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Abstract:

The presence and distribution of gonadotropin-releasing hormone (GnRH) in sexually mature specimens of Bufo arenarum was studied by reverse phase/high performance liquid chromatography (RP-HPLC) combined with radioimmunoassay and immunocytochemistry. The analysis of brain extracts with RP-HPLC followed by radioimmunoassay with PBL45 antiserum showed the presence of only one peak with immunoreactivity for GnRH (ir-GnRH) having the chromatographic and immunological characteristics of mammalian GnRH (mGnRH). This peak was further analyzed with two mGnRH-specific antisera, EL-15 and m1076, yielding serial dilution displacement curves parallel to those obtained with the mGnRH synthetic standard. Immunocytochemical results with the monoclonal antibody LRH13 showed the presence of a terminal nerve-septo-preoptic system with neurons and fibers distributed from the olfactory bulb, septal area, and anterior preoptic area toward the hypothalamus and hypophyseal neural lobe. The main group of ir-GnRH fibers and neurons was identified in the anterior preoptic area. These neurons appear to be the origin of fibers that, after surrounding the preoptic recess, border the dorsal surface of the optic chiasma, extend through the infundibulum, traverse the external layer of the median eminence, and end in the pars nervosa.

Registro:

Documento: Artículo
Título:Identification and neuroanatomical distribution of immunoreactivity for mammalian gonadotropin-releasing hormone (mGnRH) in the brain and neural hypophyseal lobe of the toad Bufo arenarum
Autor:Miranda, L.A.; Paz, D.A.; Affanni, J.M.; Somoza, G.M.
Filiación:Instituto de Neurociencia, Depto. de Ciencias Biológicas, Pabelión II, (1428) Buenos Aires, Argentina
Palabras clave:Brain; Bufo arenarum (Anura); Gonadotropin-releasing hormone; Hypophyseal neural lobe (pars nervosa); Reproduction; gonadorelin; animal tissue; article; female; immunocytochemistry; infundibulum; male; median eminence; neuroanatomy; neurohypophysis; nonhuman; optic chiasm; preoptic area; priority journal; radioimmunoassay; reversed phase high performance liquid chromatography; toad; Animals; Brain; Brain Chemistry; Bufo arenarum; Chromatography, High Pressure Liquid; Female; Gonadotropin-Releasing Hormone; Immunohistochemistry; Male; Pituitary Gland, Posterior; Radioimmunoassay; Animalia; Anura; Bufo arenarum; Mammalia
Año:1998
Volumen:293
Número:3
Página de inicio:419
Página de fin:425
DOI: http://dx.doi.org/10.1007/s004410051133
Título revista:Cell and Tissue Research
Título revista abreviado:Cell Tissue Res.
ISSN:0302766X
CODEN:CTSRC
CAS:Gonadotropin-Releasing Hormone, 33515-09-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v293_n3_p419_Miranda

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Citas:

---------- APA ----------
Miranda, L.A., Paz, D.A., Affanni, J.M. & Somoza, G.M. (1998) . Identification and neuroanatomical distribution of immunoreactivity for mammalian gonadotropin-releasing hormone (mGnRH) in the brain and neural hypophyseal lobe of the toad Bufo arenarum. Cell and Tissue Research, 293(3), 419-425.
http://dx.doi.org/10.1007/s004410051133
---------- CHICAGO ----------
Miranda, L.A., Paz, D.A., Affanni, J.M., Somoza, G.M. "Identification and neuroanatomical distribution of immunoreactivity for mammalian gonadotropin-releasing hormone (mGnRH) in the brain and neural hypophyseal lobe of the toad Bufo arenarum" . Cell and Tissue Research 293, no. 3 (1998) : 419-425.
http://dx.doi.org/10.1007/s004410051133
---------- MLA ----------
Miranda, L.A., Paz, D.A., Affanni, J.M., Somoza, G.M. "Identification and neuroanatomical distribution of immunoreactivity for mammalian gonadotropin-releasing hormone (mGnRH) in the brain and neural hypophyseal lobe of the toad Bufo arenarum" . Cell and Tissue Research, vol. 293, no. 3, 1998, pp. 419-425.
http://dx.doi.org/10.1007/s004410051133
---------- VANCOUVER ----------
Miranda, L.A., Paz, D.A., Affanni, J.M., Somoza, G.M. Identification and neuroanatomical distribution of immunoreactivity for mammalian gonadotropin-releasing hormone (mGnRH) in the brain and neural hypophyseal lobe of the toad Bufo arenarum. Cell Tissue Res. 1998;293(3):419-425.
http://dx.doi.org/10.1007/s004410051133