Artículo
Segretin, M.E.; Galeano, A.; Roldán, A.; Schillaci, R. "Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes" (2003) Hormone Research. 59(6):276-280
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Abstract:
Objective: To investigate the kinetics of insulin-like growth factor-1 receptor (IGF-1R) expression in PHA-stimulated T lymphocytes. Methods: IGF-1R protein and mRNA were detected by flow cytometry and RT-PCR respectively, between 0 and 48 h after cell activation. Results: Few minutes after T lymphocytes were activated, internalization of the IGF-1R from the cell membrane was observed, achieving the lower level between 1 and 6 h and was accompanied by a reduction in its mRNA. This was followed by re-expression of IGF-1R on the cell surface and an increase in IGF-1R mRNA levels in the cytoplasm, reaching levels higher than those recorded initially after 48 h activation. Conclusion: This down- and up-regulation suggests that restoration of IGF-1R would be the result of receptor recycling and de novo synthesis and highlights its importance for T lymphocyte proliferation. Copyright © 2003 S. Karger AG, Basel.
Registro:
| Documento: | Artículo |
| Título: | Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes |
| Autor: | Segretin, M.E.; Galeano, A.; Roldán, A.; Schillaci, R. |
| Filiación: | Inst. de Biol. y Med. Exp. CONICET, Buenos Aires, Argentina Lab. de Patol. Sanatorio Mater Dei, Buenos Aires, Argentina Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | Down-regulation; Insulin-like growth factor-1 receptor; T lymphocyte; Up-regulation; messenger RNA; phytohemagglutinin; somatomedin C receptor; article; cell membrane; cell surface; cell transport; flow cytometry; human; human cell; kinetics; lymphocyte proliferation; priority journal; protein expression; receptor upregulation; reverse transcription polymerase chain reaction; T lymphocyte; T lymphocyte activation; Down-Regulation; Flow Cytometry; Humans; Kinetics; Lymphocyte Activation; Receptor, IGF Type 1; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; T-Lymphocytes; Up-Regulation |
| Año: | 2003 |
| Volumen: | 59 |
| Número: | 6 |
| Página de inicio: | 276 |
| Página de fin: | 280 |
| DOI: | http://dx.doi.org/10.1159/000070625 |
| Título revista: | Hormone Research |
| Título revista abreviado: | Horm. Res. |
| ISSN: | 03010163 |
| CODEN: | HRMRA |
| CAS: | phytohemagglutinin, 9008-97-3; Receptor, IGF Type 1, EC 2.7.1.112; RNA, Messenger |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010163_v59_n6_p276_Segretin |
Referencias:
- Brocardo, M.G., Schillaci, R., Galeano, A., Radrizzani, M., White, V., González Gerrico, A., Santa-Coloma, T.A., Roldán, A., Early effects of IGF-1 in activated human T lymphocytes (2001) J Leuk Biol, 70, pp. 297-305
- Zapf, A., Hsu, D., Olefsky, J.M., Comparison of the intracellular itineraries of insulin-like growth factor-I and insulin and their receptors in Rat-1 fibroblasts (1994) Endocrinology, 134, pp. 2445-2452
- Schillaci, R., Brocardo, M.G., Galeano, A., Roldán, A., Down-regulation of insulin like-growth factor-1 receptor (IGF-1R) expression in human T lymphocytes activation (1998) Cell Immunol, 183, pp. 157-161
- Johnson, E.W., Jones, L.A., Kozak, R.W., Expression and function of insulin-like growth factor receptors on anti-CD3-activated human T lymphocytes (1992) J Immunol, 148, pp. 63-71
- Walsh, P.T., O'Connor, R., The insulin-like growth factor-I receptor is regulated by CD28 and protects activated T cells from apoptosis (2000) Eur J Immunol, 30, pp. 1010-1018
- Tapson, V.F., Boni-Schnetzler, M., Pilch, P.F., Center, D.M., Berman, J.S., Structural and functional characterization of the human T lymphocyte receptor for insulin-like growth factor I in vitro (1988) J Clin Invest, 82, pp. 950-957
- Quinn, K.A., Treston, A.M., Unsworth, E.J., Miller, M.-J., Vos, M., Grimley, C., Battey, J., Cuttitta, F.J., Insulin-like growth factor expression in human cancer cell lines (1996) J Biol Chem, 271, pp. 11477-11483
- Zhou, L., Ritchie, D., Wang, E.Y., Barbone, A.G., Argentieri, D., Lau, C.Y., Tepoxalin, a novel immunosuppressive agent with a different mechanism of action from cyclosporin A (1994) J Immunol, 153, pp. 5026-5037
- Hartmann, K.K.P., Baier, T.G., Papa, V., Kronenwett, M.E., Brown, J., Golfine, I.R., Rosenthal, S.M.J., A monoclonal antibody to the T-cell receptor increases IGF-I receptor content in normal T-lymphocytes: Comparison with phytohemagglutinin (1992) Cell Biochem, 48, pp. 81-85
- Reiss, K., Porcu, P., Sell, C., Pietrzkowski, Z., Baserga, R., The insulin-like growth factor 1 receptor is required for the proliferation of hemopoietic cells (1992) Oncogene, 7, pp. 2243-2248
- Condorelli, G., Bueno, R., Smith, R.J., Two alternatively spliced forms of the human insulin-like growth factor I receptor have distinct biological activities and internalization kinetics (1994) J Biol Chem, 269, pp. 8510-8516
Citas:
---------- APA ----------
Segretin, M.E., Galeano, A., Roldán, A. & Schillaci, R. (2003) . Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes. Hormone Research, 59(6), 276-280.http://dx.doi.org/10.1159/000070625
---------- CHICAGO ----------
Segretin, M.E., Galeano, A., Roldán, A., Schillaci, R. "Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes" . Hormone Research 59, no. 6 (2003) : 276-280.http://dx.doi.org/10.1159/000070625
---------- MLA ----------
Segretin, M.E., Galeano, A., Roldán, A., Schillaci, R. "Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes" . Hormone Research, vol. 59, no. 6, 2003, pp. 276-280.http://dx.doi.org/10.1159/000070625
---------- VANCOUVER ----------
Segretin, M.E., Galeano, A., Roldán, A., Schillaci, R. Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes. Horm. Res. 2003;59(6):276-280.http://dx.doi.org/10.1159/000070625
