Abstract:
A total of 120 freshly harvested wheat samples from the 2004 season in nine locations from Northern Buenos Aires Province, Argentina, were analysed for trichothecene natural occurrence and associated mycoflora, and for determining the influence of commonly used fungicide field treatment and the cultivar type on trichothecene contamination. The trichothecenes T-2 tetraol, T-2 triol, HT-2 and T-2 toxin (HT-2, T-2), diacetoxyscirpenol (DAS), nivalenol (NIV), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON) and 15- acetyldeoxynivalenol (15-ADON) were analysed by gas chromatography and electron capture detection. Detection limits ranged from 4 to 20 μg/kg. The isolation frequencies of species were calculated. Alternaria alternata, Fusarium graminearum, Fusarium poae and Fusarium semitectum were the predominant fungal species identified as endogenous mycoflora. The type of cultivar and the fungicide field treatment did not affect significantly the trichothecene contamination. The trichothecenes type A detected were HT-2 and T-2 triol toxins and the type B were DON, NIV and 3-ADON. Based on 120 samples the incidences were 21.7% for 3-ADON, 22.5% for HT-2, 27.5% for T-2 triol and 85% for DON. NIV was confirmed in one sample. Mean levels of trichothecene positive samples were between 7 and 2788 μg/kg. © 2008 Springer Science+Business Media B.V.
Registro:
Documento: |
Artículo
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Título: | Trichothecenes and mycoflora in wheat harvested in nine locations in Buenos Aires Province, Argentina |
Autor: | González, H.H.L.; Moltó, G.A.; Pacin, A.; Resnik, S.L.; Zelaya, M.J.; Masana, M.; Martínez, E.J. |
Filiación: | Consejo Nacional de Investigaciones Científicas Y Técnicas, Buenos Aires, Argentina Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Buenos Aires, Argentina Departamento de Industrias, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, Buenos Aires C1428EHA, Argentina Departamento de Química Orgánica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Facultad de Bromatología, Universidad Nacional de Entre Ríos, Gualeguaychú, Argentina Fundación de Investigaciones Científicas Teresa Benedicta de la Cruz, Luján, Argentina Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Buenos Aires, Argentina Universidad Nacional de Luján, Lujan, Argentina Instituto Tecnología de Alimentos, INTA Castelar, Castelar, Argentina Instituto de Cálculo, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
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Palabras clave: | Alternaria; Deoxynivalenol; Fusarium; Mycoflora; Trichothecenes; Wheat; 15 acetylvomitoxin; 15-acetyldeoxynivalenol; 3 acetyldeoxynivalenol; 3-acetyldeoxynivalenol; diacetoxyscirpenol; nivalenol; trichothecene derivative; vomitoxin; Alternaria; Argentina; article; chemistry; classification; fungus; Fusarium; gas chromatography; isolation and purification; metabolism; microbiology; species difference; wheat; Alternaria; Argentina; Chromatography, Gas; Fungi; Fusarium; Species Specificity; Trichothecenes; Triticum; Alternaria; Alternaria alternata; Fusarium; Fusarium incarnatum; Fusarium poae; Gibberella zeae; Triticum aestivum |
Año: | 2008
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Volumen: | 165
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Número: | 2
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Página de inicio: | 105
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Página de fin: | 114
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DOI: |
http://dx.doi.org/10.1007/s11046-007-9084-x |
Título revista: | Mycopathologia
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Título revista abreviado: | Mycopathologia
