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Abstract:

Insulin-like growth factor-1 (IGF-I) is an essential factor for the differentiation of preadipocytes into adipocytes. We investigated the expression of IGF-I receptor and IGF-I RNA messenger during 3T3-L1 preadipocyte differentiation. Levels of IGF-I receptor decreased in the mature adipocytes compared to cells before the initiation of differentiation. In addition, cultures not induced to differentiate showed a decrease on the receptor levels after 4 days in the presence of insulin compared to cultures without treatment. The levels of the IGF-I RNA messenger were shown to be higher in mature adipocytes compared to preadipocytes. We propose an autocrine and/or paracrine action of IGF-I in this adipocyte differentiation model, where IGF-I produced by the differentiating preadipocytes acts over their adjacent cells and, in this way, diminishes the expression of IGF-I receptor. © 2002 Éditions scientifiques et médicales Elsevier SAS and Société française de biochimie et biologie moléculaire. All rights reserved.

Registro:

Documento: Artículo
Título:Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes
Autor:Zizola, C.F.; Balaña, M.E.; Sandoval, M.; Calvo, J.C.
Filiación:Inst. de Biol. Med. Exp. (CONICET), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Departamento de Quimica Biologica, Facul. de Ciencias Exactas Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Adipocytes; Differentiation; IGF-I receptor; Insulin-like growth factor-1; insulin; messenger RNA; somatomedin C; somatomedin C receptor; adipocyte; animal cell; article; autocrine effect; cell culture; cell differentiation; cell maturation; controlled study; embryo; in vitro study; model; mouse; nonhuman; paracrine signaling; proadipocyte; protein expression; protein synthesis; 3T3 Cells; Adipocytes; Animals; Cell Differentiation; Gene Expression Regulation; Insulin; Insulin-Like Growth Factor I; Mice; Receptor, IGF Type 1; RNA, Messenger
Año:2002
Volumen:84
Número:10
Página de inicio:975
Página de fin:980
DOI: http://dx.doi.org/10.1016/S0300-9084(02)00009-3
Título revista:Biochimie
Título revista abreviado:Biochimie
ISSN:03009084
CODEN:BICMB
CAS:insulin, 9004-10-8; somatomedin C, 67763-96-6; Insulin, 11061-68-0; Insulin-Like Growth Factor I, 67763-96-6; Receptor, IGF Type 1, EC 2.7.1.112; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009084_v84_n10_p975_Zizola

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Citas:

---------- APA ----------
Zizola, C.F., Balaña, M.E., Sandoval, M. & Calvo, J.C. (2002) . Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes. Biochimie, 84(10), 975-980.
http://dx.doi.org/10.1016/S0300-9084(02)00009-3
---------- CHICAGO ----------
Zizola, C.F., Balaña, M.E., Sandoval, M., Calvo, J.C. "Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes" . Biochimie 84, no. 10 (2002) : 975-980.
http://dx.doi.org/10.1016/S0300-9084(02)00009-3
---------- MLA ----------
Zizola, C.F., Balaña, M.E., Sandoval, M., Calvo, J.C. "Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes" . Biochimie, vol. 84, no. 10, 2002, pp. 975-980.
http://dx.doi.org/10.1016/S0300-9084(02)00009-3
---------- VANCOUVER ----------
Zizola, C.F., Balaña, M.E., Sandoval, M., Calvo, J.C. Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes. Biochimie. 2002;84(10):975-980.
http://dx.doi.org/10.1016/S0300-9084(02)00009-3