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Abstract:

The adrenergic regulation of the low-Km pineal cAMP phosphodiesterase (PDE) activity was studied in adult female rats. PDE activity showed a transient enhancement (up to 42%) during the process of degeneration of pineal sympathetic nerve terminals that followed superior cervical ganglionectomy (SCGx), thus confirming the neural modulation of the enzyme. Treatment with isoproterenol (0.3-5.0 mg/Kg) increased significantly PDE activity within 2 hours. Phenylephrine induced a significant increase of pineal PDE only at a 10 mg/Kg dose, while at a lower dose (1 mg/Kg) it potenciated the stimulatory effect of isoproterenol. Treatment of pineal organ cultures with 100 ΜM propranolol inhibited norepinephrine (NE)-induced PDE activity while 100 ΜM phentolamine had no significant effect. Propranolol at doses unable to alter the in vitro NE-induced stimulation of pineal PDE activity (1 ΜM), antagonized such NE effect when used in combination with 1 uM phentolamine. At equimolecular concentrations (1 ΜM) the mixed α-Β-adrenergic agonist NE was more effective than the Β-adrenergic agonist isoproterenol to increase pineal PDE in vitro. These results © 1985 Springer-Verlag.

Registro:

Documento: Artículo
Título:Interaction between α- and β-adrenoceptors in rat pineal adenosine cyclic 3′,5′-monophosphate phosphodiesterase activation
Autor:Vacas, M.I.; Sarmiento, M.I.K.; Cardinali, D.P.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Buenos Aires, Argentina
Palabras clave:alpha adrenergic receptor; beta adrenergic receptor; cyclic amp phosphodiesterase; enzyme; isoprenaline; noradrenalin; phentolamine; phenylephrine; propranolol; radioisotope; animal cell; animal experiment; autonomic nervous system; biological model; central nervous system; cyclic amp h 3; dose response; drug activation; drug comparison; drug inhibition; drug potentiation; drug response; endocrine system; enzyme activation; ganglionectomy; intraperitoneal drug administration; nervous system; nonhuman; pineal body; priority journal; rat; superior cervical ganglion; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Animal; Female; Ganglia, Sympathetic; In Vitro; Isoproterenol; Norepinephrine; Phentolamine; Phenylephrine; Pineal Gland; Propranolol; Rats; Rats, Inbred Strains; Receptors, Adrenergic, alpha; Receptors, Adrenergic, beta; Support, Non-U.S. Gov't
Año:1985
Volumen:62
Número:3-4
Página de inicio:295
Página de fin:304
DOI: http://dx.doi.org/10.1007/BF01252243
Título revista:Journal of Neural Transmission
Título revista abreviado:J. Neural Transmission
ISSN:03009564
CODEN:JNTRF
CAS:cyclic AMP phosphodiesterase, 9036-21-9; isoprenaline, 299-95-6, 51-30-9, 6700-39-6, 7683-59-2; noradrenalin, 1407-84-7, 51-41-2; phentolamine, 50-60-2, 73-05-2; phenylephrine, 532-38-7, 59-42-7, 61-76-7; propranolol, 13013-17-7, 318-98-9, 3506-09-0, 4199-09-1, 525-66-6; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Isoproterenol, 7683-59-2; Norepinephrine, 51-41-2; Phentolamine, 50-60-2; Phenylephrine, 59-42-7; Propranolol, 525-66-6; Receptors, Adrenergic, alpha; Receptors, Adrenergic, beta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009564_v62_n3-4_p295_Vacas

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Citas:

---------- APA ----------
Vacas, M.I., Sarmiento, M.I.K. & Cardinali, D.P. (1985) . Interaction between α- and β-adrenoceptors in rat pineal adenosine cyclic 3′,5′-monophosphate phosphodiesterase activation. Journal of Neural Transmission, 62(3-4), 295-304.
http://dx.doi.org/10.1007/BF01252243
---------- CHICAGO ----------
Vacas, M.I., Sarmiento, M.I.K., Cardinali, D.P. "Interaction between α- and β-adrenoceptors in rat pineal adenosine cyclic 3′,5′-monophosphate phosphodiesterase activation" . Journal of Neural Transmission 62, no. 3-4 (1985) : 295-304.
http://dx.doi.org/10.1007/BF01252243
---------- MLA ----------
Vacas, M.I., Sarmiento, M.I.K., Cardinali, D.P. "Interaction between α- and β-adrenoceptors in rat pineal adenosine cyclic 3′,5′-monophosphate phosphodiesterase activation" . Journal of Neural Transmission, vol. 62, no. 3-4, 1985, pp. 295-304.
http://dx.doi.org/10.1007/BF01252243
---------- VANCOUVER ----------
Vacas, M.I., Sarmiento, M.I.K., Cardinali, D.P. Interaction between α- and β-adrenoceptors in rat pineal adenosine cyclic 3′,5′-monophosphate phosphodiesterase activation. J. Neural Transmission. 1985;62(3-4):295-304.
http://dx.doi.org/10.1007/BF01252243