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Abstract:

The vertebrate olfactory system has fascinated neurobiologists over the last six decades because of its ability to replace its neurons and synaptic connections continuously throughout adult life, under both physiological and pathological conditions. Among the factors that are proposed to be involved in this regenerative potential, brain-derived neurotrophic factor (BDNF) is a candidate for having an important role in the neuronal turnover in the olfactory epithelium (OE) because of its well-documented neurogenic and trophic effects throughout the nervous system. The aim of the present study was to generate a suitable model to study the participation of BDNF in the recovery of the OE after injury in vivo. We developed an experimental design in which the OE of Rhinella arenarum tadpoles could be easily and selectively damaged by immersing the animals in ZnSO4 solutions of various concentrations for differing time periods. Image analysis of histological sections showed that different combinations of each of these conditions produced statistically different degrees of injury to the olfactory tissue. We also observed that the morphology of the OE was restored within a few days of recovery after ZnSO 4 treatment. Immunohistochemical analysis of BDNF was performed with an antiserum whose specificity was confirmed by Western blotting, and which showed drastic changes in the abundance and distribution pattern of this neurotrophin in the damaged olfactory system. Our results thus suggest that BDNF is involved in the regeneration of the OE of amphibian larvae, and that our approach is suitable for further investigations of this topic. © Springer-Verlag 2009.

Registro:

Documento: Artículo
Título:Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium
Autor:Yovanovich, C.A.M.; Jungblut, L.D.; Heer, T.; Pozzi, A.G.; Paz, D.A.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Universidad de Buenos Aires and IFIBYNE - CONICET, Ciudad Universitaria Pabellón II 4To Piso, (C1428EHA) Buenos Aires, Argentina
Palabras clave:Neurotrophins; Olfactory neuron; Regeneration; Rhinella arenarum (bufo arenarum) (anura); brain derived neurotrophic factor; zinc sulfate; Amphibia; animal; animal embryo; animal model; article; biological model; cell proliferation; drug effect; evaluation; growth, development and aging; injury; larva; metabolism; nerve cell; nerve regeneration; olfactory epithelium; olfactory nerve; physiology; prenatal development; randomization; Amphibians; Animals; Brain-Derived Neurotrophic Factor; Cell Proliferation; Embryo, Nonmammalian; Larva; Models, Animal; Models, Biological; Nerve Regeneration; Neurons; Olfactory Mucosa; Olfactory Nerve; Random Allocation; Zinc Sulfate
Año:2009
Volumen:336
Número:1
Página de inicio:1
Página de fin:9
DOI: http://dx.doi.org/10.1007/s00441-009-0752-7
Título revista:Cell and Tissue Research
Título revista abreviado:Cell Tissue Res.
ISSN:0302766X
CODEN:CTSRC
CAS:brain derived neurotrophic factor, 218441-99-7; zinc sulfate, 7733-02-0; Brain-Derived Neurotrophic Factor; Zinc Sulfate, 7733-02-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v336_n1_p1_Yovanovich

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Citas:

---------- APA ----------
Yovanovich, C.A.M., Jungblut, L.D., Heer, T., Pozzi, A.G. & Paz, D.A. (2009) . Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium. Cell and Tissue Research, 336(1), 1-9.
http://dx.doi.org/10.1007/s00441-009-0752-7
---------- CHICAGO ----------
Yovanovich, C.A.M., Jungblut, L.D., Heer, T., Pozzi, A.G., Paz, D.A. "Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium" . Cell and Tissue Research 336, no. 1 (2009) : 1-9.
http://dx.doi.org/10.1007/s00441-009-0752-7
---------- MLA ----------
Yovanovich, C.A.M., Jungblut, L.D., Heer, T., Pozzi, A.G., Paz, D.A. "Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium" . Cell and Tissue Research, vol. 336, no. 1, 2009, pp. 1-9.
http://dx.doi.org/10.1007/s00441-009-0752-7
---------- VANCOUVER ----------
Yovanovich, C.A.M., Jungblut, L.D., Heer, T., Pozzi, A.G., Paz, D.A. Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium. Cell Tissue Res. 2009;336(1):1-9.
http://dx.doi.org/10.1007/s00441-009-0752-7