Protein synthesis was studied in polyamine-auxotrophic mutants of E. coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of peptides synthesized in vivo suggests a more extensive misreading by putrescine-depleted bacteria. Cells grown without polyamine show a markedly decreased response to the inhibitory effect and the misreading inducing action of streptomycin in vivo and in vitro. The polyamine-starved bacteria are also less sensitive to other aminoglycoside antibiotics such as neomycin, kanamycin and kasugamycin.
| Documento: | Artículo |
| Título: | Polyamines and antibiotic effects on translation |
| Autor: | Goldemberg, S.H.; Algranati, I.D. |
| Filiación: | Inst. Invest. Bioquim., Fund. Campomar, 1428 Buenos Aires, Argentina |
| Palabras clave: | kanamycin; kasugamycin; neomycin; polyamine; putrescine; radioisotope; spermidine; spermine; streptomycin; tetracycline; cell malformation; dihydrostreptomycin h 3; dose response; drug comparison; drug response; escherichia coli; in vitro study; leucine c 14; methionine s 35; phenylalanine c 14; protein synthesis; Aminoglycosides; Antibiotics; Bacterial Proteins; Cell-Free System; Escherichia coli; Polyamines; Putrescine; Ribosomes; RNA, Transfer, Amino Acyl; Streptomycin; Support, Non-U.S. Gov't; Translation, Genetic |
| Año: | 1981 |
| Volumen: | 59 |
| Número: | 5-6 |
| Página de inicio: | 360 |
| Página de fin: | 367 |
| Título revista: | Medical Biology |
| Título revista abreviado: | MED. BIOL. |
| ISSN: | 03022137 |
| CODEN: | MDBYA |
| CAS: | kanamycin, 11025-66-4, 61230-38-4, 8063-07-8; kasugamycin, 6980-18-3; neomycin, 11004-65-2, 1404-04-2, 1405-10-3, 8026-22-0; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; spermine, 306-67-2, 71-44-3; streptomycin, 57-92-1; tetracycline, 23843-90-5, 60-54-8, 64-75-5; Aminoglycosides; Antibiotics; Bacterial Proteins; Polyamines; Putrescine, 110-60-1; RNA, Transfer, Amino Acyl; Streptomycin, 57-92-1; tRNA, formylmethionine- |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03022137_v59_n5-6_p360_Goldemberg |
