Abstract:
Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoid carcinoma) and A-427 (lung adenocarcinoma) on peripheral blood monocytes (MO) from 20 healthy donors (HD). Methods and results: MO were incubated with supernatants or with medium only (controls) during 2 or 18 hours. There were determined the expression of HLA-DR antigen by indirect immunofluorescence technique, the formation of reactive oxygen intermediates (NBT reduction capacity) and the phagocytic capacity (Candida albicans-anti Candida albicans system). These determinations were made also in MO from 16 patients with advanced lung cancer (LCP). Percentages of expression (media ± SE) were: HLA-DR (+) MO: Calu-1 = 30 ± 2; A-427 = 31 ± 2; LCP = 52 ± 3 (p < 0.0001 vs HD: 77 ± 1%) NBT (+)MO: Calu-1 = 47 ± 1; A-427 = 49 ± 1; LCP = 56 ± 2 (p < 0.01 vs HD: 45 ± 2%), Phagocytic MO: Calu-1 = 38 ± 3; A-427 = 39 ± 2; LCP = 32 ± 3 (p < 0.0001 vs HD: 50 ± 1%). Conclusions: we conclude that this in vitro model reproduced characteristics found in MO from lung cancer patients, since culture supernatants induced in normal MO phenotypic and functional characteristics also found in MO from patients analyzed.
Registro:
Documento: |
Artículo
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Título: | Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes |
Autor: | Novellino, P.S.; Trejo, Y.G.; Beviacqua, M.; Bordenave, R.H.; Rumi, L.S. |
Filiación: | Laboratorio de Inmunología., Inst. de Biol. y Med. Exp. (IByME), Buenos Aires, Argentina Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
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Palabras clave: | Culture supernatants; Lung cancer cell lines; Monocytes; HLA DR antigen; article; cell culture; culture medium; drug effect; fluorescent antibody technique; human; monocyte; phagocytosis; secretion; Culture Media, Conditioned; Fluorescent Antibody Technique, Indirect; HLA-DR Antigens; Humans; Monocytes; Phagocytosis; Tumor Cells, Cultured |
Año: | 1999
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Volumen: | 27
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Número: | 4
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Página de inicio: | 195
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Página de fin: | 199
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Título revista: | Allergologia et Immunopathologia
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Título revista abreviado: | Allergol. Immunopathol.
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ISSN: | 03010546
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CODEN: | AGIMB
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CAS: | Culture Media, Conditioned; HLA-DR Antigens
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino |
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Citas:
---------- APA ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H. & Rumi, L.S.
(1999)
. Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes. Allergologia et Immunopathologia, 27(4), 195-199.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]
---------- CHICAGO ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S.
"Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes"
. Allergologia et Immunopathologia 27, no. 4
(1999) : 195-199.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]
---------- MLA ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S.
"Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes"
. Allergologia et Immunopathologia, vol. 27, no. 4, 1999, pp. 195-199.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]
---------- VANCOUVER ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S. Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes. Allergol. Immunopathol. 1999;27(4):195-199.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]