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ISSN: | 0301486X
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CODEN: | MYCPA
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CAS: | 3 acetyldeoxynivalenol, 50722-38-8; diacetoxyscirpenol, 2270-40-8; nivalenol, 23282-20-4; vomitoxin, 51481-10-8; 15-acetyldeoxynivalenol, 88337-96-6; 3-acetyldeoxynivalenol, 50722-38-8; deoxynivalenol, 51481-10-8; diacetoxyscirpenol, 2270-40-8; nivalenol, 23282-20-4; Trichothecenes
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0301486X_v165_n2_p105_Gonzalez |
Referencias:
- Marasas, W.F.O., Smith, J.C., Henderson, R.S., Toxigenic Fusaria (1991) Animal Food, pp. 119-39. , CRC Press Inc Boca Raton
- Walker, S.L., Leath, S., Hagler, W.M., Murphy, J., Variation among isolates of Fusarium graminearum associated with Fusarium Head Blight in North Carolina (2001) Plant Dis, 85, pp. 404-10
- Bottalico, A., Perrone, G., Toxigenic Fusarium species and mycotoxins associated with head blight in small grains cereals in Europe (2002) Eur J Plant Pathol, 108, pp. 611-24
- Gallo, A., Bourdignon, F., Pacin, A., Barbieri, T., Resnik, S.L., Evaluation of food intake by means of 24 hour dietary recall in a town of the province of Buenos Aires, Argentina (1992) Ecol Food Nutr, 28, pp. 299-317
- Pacin, A., Martínez, E.J., Portela, M.L., Neira, M.S., Consumo de alimentos e ingesta de algunos nutrientes en la población de la Universidad Nacional de Luján, Argentina (1999) Archivos Latinoamericanos de Nutrición, 49, pp. 31-9
- Quiroga, N., Resnik, S.L., Pacin, A., Martínez, E.J., Pagano, E., Riccobene, I., Neira, S., Natural occurrence of trichothecenes and zearalenone in Argentine wheat (1995) Food Control, 6, pp. 201-4
- González, H.H.L., Pacin, A., Resnik, S.L., Martínez, E.J., Deoxynivalenol and contaminant mycoflora in freshly harvested Argentinean wheat in 1993 (1996) Mycopathologia, 135, pp. 129-34
- González, H.H.L., Martínez, E.J., Pacin, A., Resnik, S.L., Relationship between Fusarium graminearum and Alternaria alternata contamination and deoxynivalenol occurrence on Argentinean durum wheat (1999) Mycopathologia, 144, pp. 97-102
- Pacin, A., Resnik, S.L., Neira, S.M., Moltó, G., Martínez, E.J., Natural occurrence of deoxynivalenol in wheat, wheat flour and bakery products in Argentina (1997) Food Addit Contam, 14, pp. 327-31
- Dalcero, A., Torres, A., Etcheverry, M., Chulze, S., Varsavsky, E., Occurrence of deoxynivalenol and Fusarium graminearum in Argentinian wheat (1997) Food Addit Contam, 14, pp. 11-4
- Lori, G.A., Sisterna, M.N., Haidukowski, M., Rizzo, I., Fusarium graminearum and deoxynivalenol contamination in the durum wheat area of Argentina (2003) Microbiol Res, 158, pp. 29-35
- Faifer, G.C., Sala, M., Godoy, H.M., Patterns of mycotoxin production by Fusarium graminearum isolated from Argentine wheat (1990) Mycopathologia, 109, pp. 165-70
- Terminiello, L.A., Fernández Pinto, V.E., (2004) Estudio de Los Quimiotipos de Fusarium en Trigo Argentino, , Congreso Internacional de Ciencia y Tecnología de los Alimentos Córdoba
- Lori, G.A., Carranza, M.R., Violante, A., Rizzo, I., Alippi, H.E., Fusarium spp en trigo, capacidad toxicogénica y quimiotaxonomía de las cepas aisladas en la Argentina (1992) Agronomie, 12, pp. 459-67
- Alvarez, C., Somma, S., Stea, G., Cabral, D., Mulé, G., Fernández Pinto, V., Moretti, A., Phylogenetic analysis of a population of Fusarium graminearum from Argentina (2006) European Fusarium Seminar, , The Netherlands
- Andrews, S., Pitt, J.I., Selective medium for isolation of Fusarium species and dematiaceous Hyphomycetes from cereals (1987) Appl Environ Microbiol, 51, pp. 1235-8
