi, L. Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii. Arch. Microbiol. 1995;163(6):454-462.
http://dx.doi.org/10.1007/BF00272135