Abstract:
The metabolism of trehalose in wild type cells of Escherichia coli and Salmonella typhimurium has been investigated. Intact cells of Escherichia coli (grown on trehalose) accumulated [14C]-trehalose as [14C]-trehalose 6-phosphate. Toluene-treated cells catalyzed the synthesis of the [14C]-sugar phosphate from [14C]-trehalose and phosphoenolpyruvate; ATP did not serve as phosphoryl donor. Trehalose 6-phosphate could subsequently be hydrolyzed by trehalose 6-phosphate hydrolase, an enzyme which catalyzes the hydrolysis of the disaccharide phosphate into glucose and glucose 6-phosphate. Both Escherichia coli and Salmonella typhimurium induced this enzyme when they grew on trehalose. These findings suggest that trehalose is transported in these bacteria by an inducible phosphoenolpyruvate:trehalose phosphotransferase system. The presence of a constitutive trehalase was also detected. © 1984 Springer-Verlag.
Registro:
Documento: |
Artículo
|
Título: | Transport and metabolism of trehalose in Escherichia coli and Salmonella typhimurium |
Autor: | Maréchal, L.R. |
Filiación: | Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Obligado 2490, Buenos Aires, RA-1428, Argentina
|
Palabras clave: | Escherichia coli; Phosphoenolpyruvate:trehalose phosphotransferase system; Salmonella typhimurium; Transport; Trehalase; Trehalose; Trehalose 6-phosphate hydrolase; radioisotope; trehalose; cell membrane transport; escherichia coli; metabolism; nonhuman; salmonella typhimurium; trehalose c 14; Biological Transport; Disaccharidases; Disaccharides; Escherichia coli; Glucose; Glycerol; Salmonella typhimurium; Support, Non-U.S. Gov't; Trehalase; Trehalose |
Año: | 1984
|
Volumen: | 137
|
Número: | 1
|
Página de inicio: | 70
|
Página de fin: | 73
|
DOI: |
http://dx.doi.org/10.1007/BF00425810 |
Título revista: | Archives of Microbiology
|
Título revista abreviado: | Arch. Microbiol.
|
ISSN: | 03028933
|
CODEN: | AMICC
|
CAS: | trehalose, 99-20-7; alpha, alpha-phosphotrehalase, EC 3.2.1.93; Disaccharidases, EC 3.2.1.-; Disaccharides; Glucose, 50-99-7; Glycerol, 56-81-5; Trehalase, EC 3.2.1.28; Trehalose, 99-20-7
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03028933_v137_n1_p70_Marechal |
Referencias:
- Becerra de Lares, L., Ratouchniak, J., Casse, F., Chromosomal location of gene governing the trehalose utilization in Escherichia coli K-12 (1977) Molec Gen Genet, 152, pp. 105-108
- Bhumiratana, A., Anderson, R.L., Costilow, R.N., Trehalose metabolism by Bacillus popilliae (1974) J Bacteriol, 119, pp. 484-493
- Hengstenberg, W., Egan, J.B., Morse, M.L., Carbohydrate transport in Staphylococcus aureus. The nature of the derivatives accumulated (1968) J Biol Chem, 243, pp. 1881-1885
- Lowry, O.H., Rosebrough, H.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
- Miller, J.H., (1972) Experiments in molecular genetics, pp. 431-435. , Cold Spring Harbor Laboratory, Boca Raton
- Nelson, N., A photometric determination of the Somogyi method for the determination of glucose (1944) J Biol Chem, 153, pp. 375-380
- Postma, P.W., Roseman, S., The bacterial phosphoenolpyruvate: sugar phosphotransferase system (1976) Biochim Biophys Acta, 457, pp. 213-257
- Saint Pierre, M.L., Isolation and mapping of Salmonella typhimurium mutants defective in the utilization of trehalose (1968) J Bacteriol, 95, pp. 1185-1186
- Simoni, R., Hays, J., Nakazawa, T., Roseman, S., Sugar transport. VI. Phosphoryl transfer in the lactose phosphotransferase system of Staphylococcus aureus (1973) J Biol Chem, 248, pp. 957-965
- Somogyi, M., A new reagent for the determination of sugars (1945) J Biol Chem, 160, pp. 61-68
- Thompson, J., Chassy, B.M., Uptake and metabolism of sucrose by Streptococcus lactis (1981) J Bacteriol, 147, pp. 543-551
Citas:
---------- APA ----------
(1984)
. Transport and metabolism of trehalose in Escherichia coli and Salmonella typhimurium. Archives of Microbiology, 137(1), 70-73.
http://dx.doi.org/10.1007/BF00425810---------- CHICAGO ----------
Maréchal, L.R.
"Transport and metabolism of trehalose in Escherichia coli and Salmonella typhimurium"
. Archives of Microbiology 137, no. 1
(1984) : 70-73.
http://dx.doi.org/10.1007/BF00425810---------- MLA ----------
Maréchal, L.R.
"Transport and metabolism of trehalose in Escherichia coli and Salmonella typhimurium"
. Archives of Microbiology, vol. 137, no. 1, 1984, pp. 70-73.
http://dx.doi.org/10.1007/BF00425810---------- VANCOUVER ----------
Maréchal, L.R. Transport and metabolism of trehalose in Escherichia coli and Salmonella typhimurium. Arch. Microbiol. 1984;137(1):70-73.
http://dx.doi.org/10.1007/BF00425810