- Nelson, P.E., Toussoun, T.A., Marasas, W.F.O., (1983) Fusarium Species: An Illustrated Manual for Identification, , The Pennsylvania State University Park Pennsylvania
- Pitt, J.I., Hocking, A.D., (1997) Fungi and Food Spoilage, , Blackie Academic & Professional London
- Samson, R.A., Hoekstra, E.S., Frisvad, J.C., Filtenborg, O., (2000) Introduction to Foodborne Fungi, , Centraalbureau voor Schimmelcultures Baarn
- Klich, M.A., (2002) Identification of Common Aspergillus Species, , Centraalbureau voor Schimmelcultures Baarn
- Trucksess, M.W., Ready, D.W., Pender, M.K., Ligmond, C.A., Wood, G.E., Page, S.W., Determination and survey of deoxynivalenol in white flour, whole wheat flour, and bran (1996) J AOAC Int, 74, pp. 883-7
- Samar, M., Ferro Fontán, C., Resnik, S.L., Pacin, A., Castillo, M., Distribution of deoxynivalenol in wheat, wheat flour, bran, and gluten, and variability associated with the test procedure (2003) J AOAC Int, 86, pp. 551-6
- Croteau, S.M., Prelusky, D.B., Trenholm, H.L., Analysis of trichothecenes mycotoxins by gas chromatography with electron capture detection (1994) J Agric Food Chem, 42, pp. 928-34
- Conover, W.J., (1980) Practical Nonparametric Statistics, , John Wiley & Sons New York
- Devore, J.L., (1987) Probability and Statistics for Engineering and the Sciences, , Brooks-Cole Publishing Company Monterrey
- Pagano, M., Gauvreau, K., (2000) Principles of Bioestatistics, , Duxbury Press Pacific Grove
- (1995) GEMS/Food EURO Second Workshop on Reliable Evaluation of Low Level Contamination of Food, , Kulmbach, Germany, 26-27 May
- (2000) Statistix Version 7.0. Analytical Software, , Tallahassee, Florida
- Broggi, L.E., González, H.H.L., Resnik, S.L., Pacin, A., Alternaria alternata prevalence in cereals grains and soybean seeds from Entre Ríos, Argentina (2007) Revista Iberoamericana de Micología, 24, pp. 47-51
- Torp, M., Nirenberg, H., Fusarium langsethiae sp. nov. on cereals in Europe (2004) Int J Food Microbiol, 95, pp. 247-56
- Henriksen, B., Elen, O., Natural Fusarium grain infection level in wheat, barley and oat after early application of fungicides and herbicides (2005) J Phytopathol, 153, pp. 214-20
- Roháčik, T., Hudec, K., Influence of agro-environmental factors on Fusarium infestation and population structure in wheat kernels (2005) Ann Agric Environ Med, 12, pp. 39-45
- Schollenberger, M., Müller, H.M., Rüfle, M., Suchy, S., Plank, S., Drochner, W., Natural occurrence of 16 Fusarium toxins in grains and feedstuffs of plant origin from Germany (2006) Mycopathologia, 161, pp. 43-52
Citas:
---------- APA ----------
González, H.H.L., Moltó, G.A., Pacin, A., Resnik, S.L., Zelaya, M.J., Masana, M. & Martínez, E.J.
(2008)
. Trichothecenes and mycoflora in wheat harvested in nine locations in Buenos Aires Province, Argentina. Mycopathologia, 165(2), 105-114.
http://dx.doi.org/10.1007/s11046-007-9084-x---------- CHICAGO ----------
González, H.H.L., Moltó, G.A., Pacin, A., Resnik, S.L., Zelaya, M.J., Masana, M., et al.
"Trichothecenes and mycoflora in wheat harvested in nine locations in Buenos Aires Province, Argentina"
. Mycopathologia 165, no. 2
(2008) : 105-114.
http://dx.doi.org/10.1007/s11046-007-9084-x---------- MLA ----------
González, H.H.L., Moltó, G.A., Pacin, A., Resnik, S.L., Zelaya, M.J., Masana, M., et al.
"Trichothecenes and mycoflora in wheat harvested in nine locations in Buenos Aires Province, Argentina"
. Mycopathologia, vol. 165, no. 2, 2008, pp. 105-114.
http://dx.doi.org/10.1007/s11046-007-9084-x---------- VANCOUVER ----------
González, H.H.L., Moltó, G.A., Pacin, A., Resnik, S.L., Zelaya, M.J., Masana, M., et al. Trichothecenes and mycoflora in wheat harvested in nine locations in Buenos Aires Province, Argentina. Mycopathologia. 2008;165(2):105-114.
http://dx.doi.org/10.1007/s11046-007-9084-x