Artículo
Ulloa, R.M.; Glikin, G.C.; Téllez-Ĩnón, M.T.; Torres, H.N.; Judewicz, N.D. "A novel stimulator of protein phosphorylation in Neurospora crassa" (1987) Molecular and Cellular Biochemistry. 77(1):11-17
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Abstract:
An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed. © 1987 Martinus Nijhoff Publishers.
Registro:
| Documento: | Artículo |
| Título: | A novel stimulator of protein phosphorylation in Neurospora crassa |
| Autor: | Ulloa, R.M.; Glikin, G.C.; Téllez-Ĩnón, M.T.; Torres, H.N.; Judewicz, N.D. |
| Filiación: | Instituto, de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI) CONICET, Obligado 2490, Buenos Aires, 1428, Argentina Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina |
| Palabras clave: | Neurospora crassa; protein kinase regulation; fungal protein; protein kinase; article; enzyme activation; enzymology; gel chromatography; growth, development and aging; kinetics; metabolism; molecular weight; Neurospora; Neurospora crassa; phosphorylation; Chromatography, Gel; Enzyme Activation; Fungal Proteins; Kinetics; Molecular Weight; Neurospora; Neurospora crassa; Phosphorylation; Protein Kinases |
| Año: | 1987 |
| Volumen: | 77 |
| Número: | 1 |
| Página de inicio: | 11 |
| Página de fin: | 17 |
| DOI: | http://dx.doi.org/10.1007/BF00230146 |
| Título revista: | Molecular and Cellular Biochemistry |
| Título revista abreviado: | Mol Cell Biochem |
| ISSN: | 03008177 |
| CODEN: | MCBIB |
| CAS: | protein kinase, 9026-43-1; Fungal Proteins; Protein Kinases, EC 2.7.1.37 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v77_n1_p11_Ulloa |
Referencias:
- Nimmo, H.G., Cohen, P., Hormonal control of protein phosphorylation (1977) Adv Cycl Nuc Res, 8, pp. 145-251
- Krebs, E.G., Beavo, J.A., Phosphorylation-dephosphorylation of enzymes (1979) Ann Rev Biochem, 48, pp. 923-959
- Krebs, E.G., Historical perspectives on protein phosphorylation and a classification system for protein kinaes (1983) Phil-Trans-R. R Soc London, 302, pp. 3-1
- Judewicz, N.D., Glikin, G.C., Torres, H.N., Protein kinase activities in Neurospora crassa (1981) Arch Biochem Biophys, 206, pp. 87-92
- Glikin, G.C., Judewicz, N.D., Torres, H.N., Protein kinase activities in Neurospora crassa- Molecular Properties (1982) Mol Cell Biochem, 46, pp. 121-126
- Powers, P.A., Pall, M.L., Cyclic AMP-dependent protein kinase of Neurospora crassa (1980) Biochem Biophys Res Comm, 95, pp. 701-706
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
- Glynn, I.M., Chappell, J.B., A simple method for the preparation of (32P)-labelled adenosine triphosphate of high specific activity (1964) Biochem J, 90, pp. 147-149
- Téllez-Iñón, M.T., Ulloa, R.M., Glikin, G.C., Torres, H.N., Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin (1985) Biochem J, 232, pp. 425-430
Citas:
---------- APA ----------
Ulloa, R.M., Glikin, G.C., Téllez-Ĩnón, M.T., Torres, H.N. & Judewicz, N.D. (1987) . A novel stimulator of protein phosphorylation in Neurospora crassa. Molecular and Cellular Biochemistry, 77(1), 11-17.http://dx.doi.org/10.1007/BF00230146
---------- CHICAGO ----------
Ulloa, R.M., Glikin, G.C., Téllez-Ĩnón, M.T., Torres, H.N., Judewicz, N.D. "A novel stimulator of protein phosphorylation in Neurospora crassa" . Molecular and Cellular Biochemistry 77, no. 1 (1987) : 11-17.http://dx.doi.org/10.1007/BF00230146
---------- MLA ----------
Ulloa, R.M., Glikin, G.C., Téllez-Ĩnón, M.T., Torres, H.N., Judewicz, N.D. "A novel stimulator of protein phosphorylation in Neurospora crassa" . Molecular and Cellular Biochemistry, vol. 77, no. 1, 1987, pp. 11-17.http://dx.doi.org/10.1007/BF00230146
---------- VANCOUVER ----------
Ulloa, R.M., Glikin, G.C., Téllez-Ĩnón, M.T., Torres, H.N., Judewicz, N.D. A novel stimulator of protein phosphorylation in Neurospora crassa. Mol Cell Biochem. 1987;77(1):11-17.http://dx.doi.org/10.1007/BF00230146
