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Abstract:

Rhodopseudomonas viridis was grown in the presence and in the absence of yeast extract. The cells grown under this latter condition present a ten fold diminished bacteriochlorophyll (bchl) content. This decrease was paralleled by a similar increase in the ratio lipid phosphorous/bchl, whereas the ornithine lipid/bchl ratio remains constant. Some quantitative differences in the fatty acid composition are also reported. The protein composition of both membranes was also studied, only indicating quantitative differences. An active reaction center preparation was obtained from both types of cells. When isolated from cells grown in the presence of yeast extract, this reaction center preparation shows the presence of proteins a, b, c and d. Further treatment of this active reaction center results, in cells grown under either condition, in the isolation of green (oxidized behl) and brown (inactive reaction center) bchl containing fractions. The protein composition and absorption spectrum of the inactive reaction centers obtained from both types of cells were identical (proteins a, c and d). On the other hand the green complexes differ in their protein composition as well as in their absorption spectrum. © 1974 Springer-Verlag.

Registro:

Documento: Artículo
Título:Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract
Autor:Pucheu, N.L.; Kerber, N.L.; García, A.F.
Filiación:Centro de Investigaciones Microbiológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Lipid and Protein Composition; Photosynthetic Membrane; Reaction Center; bacteriochlorophyll; lipid; ornithine; protein; bacterial cell wall; in vitro study; microorganism; rhodopseudomonas viridis; theoretical study; Bacterial Proteins; Cell Fractionation; Cell Membrane; Chlorophyll; Chromatography, Thin Layer; Comparative Study; Culture Media; Electrophoresis; Fatty Acids; Lipids; Ornithine; Phosphorus; Rhodopseudomonas; Saccharomyces cerevisiae
Año:1974
Volumen:101
Número:1
Página de inicio:259
Página de fin:272
DOI: http://dx.doi.org/10.1007/BF00455943
Título revista:Archives of Microbiology
Título revista abreviado:Arch. Microbiol.
ISSN:03028933
CODEN:AMICC
CAS:lipid, 66455-18-3; ornithine, 70-26-8, 7006-33-9; protein, 67254-75-5; Bacterial Proteins; Chlorophyll, 1406-65-1; Culture Media; Fatty Acids; Lipids; Ornithine, 7006-33-9; Phosphorus, 7723-14-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03028933_v101_n1_p259_Pucheu

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Citas:

---------- APA ----------
Pucheu, N.L., Kerber, N.L. & García, A.F. (1974) . Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract. Archives of Microbiology, 101(1), 259-272.
http://dx.doi.org/10.1007/BF00455943
---------- CHICAGO ----------
Pucheu, N.L., Kerber, N.L., García, A.F. "Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract" . Archives of Microbiology 101, no. 1 (1974) : 259-272.
http://dx.doi.org/10.1007/BF00455943
---------- MLA ----------
Pucheu, N.L., Kerber, N.L., García, A.F. "Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract" . Archives of Microbiology, vol. 101, no. 1, 1974, pp. 259-272.
http://dx.doi.org/10.1007/BF00455943
---------- VANCOUVER ----------
Pucheu, N.L., Kerber, N.L., García, A.F. Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract. Arch. Microbiol. 1974;101(1):259-272.
http://dx.doi.org/10.1007/BF00455943