Artículo

Novellino, P.S.; Trejo, Y.G.; Beviacqua, M.; Bordenave, R.H.; Rumi, L.S. "Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes" (1999) Allergologia et Immunopathologia. 27(4):195-199
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Abstract:

Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoid carcinoma) and A-427 (lung adenocarcinoma) on peripheral blood monocytes (MO) from 20 healthy donors (HD). Methods and results: MO were incubated with supernatants or with medium only (controls) during 2 or 18 hours. There were determined the expression of HLA-DR antigen by indirect immunofluorescence technique, the formation of reactive oxygen intermediates (NBT reduction capacity) and the phagocytic capacity (Candida albicans-anti Candida albicans system). These determinations were made also in MO from 16 patients with advanced lung cancer (LCP). Percentages of expression (media ± SE) were: HLA-DR (+) MO: Calu-1 = 30 ± 2; A-427 = 31 ± 2; LCP = 52 ± 3 (p < 0.0001 vs HD: 77 ± 1%) NBT (+)MO: Calu-1 = 47 ± 1; A-427 = 49 ± 1; LCP = 56 ± 2 (p < 0.01 vs HD: 45 ± 2%), Phagocytic MO: Calu-1 = 38 ± 3; A-427 = 39 ± 2; LCP = 32 ± 3 (p < 0.0001 vs HD: 50 ± 1%). Conclusions: we conclude that this in vitro model reproduced characteristics found in MO from lung cancer patients, since culture supernatants induced in normal MO phenotypic and functional characteristics also found in MO from patients analyzed.

Registro:

Documento: Artículo
Título:Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
Autor:Novellino, P.S.; Trejo, Y.G.; Beviacqua, M.; Bordenave, R.H.; Rumi, L.S.
Filiación:Laboratorio de Inmunología., Inst. de Biol. y Med. Exp. (IByME), Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Culture supernatants; Lung cancer cell lines; Monocytes; HLA DR antigen; article; cell culture; culture medium; drug effect; fluorescent antibody technique; human; monocyte; phagocytosis; secretion; Culture Media, Conditioned; Fluorescent Antibody Technique, Indirect; HLA-DR Antigens; Humans; Monocytes; Phagocytosis; Tumor Cells, Cultured
Año:1999
Volumen:27
Número:4
Página de inicio:195
Página de fin:199
Título revista:Allergologia et Immunopathologia
Título revista abreviado:Allergol. Immunopathol.
ISSN:03010546
CODEN:AGIMB
CAS:Culture Media, Conditioned; HLA-DR Antigens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino

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Citas:

---------- APA ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H. & Rumi, L.S. (1999) . Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes. Allergologia et Immunopathologia, 27(4), 195-199.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]
---------- CHICAGO ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S. "Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes" . Allergologia et Immunopathologia 27, no. 4 (1999) : 195-199.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]
---------- MLA ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S. "Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes" . Allergologia et Immunopathologia, vol. 27, no. 4, 1999, pp. 195-199.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]
---------- VANCOUVER ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S. Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes. Allergol. Immunopathol. 1999;27(4):195-199.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino [ ]