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Zubeldia-Brenner, L.; Gutierrez-Uzquiza, A.; Barrio-Real, L.; Wang, H.; Kazanietz, M.G.; Leskow, F.C. "β3-Chimaerin, a novel member of the chimaerin Rac-GAP family" (2014) Molecular Biology Reports. 41(4):2067-2076
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Abstract:

Chimaerins are a family of diacylglycerol- and phorbol ester-regulated GTPase activating proteins (GAPs) for the small G-protein Rac. Extensive evidence indicates that these proteins play important roles in development, axon guidance, metabolism, cell motility, and T cell activation. Four isoforms have been reported to-date, which are products of CHN1 (α1- and α2-chimaerins) and CHN2 (β1- and β2-chimaerins) genes. Although these gene products are assumed to be generated by alternative splicing, bioinformatics analysis of the CHN2 gene revealed that β1- and β2-chimaerins are the products of alternative transcription start sites (TSSs) in different promoter regions. Furthermore, we found an additional TSS in CHN2 gene that leads to a novel product, which we named β3-chimaerin. Expression profile analysis revealed predominantly low levels for the β3-chimaerin transcript, with higher expression levels in epididymis, plasma blood leucocytes, spleen, thymus, as well as various areas of the brain. In addition to the prototypical SH2, C1, and Rac-GAP domains, β3-chimaerin has a unique N-terminal domain. Studies in cells established that β3-chimaerin has Rac-GAP activity and is responsive to phorbol esters. The enhanced responsiveness of β3-chimaerin for phorbol ester-induced translocation relative to β2-chimaerin suggests differential ligand accessibility to the C1 domain. © 2014 Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:β3-Chimaerin, a novel member of the chimaerin Rac-GAP family
Autor:Zubeldia-Brenner, L.; Gutierrez-Uzquiza, A.; Barrio-Real, L.; Wang, H.; Kazanietz, M.G.; Leskow, F.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina
Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6160, United States
Departamento de Ciencias Básicas, Universidad Nacional de Luján, 6700 Luján Buenos Aires, Argentina
Palabras clave:C1 domain; Chimaerin; CHN2; Phorbol esters; Rac-GAP; alpha1 chimaerin; alpha2 chimaerin; beta1 chimaerin; beta2 chimaerin; beta3 chimaerin; chimerin; guanosine triphosphatase activating protein; isoprotein; phorbol ester; unclassified drug; chimaerin-beta3 protein, human; chimerin; isoprotein; phorbol 13 acetate 12 myristate; protein binding; Rac protein; alternative RNA splicing; amino terminal sequence; article; bioinformatics; CHN1 gene; CHN2 gene; controlled study; enzyme activity; enzyme analysis; epididymis; exon; genetic analysis; human; human cell; human tissue; leukocyte; nucleotide sequence; promoter region; protein analysis; protein domain; protein expression; spleen; thymus; tissue distribution; transcription initiation site; amino acid sequence; animal; antibody specificity; cell line; chemistry; Chlorocebus aethiops; COS 1 cell line; drug effects; enzyme activation; gene expression; gene expression profiling; gene expression regulation; gene order; genetics; metabolism; molecular genetics; Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cercopithecus aethiops; Chimerin Proteins; COS Cells; Enzyme Activation; Gene Expression; Gene Expression Profiling; Gene Expression Regulation; Gene Order; Humans; Molecular Sequence Data; Organ Specificity; Promoter Regions, Genetic; Protein Binding; Protein Interaction Domains and Motifs; Protein Isoforms; rac GTP-Binding Proteins; Tetradecanoylphorbol Acetate
Año:2014
Volumen:41
Número:4
Página de inicio:2067
Página de fin:2076
DOI: http://dx.doi.org/10.1007/s11033-014-3055-3
Título revista:Molecular Biology Reports
Título revista abreviado:Mol. Biol. Rep.
ISSN:03014851
CODEN:MLBRB
CAS:phorbol 13 acetate 12 myristate, 16561-29-8; chimaerin-beta3 protein, human; Chimerin Proteins; Protein Isoforms; rac GTP-Binding Proteins; Tetradecanoylphorbol Acetate
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014851_v41_n4_p2067_ZubeldiaBrenner

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Citas:

---------- APA ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G. & Leskow, F.C. (2014) . β3-Chimaerin, a novel member of the chimaerin Rac-GAP family. Molecular Biology Reports, 41(4), 2067-2076.
http://dx.doi.org/10.1007/s11033-014-3055-3
---------- CHICAGO ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G., Leskow, F.C. "β3-Chimaerin, a novel member of the chimaerin Rac-GAP family" . Molecular Biology Reports 41, no. 4 (2014) : 2067-2076.
http://dx.doi.org/10.1007/s11033-014-3055-3
---------- MLA ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G., Leskow, F.C. "β3-Chimaerin, a novel member of the chimaerin Rac-GAP family" . Molecular Biology Reports, vol. 41, no. 4, 2014, pp. 2067-2076.
http://dx.doi.org/10.1007/s11033-014-3055-3
---------- VANCOUVER ----------
Zubeldia-Brenner, L., Gutierrez-Uzquiza, A., Barrio-Real, L., Wang, H., Kazanietz, M.G., Leskow, F.C. β3-Chimaerin, a novel member of the chimaerin Rac-GAP family. Mol. Biol. Rep. 2014;41(4):2067-2076.
http://dx.doi.org/10.1007/s11033-014-3055-3