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Abstract:

The dimerization and aggregation of ribosomal particles, an artifact produced by glutaraldehyde fixation, is described. This effect is especially noticeable with 30S subunits. The influence of ribosomal, Mg++ and fixative reagent concentrations has been studied in order to find conditions adequate to diminish the deleterious effects of the ribosomal fixation. © 1976 D. Reidel Publishing Company.

Registro:

Documento: Artículo
Título:Artifacts induced by glutaraldehyde fixation of ribosomal particles
Autor:Garcia-Patrone, M.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioquimicas 'Fundación Campomar', and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:glutaraldehyde; magnesium; article; binding site; density gradient centrifugation; Escherichia coli; kinetics; ribosome; staining; ultrastructure; Binding Sites; Centrifugation, Density Gradient; Escherichia coli; Glutaral; Kinetics; Magnesium; Ribosomes; Staining and Labeling
Año:1976
Volumen:2
Número:6
Página de inicio:507
Página de fin:515
DOI: http://dx.doi.org/10.1007/BF00356940
Título revista:Molecular Biology Reports
Título revista abreviado:Mol. Biol. Rep.
ISSN:03014851
CODEN:MLBRB
CAS:glutaraldehyde, 111-30-8, 37245-61-7; magnesium, 7439-95-4; Glutaral, 111-30-8; Magnesium, 7439-95-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014851_v2_n6_p507_GarciaPatrone

Referencias:

  • Spirin, A.S., (1971) FEBS Letters, 14, p. 349
  • Spirin, A.S., Belitsina, N.V., Lishnevskaya, E.B., (1972) FEBS Letters, 24, p. 219
  • Infante, A.A., Baierlein, R., (1971) Proc. Nat. Acad. Sci. U.S.A., 68, p. 1780
  • Subramanian, A.R., Davis, B.D., (1971) Proc. Nat. Acad. Sci. U.S.A., 68, p. 2453
  • Liautard, J.P., Lecon, C., Köhler, K., (1973) Biochimie, 55, p. 877
  • Cocito, C., (1973) Biochimie, 55, p. 309
  • Leick, V., Engberg, J., Emmersen, J., (1970) Eur. J. Biochem., 13, p. 238
  • Spirin, A.S., Belitsina, N.V., Lerman, N.I., (1965) J. Mol. Biol., 14, p. 611
  • Subramanian, A.R., (1972) Biochemistry, 11, p. 2710
  • Baltimore, D., Huang, A.S., (1968) Science, 162, p. 572
  • Sabatini, D.D., Bensch, K., Barnett, R.J., (1963) J. Cell Biol., 17, p. 19
  • Sun, T.T., Traut, R.R., Kahan, L., (1974) J. Mol. Biol., 87, p. 509
  • Garcia-Patrone, M., González, N.S., Algranati, I.D., (1972) FEBS Letters, 24, p. 126
  • Traub, P., Nomura, M., (1968) J. Mol. Biol., 34, p. 575
  • Garcia-Patrone, M., González, N.S., Algranati, I.D., (1975) Biochim. Biophys. Acta, 395, p. 373
  • Gavrilova, L.P., Spirin, A.S., (1974) Methods Enzymol., 30, p. 452

Citas:

---------- APA ----------
Garcia-Patrone, M. & Algranati, I.D. (1976) . Artifacts induced by glutaraldehyde fixation of ribosomal particles. Molecular Biology Reports, 2(6), 507-515.
http://dx.doi.org/10.1007/BF00356940
---------- CHICAGO ----------
Garcia-Patrone, M., Algranati, I.D. "Artifacts induced by glutaraldehyde fixation of ribosomal particles" . Molecular Biology Reports 2, no. 6 (1976) : 507-515.
http://dx.doi.org/10.1007/BF00356940
---------- MLA ----------
Garcia-Patrone, M., Algranati, I.D. "Artifacts induced by glutaraldehyde fixation of ribosomal particles" . Molecular Biology Reports, vol. 2, no. 6, 1976, pp. 507-515.
http://dx.doi.org/10.1007/BF00356940
---------- VANCOUVER ----------
Garcia-Patrone, M., Algranati, I.D. Artifacts induced by glutaraldehyde fixation of ribosomal particles. Mol. Biol. Rep. 1976;2(6):507-515.
http://dx.doi.org/10.1007/BF00356940