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Santa-Coloma, T.A.; Muschietti, J.P.; Charreau, E.H. "Sex steroid binding protein from Bufo arenarum: Further characterization" (1986) Comparative Biochemistry and Physiology -- Part A: Physiology. 85(3):401-405
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Abstract:

1. 1. The effects of temperature, pH, divalent cations, 2-mercaptoethanol (Et-SH), N-ethylmaleimide (NEM), and phenylmethylsulfonyl fluoride (PMSF) on the dihydrotestosterone (DHT) binding to sex steroid binding protein from Bufo arenarum (baSBP) were examined. 2. 2. The temperature curve indicated that the binding remained stable up to 50°C and the pH curve showed maximum binding between pH 7 and 9. 3. 3. The incubations of baSBP with divalent cations, NEM and Et-SH demonstrated that baSBP require disulfides and sulfhydryl groups for steroid binding or to maintain an adequate protein conformation. 4. 4. On the other hand, PMSF had no effect on the binding, consequently, serine residues appear not to be involved in DHT binding to baSBP. 5. 5. These results indicate that baSBP has a behavior resembling that of human SBP. © 1986.

Registro:

Documento: Artículo
Título:Sex steroid binding protein from Bufo arenarum: Further characterization
Autor:Santa-Coloma, T.A.; Muschietti, J.P.; Charreau, E.H.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:benzylsulfonyl fluoride; mercaptoethanol; n ethylmaleimide; radioisotope; androstanolone h 3; animal cell; drug protein binding; nonhuman; priority journal; Animal; Bufo arenarum; Female; Hydrogen-Ion Concentration; Kinetics; Sex Hormone-Binding Globulin; Stanolone; Thermodynamics
Año:1986
Volumen:85
Número:3
Página de inicio:401
Página de fin:405
DOI: http://dx.doi.org/10.1016/0300-9629(86)90420-2
Título revista:Comparative Biochemistry and Physiology -- Part A: Physiology
ISSN:03009629
CAS:benzylsulfonyl fluoride, 329-98-6; mercaptoethanol, 60-24-2; n ethylmaleimide, 128-53-0; Sex Hormone-Binding Globulin; Stanolone, 521-18-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009629_v85_n3_p401_SantaColoma

Referencias:

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Citas:

---------- APA ----------
Santa-Coloma, T.A., Muschietti, J.P. & Charreau, E.H. (1986) . Sex steroid binding protein from Bufo arenarum: Further characterization. Comparative Biochemistry and Physiology -- Part A: Physiology, 85(3), 401-405.
http://dx.doi.org/10.1016/0300-9629(86)90420-2
---------- CHICAGO ----------
Santa-Coloma, T.A., Muschietti, J.P., Charreau, E.H. "Sex steroid binding protein from Bufo arenarum: Further characterization" . Comparative Biochemistry and Physiology -- Part A: Physiology 85, no. 3 (1986) : 401-405.
http://dx.doi.org/10.1016/0300-9629(86)90420-2
---------- MLA ----------
Santa-Coloma, T.A., Muschietti, J.P., Charreau, E.H. "Sex steroid binding protein from Bufo arenarum: Further characterization" . Comparative Biochemistry and Physiology -- Part A: Physiology, vol. 85, no. 3, 1986, pp. 401-405.
http://dx.doi.org/10.1016/0300-9629(86)90420-2
---------- VANCOUVER ----------
Santa-Coloma, T.A., Muschietti, J.P., Charreau, E.H. Sex steroid binding protein from Bufo arenarum: Further characterization. 1986;85(3):401-405.
http://dx.doi.org/10.1016/0300-9629(86)90420-2